{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T01:41:27Z","timestamp":1774489287989,"version":"3.50.1"},"reference-count":74,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2012,8,15]],"date-time":"2012-08-15T00:00:00Z","timestamp":1344988800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Mangroves are complex ecosystems that regulate nutrient and sediment fluxes to the open sea. The importance of bacteria and fungi in regulating nutrient cycles has led to an interest in their diversity and composition in mangroves. However, very few studies have assessed\n            <jats:named-content content-type=\"genus-species\">Archaea<\/jats:named-content>\n            in mangroves, and virtually nothing is known about whether mangrove rhizospheres affect archaeal diversity and composition. Here, we studied the diversity and composition of\n            <jats:named-content content-type=\"genus-species\">Archaea<\/jats:named-content>\n            in mangrove bulk sediment and the rhizospheres of two mangrove trees,\n            <jats:named-content content-type=\"genus-species\">Rhizophora mangle<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Laguncularia racemosa<\/jats:named-content>\n            , using denaturing gradient gel electrophoresis (DGGE) and pyrosequencing of archaeal 16S rRNA genes with a nested-amplification approach. DGGE profiles revealed significant structural differences between bulk sediment and rhizosphere samples, suggesting that roots of both mangrove species influence the sediment archaeal community. Nearly all of the detected sequences obtained with pyrosequencing were identified as\n            <jats:named-content content-type=\"genus-species\">Archaea<\/jats:named-content>\n            , but most were unclassified at the level of phylum or below. Archaeal richness was, furthermore, the highest in the\n            <jats:named-content content-type=\"genus-species\">L. racemosa<\/jats:named-content>\n            rhizosphere, intermediate in bulk sediment, and the lowest in the\n            <jats:named-content content-type=\"genus-species\">R. mangle<\/jats:named-content>\n            rhizosphere. This study shows that rhizosphere microhabitats of\n            <jats:named-content content-type=\"genus-species\">R. mangle<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">L. racemosa<\/jats:named-content>\n            , common plants in subtropical mangroves located in Rio de Janeiro, Brazil, hosted distinct archaeal assemblages.\n          <\/jats:p>","DOI":"10.1128\/aem.00386-12","type":"journal-article","created":{"date-parts":[[2012,6,2]],"date-time":"2012-06-02T02:22:28Z","timestamp":1338603748000},"page":"5520-5528","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":224,"title":["Denaturing Gradient Gel Electrophoresis and Barcoded Pyrosequencing Reveal Unprecedented Archaeal Diversity in Mangrove Sediment and Rhizosphere Samples"],"prefix":"10.1128","volume":"78","author":[{"given":"Ana C. C.","family":"Pires","sequence":"first","affiliation":[{"name":"Department of Biology, CESAM, Universidade de Aveiro, Campus Universit\u00e1rio de Santiago, Aveiro, Portugal"}]},{"given":"Daniel F. R.","family":"Cleary","sequence":"additional","affiliation":[{"name":"Department of Biology, CESAM, Universidade de Aveiro, Campus Universit\u00e1rio de Santiago, Aveiro, Portugal"}]},{"given":"Adelaide","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Biology, CESAM, Universidade de Aveiro, Campus Universit\u00e1rio de Santiago, Aveiro, Portugal"}]},{"given":"\u00c2ngela","family":"Cunha","sequence":"additional","affiliation":[{"name":"Department of Biology, CESAM, Universidade de Aveiro, Campus Universit\u00e1rio de Santiago, Aveiro, Portugal"}]},{"given":"Simone","family":"Dealtry","sequence":"additional","affiliation":[{"name":"Julius K\u00fchn Institute for Cultivated Crops, Braunschweig, Germany"}]},{"given":"Leda C. S.","family":"Mendon\u00e7a-Hagler","sequence":"additional","affiliation":[{"name":"Institute of Microbiology Prof. Paulo de Go\u00e9s, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil"}]},{"given":"Kornelia","family":"Smalla","sequence":"additional","affiliation":[{"name":"Julius K\u00fchn Institute for Cultivated Crops, Braunschweig, Germany"}]},{"given":"Newton C. 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