{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T11:59:30Z","timestamp":1773835170142,"version":"3.50.1"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The origin, structure, and composition of biofilms in various compartments of an industrial full-scale reverse-osmosis (RO) membrane water purification plant were analyzed by molecular biological methods. Samples were taken when the RO installation suffered from a substantial pressure drop and decreased production. The bacterial community of the RO membrane biofilm was clearly different from the bacterial community present at other locations in the RO plant, indicating the development of a specialized bacterial community on the RO membranes. The typical freshwater phylotypes in the RO membrane biofilm (i.e.,\n            <jats:italic>Proteobacteria<\/jats:italic>\n            ,\n            <jats:italic>Cytophaga-Flexibacter-Bacteroides<\/jats:italic>\n            group, and\n            <jats:italic>Firmicutes<\/jats:italic>\n            ) were also present in the water sample fed to the plant, suggesting a feed water origin. However, the relative abundances of the different species in the mature biofilm were different from those in the feed water, indicating that the biofilm was actively formed on the RO membrane sheets and was not the result of a concentration of bacteria present in the feed water. The majority of the microorganisms (59% of the total number of clones) in the biofilm were related to the class\n            <jats:italic>Proteobacteria<\/jats:italic>\n            , with a dominance of\n            <jats:italic>Sphingomonas<\/jats:italic>\n            spp. (27% of all clones). Members of the genus\n            <jats:italic>Sphingomonas<\/jats:italic>\n            seem to be responsible for the biofouling of the membranes in the RO installation.\n          <\/jats:p>","DOI":"10.1128\/aem.00387-08","type":"journal-article","created":{"date-parts":[[2008,7,12]],"date-time":"2008-07-12T01:25:39Z","timestamp":1215825939000},"page":"5297-5304","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":124,"title":["Molecular Characterization of the Bacterial Communities in the Different Compartments of a Full-Scale Reverse-Osmosis Water Purification Plant"],"prefix":"10.1128","volume":"74","author":[{"given":"L. A.","family":"Bereschenko","sequence":"first","affiliation":[{"name":"Wetsus, P.O. 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