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The completely sequenced genome of\n            <jats:italic>Nostoc<\/jats:italic>\n            sp. strain PCC 7120 reveals that the five structural genes encoding the bidirectional hydrogenase (\n            <jats:italic>hoxEFUYH<\/jats:italic>\n            ) are separated in two clusters at a distance of approximately 8.8 kb. The transcription of the\n            <jats:italic>hox<\/jats:italic>\n            genes was examined under nitrogen-fixing conditions, and the results demonstrate that the cluster containing\n            <jats:italic>hoxE<\/jats:italic>\n            and\n            <jats:italic>hoxF<\/jats:italic>\n            can be transcribed as one polycistronic unit together with the open reading frame alr0750. The second cluster, containing\n            <jats:italic>hoxU<\/jats:italic>\n            ,\n            <jats:italic>hoxY<\/jats:italic>\n            , and\n            <jats:italic>hoxH<\/jats:italic>\n            , is transcribed together with alr0763 and alr0765, located between the\n            <jats:italic>hox<\/jats:italic>\n            genes. Moreover, alr0760 and alr0761 form an additional larger operon. Nevertheless, Northern blot hybridizations revealed a rather complex transcription pattern in which the different\n            <jats:italic>hox<\/jats:italic>\n            genes are expressed differently. Transcriptional start points (TSPs) were identified 66 and 57 bp upstream from the start codon of alr0750 and\n            <jats:italic>hoxU<\/jats:italic>\n            , respectively. The transcriptions of the two clusters containing the\n            <jats:italic>hox<\/jats:italic>\n            genes are both induced under anaerobic conditions concomitantly with the induction of a higher level of hydrogenase activity. An additional TSP, within the annotated alr0760, 244 bp downstream from the suggested translation start codon, was identified. Electrophoretic mobility shift assays with purified LexA from\n            <jats:italic>Nostoc<\/jats:italic>\n            sp. strain PCC 7120 demonstrated specific interactions between the transcriptional regulator and both\n            <jats:italic>hox<\/jats:italic>\n            promoter regions. However, when LexA from\n            <jats:italic>Synechocystis<\/jats:italic>\n            sp. strain PCC 6803 was used, the purified protein interacted only with the promoter region of the alr0750-\n            <jats:italic>hoxE<\/jats:italic>\n            -\n            <jats:italic>hoxF<\/jats:italic>\n            operon. A search of the whole\n            <jats:italic>Nostoc<\/jats:italic>\n            sp. strain PCC 7120 genome demonstrated the presence of 216 putative LexA binding sites in total, including\n            <jats:italic>recA<\/jats:italic>\n            and\n            <jats:italic>recF<\/jats:italic>\n            . This indicates that, in addition to the bidirectional hydrogenase gene, a number of other genes, including open reading frames connected to DNA replication, recombination, and repair, may be part of the LexA regulatory network in\n            <jats:italic>Nostoc<\/jats:italic>\n            sp. strain PCC 7120.\n          <\/jats:p>","DOI":"10.1128\/aem.00756-07","type":"journal-article","created":{"date-parts":[[2007,7,14]],"date-time":"2007-07-14T01:01:24Z","timestamp":1184374884000},"page":"5435-5446","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Transcription and Regulation of the Bidirectional Hydrogenase in the Cyanobacterium\n            <i>Nostoc<\/i>\n            sp. Strain PCC 7120"],"prefix":"10.1128","volume":"73","author":[{"given":"Johannes","family":"Sjo\u0308holm","sequence":"first","affiliation":[{"name":"Department of Photochemistry and Molecular Science, The A\u030angstro\u0308m Laboratories, Uppsala University, Box 523, SE-751 20 Uppsala, Sweden"}]},{"given":"Paulo","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Photochemistry and Molecular Science, The A\u030angstro\u0308m Laboratories, Uppsala University, Box 523, SE-751 20 Uppsala, Sweden"}]},{"given":"Peter","family":"Lindblad","sequence":"additional","affiliation":[{"name":"Department of Photochemistry and Molecular Science, The A\u030angstro\u0308m Laboratories, Uppsala University, Box 523, SE-751 20 Uppsala, Sweden"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijhydene.2006.06.037"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(96)00176-8"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030000139"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.22.7655-7666.2005"},{"key":"e_1_3_2_6_2","first-page":"49","volume":"888","year":"2003","unstructured":"Axelsson, R. 2003. 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