{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T21:46:36Z","timestamp":1774043196034,"version":"3.50.1"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"22","license":[{"start":{"date-parts":[[2007,11,15]],"date-time":"2007-11-15T00:00:00Z","timestamp":1195084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Fluorescence in situ hybridization (FISH) was used for direct detection of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            on pipe surfaces and coupons in drinking water distribution networks. Old cast iron main pipes were removed from water distribution networks in France, England, Portugal, and Latvia, and\n            <jats:italic>E. coli<\/jats:italic>\n            was analyzed in the biofilm. In addition, 44 flat coupons made of cast iron, polyvinyl chloride, or stainless steel were placed into and continuously exposed to water on 15 locations of 6 distribution networks in France and Latvia and examined after 1 to 6 months exposure to the drinking water. In order to increase the signal intensity, a peptide nucleic acid (PNA) 15-mer probe was used in the FISH screening for the presence or absence of\n            <jats:italic>E. coli<\/jats:italic>\n            on the surface of pipes and coupons, thus reducing occasional problems of autofluorescence and low fluorescence of the labeled bacteria. For comparison, cells were removed from the surfaces and examined with culture-based or enzymatic (detection of \u03b2-\n            <jats:sc>d<\/jats:sc>\n            -glucuronidase) methods. An additional verification was made by using PCR. Culture method indicated presence of\n            <jats:italic>E. coli<\/jats:italic>\n            in one of five pipes, whereas all pipes were positive with the FISH methods.\n            <jats:italic>E. coli<\/jats:italic>\n            was detected in 56% of the coupons using PNA FISH, but no\n            <jats:italic>E. coli<\/jats:italic>\n            was detected using culture or enzymatic methods. PCR analyses confirmed the presence of\n            <jats:italic>E. coli<\/jats:italic>\n            in samples that were negative according to culture-based and enzymatic methods. The viability of\n            <jats:italic>E. coli<\/jats:italic>\n            cells in the samples was demonstrated by the cell elongation after resuscitation in low-nutrient medium supplemented with pipemidic acid, suggesting that the cells were present in an active but nonculturable state, unable to grow on agar media.\n            <jats:italic>E. coli<\/jats:italic>\n            contributed to ca. 0.001 to 0.1% of the total bacterial number in the samples. The presence and number of\n            <jats:italic>E. coli<\/jats:italic>\n            did not correlate with any of physical and\/or chemical characteristic of the drinking water (e.g., temperature, chlorine, or biodegradable organic matter concentration). We show here that\n            <jats:italic>E. coli<\/jats:italic>\n            is present in the biofilms of drinking water networks in Europe. Some of the cells are metabolically active but are often not detected due to limitations of traditionally used culture-based methods, indicating that biofilm should be considered as a reservoir that must be investigated further in order to evaluate the risk for human health.\n          <\/jats:p>","DOI":"10.1128\/aem.00845-07","type":"journal-article","created":{"date-parts":[[2007,8,25]],"date-time":"2007-08-25T01:08:40Z","timestamp":1188004120000},"page":"7456-7464","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":93,"title":["Detection of\n            <i>Escherichia coli<\/i>\n            in Biofilms from Pipe Samples and Coupons in Drinking Water Distribution Networks"],"prefix":"10.1128","volume":"73","author":[{"given":"T.","family":"Juhna","sequence":"first","affiliation":[{"name":"Riga Technical University, Department of Water Engineering and Technology, Riga LV-1658, Latvia"}]},{"given":"D.","family":"Birzniece","sequence":"additional","affiliation":[{"name":"Riga Technical University, Department of Water Engineering and Technology, Riga LV-1658, Latvia"}]},{"given":"S.","family":"Larsson","sequence":"additional","affiliation":[{"name":"Riga Technical University, Department of Water Engineering and Technology, Riga LV-1658, Latvia"}]},{"given":"D.","family":"Zulenkovs","sequence":"additional","affiliation":[{"name":"Latvian Biomedical Research and Study Centre, Ratsupites 1, Riga LV-1067, Latvia"}]},{"given":"A.","family":"Sharipo","sequence":"additional","affiliation":[{"name":"Latvian Biomedical Research and Study Centre, Ratsupites 1, Riga LV-1067, Latvia"}]},{"given":"N. F.","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Centro de Engenharia Biolo\u0301gica, Universidade do Minho, 4710-057 Braga, Portugal"},{"name":"Environmental Healthcare Unit, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, United Kingdom"}]},{"given":"F.","family":"Me\u0301nard-Szczebara","sequence":"additional","affiliation":[{"name":"Veolia Environment, Research Center for Water, Chemin de la Digue, BP 76, 78603 Maisons-Laffitte, France"}]},{"given":"S.","family":"Castagnet","sequence":"additional","affiliation":[{"name":"Veolia Environment, Research Center for Water, Chemin de la Digue, BP 76, 78603 Maisons-Laffitte, France"}]},{"given":"C.","family":"Fe\u0301liers","sequence":"additional","affiliation":[{"name":"Veolia Environment, Research Center for Water, Chemin de la Digue, BP 76, 78603 Maisons-Laffitte, France"}]},{"given":"C. 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