{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:06:16Z","timestamp":1761894376932},"reference-count":25,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T00:00:00Z","timestamp":1157068800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The accumulation of compatible solutes was studied in the hyperthermophilic bacterium<jats:italic>Aquifex pyrophilus<\/jats:italic>as a function of the temperature and the NaCl concentration of the growth medium. Nuclear magnetic resonance analysis of cell extracts revealed the presence of \u03b1- and \u03b2-glutamate, di-mannosyl-di-<jats:italic>myo<\/jats:italic>-inositol phosphate, di-<jats:italic>myo<\/jats:italic>-inositol phosphate, and an additional compound here identified as 1-glyceryl-1-<jats:italic>myo<\/jats:italic>-inosityl phosphate. All solutes accumulated by<jats:italic>A. pyrophilus<\/jats:italic>are negatively charged at physiological pH. The intracellular levels of di-<jats:italic>myo<\/jats:italic>-inositol phosphate increased in response to supraoptimal growth temperature, while \u03b1- and \u03b2-glutamate accumulated in response to osmotic stress, especially at growth temperatures below the optimum. The newly discovered compound, 1-glyceryl-1-<jats:italic>myo<\/jats:italic>-inosityl phosphate, appears to play a double role in osmo- and thermoprotection, since its intracellular pool increased primarily in response to a combination of osmotic and heat stresses. This work also uncovered the nature of the unknown compound, previously detected in<jats:italic>Archaeoglobus fulgidus<\/jats:italic>(L. O. Martins et al., Appl. Environ. Microbiol. 63:896-902, 1997). The curious structural relationship between diglycerol phosphate (found only in<jats:italic>Archaeoglobus<\/jats:italic>species), di-<jats:italic>myo<\/jats:italic>-inositol phosphate (a canonical solute of hyperthermophiles), and the newly identified solute is highlighted. This is the first report on the occurrence of 1-glyceryl-1-<jats:italic>myo<\/jats:italic>-inosityl phosphate in living systems.<\/jats:p>","DOI":"10.1128\/aem.00852-06","type":"journal-article","created":{"date-parts":[[2006,9,6]],"date-time":"2006-09-06T23:18:42Z","timestamp":1157584722000},"page":"6169-6173","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Occurrence of 1-Glyceryl-1-<i>myo<\/i>-Inosityl Phosphate in Hyperthermophiles"],"prefix":"10.1128","volume":"72","author":[{"given":"Pedro","family":"Lamosa","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 Oeiras, Portugal"}]},{"given":"Lu\u00eds G.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 Oeiras, Portugal"}]},{"given":"Marta V.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 Oeiras, Portugal"}]},{"given":"L\u00edgia O.","family":"Martins","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, 2780-156 Oeiras, Portugal"}]},{"given":"Neil D. 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