{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:22:06Z","timestamp":1767961326755,"version":"3.49.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T00:00:00Z","timestamp":1225497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Caldicellulosiruptor saccharolyticus<\/jats:italic>\n            is an extremely thermophilic, gram-positive anaerobe which ferments cellulose-, hemicellulose- and pectin-containing biomass to acetate, CO\n            <jats:sub>2<\/jats:sub>\n            , and hydrogen. Its broad substrate range, high hydrogen-producing capacity, and ability to coutilize glucose and xylose make this bacterium an attractive candidate for microbial bioenergy production. Here, the complete genome sequence of\n            <jats:italic>C. saccharolyticus<\/jats:italic>\n            , consisting of a 2,970,275-bp circular chromosome encoding 2,679 predicted proteins, is described. Analysis of the genome revealed that\n            <jats:italic>C. saccharolyticus<\/jats:italic>\n            has an extensive polysaccharide-hydrolyzing capacity for cellulose, hemicellulose, pectin, and starch, coupled to a large number of ABC transporters for monomeric and oligomeric sugar uptake. The components of the Embden-Meyerhof and nonoxidative pentose phosphate pathways are all present; however, there is no evidence that an Entner-Doudoroff pathway is present. Catabolic pathways for a range of sugars, including rhamnose, fucose, arabinose, glucuronate, fructose, and galactose, were identified. These pathways lead to the production of NADH and reduced ferredoxin. NADH and reduced ferredoxin are subsequently used by two distinct hydrogenases to generate hydrogen. Whole-genome transcriptome analysis revealed that there is significant upregulation of the glycolytic pathway and an ABC-type sugar transporter during growth on glucose and xylose, indicating that\n            <jats:italic>C. saccharolyticus<\/jats:italic>\n            coferments these sugars unimpeded by glucose-based catabolite repression. The capacity to simultaneously process and utilize a range of carbohydrates associated with biomass feedstocks is a highly desirable feature of this lignocellulose-utilizing, biofuel-producing bacterium.\n          <\/jats:p>","DOI":"10.1128\/aem.00968-08","type":"journal-article","created":{"date-parts":[[2008,9,6]],"date-time":"2008-09-06T00:55:43Z","timestamp":1220662543000},"page":"6720-6729","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":136,"title":["Hydrogenomics of the Extremely Thermophilic Bacterium\n            <i>Caldicellulosiruptor saccharolyticus<\/i>"],"prefix":"10.1128","volume":"74","author":[{"given":"Harmen J. G.","family":"van de Werken","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands"}]},{"given":"Marcel R. A.","family":"Verhaart","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands"}]},{"given":"Amy L.","family":"VanFossen","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695-7905"}]},{"given":"Karin","family":"Willquist","sequence":"additional","affiliation":[{"name":"Applied Microbiology, Lund Institute of Technology, Lund University, SE-221 00 Lund, Sweden"}]},{"given":"Derrick L.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695-7905"}]},{"given":"Jason D.","family":"Nichols","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695-7905"}]},{"given":"Heleen P.","family":"Goorissen","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands"}]},{"given":"Emmanuel F.","family":"Mongodin","sequence":"additional","affiliation":[{"name":"Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201"}]},{"given":"Karen E.","family":"Nelson","sequence":"additional","affiliation":[{"name":"The J. Craig Venter Institute, 9712 Medical Center Drive, Rockville, Maryland 20850"}]},{"given":"Ed W. J.","family":"van Niel","sequence":"additional","affiliation":[{"name":"Applied Microbiology, Lund Institute of Technology, Lund University, SE-221 00 Lund, Sweden"}]},{"given":"Alfons J. M.","family":"Stams","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands"}]},{"given":"Donald E.","family":"Ward","sequence":"additional","affiliation":[{"name":"Genencor International, a Danisco Company, 925 Page Mill Road, Palo Alto, California 94304"}]},{"given":"Willem M.","family":"de Vos","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands"}]},{"given":"John","family":"van der Oost","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands"}]},{"given":"Robert M.","family":"Kelly","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695-7905"}]},{"given":"Serv\u00e9 W. 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