{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T00:47:05Z","timestamp":1771030025983,"version":"3.50.1"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2013,10,15]],"date-time":"2013-10-15T00:00:00Z","timestamp":1381795200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Despite the emergence of non-O157 Shiga toxin-producing\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            (STEC) infections,\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            serotype O157 is still the most commonly identified STEC in the world. It causes high morbidity and mortality and has been responsible for a number of outbreaks in many parts of the world. Various methods have been developed to detect this particular serotype, but standard bacteriological methods remain the gold standard. Here, we propose a new peptide nucleic acid fluorescence\n            <jats:italic>in situ<\/jats:italic>\n            hybridization (PNA-FISH) method for the rapid detection of\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            O157. Testing on 54 representative strains showed that the PNA probe is highly sensitive and specific to\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            O157. The method then was optimized for detection in food samples. Ground beef and unpasteurized milk samples were artificially contaminated with\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            O157 concentrations ranging from 1 \u00d7 10\n            <jats:sup>\u22122<\/jats:sup>\n            to 1 \u00d7 10\n            <jats:sup>2<\/jats:sup>\n            CFU per 25 g or ml of food. Samples were then preenriched and analyzed by both the traditional bacteriological method (ISO 16654:2001) and PNA-FISH. The PNA-FISH method performed well in both types of food matrices with a detection limit of 1 CFU\/25 g or ml of food samples. Tests on 60 food samples have shown a specificity value of 100% (95% confidence interval [CI], 82.83 to 100), a sensitivity of 97.22% (95% CI, 83.79 to 99.85%), and an accuracy of 98.33% (CI 95%, 83.41 to 99.91%). Results indicate that PNA-FISH performed as well as the traditional culture methods and can reduce the diagnosis time to 1 day.\n          <\/jats:p>","DOI":"10.1128\/aem.01009-13","type":"journal-article","created":{"date-parts":[[2013,8,10]],"date-time":"2013-08-10T01:33:13Z","timestamp":1376098393000},"page":"6293-6300","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Detection of Escherichia coli O157 by Peptide Nucleic Acid Fluorescence\n            <i>In Situ<\/i>\n            Hybridization (PNA-FISH) and Comparison to a Standard Culture Method"],"prefix":"10.1128","volume":"79","author":[{"given":"C.","family":"Almeida","sequence":"first","affiliation":[{"name":"IBB\u2013Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal"},{"name":"LEPAE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal"},{"name":"BioMode, Biomolecular Determination S.A., SpinPark\u2013Centro de Incuba\u00e7\u00e3o de Base Tecnol\u00f3gica, Avepark\u2013Zona Industrial da Gandra, Caldas das Taipas Guimar\u00e3es, Portugal"}]},{"given":"J. M.","family":"Sousa","sequence":"additional","affiliation":[{"name":"IBB\u2013Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal"}]},{"given":"R.","family":"Rocha","sequence":"additional","affiliation":[{"name":"BioMode, Biomolecular Determination S.A., SpinPark\u2013Centro de Incuba\u00e7\u00e3o de Base Tecnol\u00f3gica, Avepark\u2013Zona Industrial da Gandra, Caldas das Taipas Guimar\u00e3es, Portugal"}]},{"given":"L.","family":"Cerqueira","sequence":"additional","affiliation":[{"name":"BioMode, Biomolecular Determination S.A., SpinPark\u2013Centro de Incuba\u00e7\u00e3o de Base Tecnol\u00f3gica, Avepark\u2013Zona Industrial da Gandra, Caldas das Taipas Guimar\u00e3es, Portugal"}]},{"given":"S.","family":"Fanning","sequence":"additional","affiliation":[{"name":"UCD Centre for Food Safety, School of Public Health, Physiotherapy &amp; Population Science, UCD Centre for Molecular Innovation &amp; Drug Discovery, University College Dublin, Belfield, Dublin, Ireland"}]},{"given":"N. F.","family":"Azevedo","sequence":"additional","affiliation":[{"name":"LEPAE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal"},{"name":"BioMode, Biomolecular Determination S.A., SpinPark\u2013Centro de Incuba\u00e7\u00e3o de Base Tecnol\u00f3gica, Avepark\u2013Zona Industrial da Gandra, Caldas das Taipas Guimar\u00e3es, Portugal"}]},{"given":"M. J.","family":"Vieira","sequence":"additional","affiliation":[{"name":"IBB\u2013Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012588365-8\/50010-4"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2006-508"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60963-4"},{"key":"e_1_3_3_5_2","first-page":"1096","volume-title":"Current research, technology and education topics in applied microbiology and microbial biotechnology","author":"Robinson AL","year":"2010","unstructured":"RobinsonALMcKillipJ. 2010. Biology of Escherichia coli O157:H7 in human health and food safety with emphasis on sublethal injury and detection, p 1096\u20131105. 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