{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T17:35:24Z","timestamp":1770744924979,"version":"3.49.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:italic>Saccharomyces cerevisiae SPI1<\/jats:italic>\n            gene encodes a member of the glycosylphosphatidylinositol-anchored cell wall protein family. In this work we show results indicating that\n            <jats:italic>SPI1<\/jats:italic>\n            expression protects the yeast cell from damage caused by weak acids used as food preservatives. This is documented by a less extended period of adaptation to growth in their presence and by a less inhibited specific growth rate for a parental strain compared with a mutant with\n            <jats:italic>SPI1<\/jats:italic>\n            deleted. Maximal protection exerted by Spi1p against equivalent concentrations of the various weak acids tested was registered for the more lipophilic acids (octanoic acid, followed by benzoic acid) and was minimal for acetic acid. Weak-acid adaptation was found to involve the rapid activation of\n            <jats:italic>SPI1<\/jats:italic>\n            transcription, which is dependent on the presence of the Msn2p transcription factor. Activation of\n            <jats:italic>SPI1<\/jats:italic>\n            transcription upon acetic acid stress also requires Haa1p, whereas this recently described transcription factor has a negligible role in the adaptive response to benzoic acid. The expression of\n            <jats:italic>SPI1<\/jats:italic>\n            was found to play a prominent role in the development of yeast resistance to 1,3-\u03b2-glucanase in benzoic acid-stressed cells, while its involvement in acetic acid-induced resistance to the cell wall-lytic enzyme is slighter. The results are consistent with the notion that Spi1p expression upon weak-acid stress leads to cell wall remodeling, especially for the more lipophilic acids, decreasing cell wall porosity. Decreased cell wall porosity, in turn, reduces access to the plasma membrane, reducing membrane damage, intracellular acidification, and viability loss.\n          <\/jats:p>","DOI":"10.1128\/aem.01476-06","type":"journal-article","created":{"date-parts":[[2006,11,6]],"date-time":"2006-11-06T18:53:46Z","timestamp":1162839226000},"page":"7168-7175","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["The\n            <i>SPI1<\/i>\n            Gene, Encoding a Glycosylphosphatidylinositol-Anchored Cell Wall Protein, Plays a Prominent Role in the Development of Yeast Resistance to Lipophilic Weak-Acid Food Preservatives"],"prefix":"10.1128","volume":"72","author":[{"given":"T.","family":"Simo\u0303es","sequence":"first","affiliation":[{"name":"Biological Sciences Research Group, Centro de Engenharia Biolo\u0301gica e Qui\u0301mica, Instituto Superior Te\u0301cnico, 1049-001 Lisboa, Portugal"}]},{"given":"N. P.","family":"Mira","sequence":"additional","affiliation":[{"name":"Biological Sciences Research Group, Centro de Engenharia Biolo\u0301gica e Qui\u0301mica, Instituto Superior Te\u0301cnico, 1049-001 Lisboa, Portugal"}]},{"given":"A. R.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Biological Sciences Research Group, Centro de Engenharia Biolo\u0301gica e Qui\u0301mica, Instituto Superior Te\u0301cnico, 1049-001 Lisboa, Portugal"}]},{"given":"Isabel","family":"Sa\u0301-Correia","sequence":"additional","affiliation":[{"name":"Biological Sciences Research Group, Centro de Engenharia Biolo\u0301gica e Qui\u0301mica, Instituto Superior Te\u0301cnico, 1049-001 Lisboa, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M210715200"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307760200"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(96)00245-3"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.12.2.323"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-140-8-1881"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.9.2160"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2005.04.002"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.320060606"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.320070402"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.14.4.2740-2754.1994"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27551-0"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.09.010"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312954200"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.12.4241"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02242.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107131200"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2002.tb00613.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1349"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211604200"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.69.2.262-291.2005"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.15.3735-3740.1998"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-10-2635"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(20000130)16:2<139::AID-YEA512>3.0.CO;2-J"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.118.2.534-540.1974"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e03-05-0322"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.13.3381-3387.1998"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.7.4019-4028.2003"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-4-781"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2006.00041.x"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.2.779-783.1998"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.159.3.1018-1026.1984"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01476-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01476-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T21:04:00Z","timestamp":1645650240000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.01476-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11]]},"references-count":31,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1128\/AEM.01476-06"],"URL":"https:\/\/doi.org\/10.1128\/aem.01476-06","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11]]},"assertion":[{"value":"2006-06-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-09-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}