{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T23:07:19Z","timestamp":1768777639263,"version":"3.49.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2010,12,15]],"date-time":"2010-12-15T00:00:00Z","timestamp":1292371200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Throughout alcoholic fermentation, nitrogen depletion is one of the most important environmental stresses that can negatively affect the yeast metabolic activity and ultimately leads to fermentation arrest. Thus, the identification of the underlying effects and biomarkers of nitrogen limitation is valuable for controlling, and therefore optimizing, alcoholic fermentation. In this study, reactive oxygen species (ROS), plasma membrane integrity, and cell cycle were evaluated in a wine strain of\n            <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n            during alcoholic fermentation in nitrogen-limiting medium under anaerobic conditions. The results indicated that nitrogen limitation leads to an increase in ROS and that the superoxide anion is a minor component of the ROS, but there is increased activity of both Sod2p and Cta1p. Associated with these effects was a decrease in plasma membrane integrity and a persistent cell cycle arrest at G\n            <jats:sub>0<\/jats:sub>\n            \/G\n            <jats:sub>1<\/jats:sub>\n            phases. Moreover, under these conditions it appears that autophagy, evaluated by\n            <jats:italic>ATG8<\/jats:italic>\n            expression, is induced, suggesting that this mechanism is essential for cell survival but does not prevent the cell cycle arrest observed in slow fermentation. Conversely, nitrogen refeeding allowed cells to reenter cell cycle by decreasing ROS generation and autophagy. Altogether, the results provide new insights on the understanding of wine fermentations under nitrogen-limiting conditions and further indicate that ROS accumulation, evaluated by the MitoTracker Red dye CM-H\n            <jats:sub>2<\/jats:sub>\n            XRos, and plasma membrane integrity could be useful as predictive markers of fermentation problems.\n          <\/jats:p>","DOI":"10.1128\/aem.01535-10","type":"journal-article","created":{"date-parts":[[2010,10,16]],"date-time":"2010-10-16T03:11:41Z","timestamp":1287198701000},"page":"7918-7924","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Accumulation of Non-Superoxide Anion Reactive Oxygen Species Mediates Nitrogen-Limited Alcoholic Fermentation by\n            <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Ana","family":"Mendes-Ferreira","sequence":"first","affiliation":[{"name":"Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, Universidade de Tra\u0301s-os-Montes e Alto Douro, Vila Real, Portugal"}]},{"given":"Bele\u0301m","family":"Sampaio-Marques","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Catarina","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, Universidade de Tra\u0301s-os-Montes e Alto Douro, Vila Real, Portugal"}]},{"given":"Fernando","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Vi\u0301tor","family":"Costa","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, 4150-180 Porto, Portugal"},{"name":"Instituto de Cie\u0302ncias Biome\u0301dicas Abel Salazar (ICBAS), Departamento de Biologia Molecular, Universidade do Porto, 4099-003 Porto, Portugal"}]},{"given":"Arlete","family":"Mendes-Faia","sequence":"additional","affiliation":[{"name":"Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, Universidade de Tra\u0301s-os-Montes e Alto Douro, Vila Real, Portugal"}]},{"given":"Paula","family":"Ludovico","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]},{"given":"Ceci\u0301lia","family":"Lea\u0303o","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05016-3"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700816"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2006.00188.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1297-1351"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2001.tb00022.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb07985.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2007.03.004"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.320080104"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05013-8"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.23.7196"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.12.4241"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2008.00371.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.35.22480"},{"key":"e_1_3_2_15_2","first-page":"77","year":"1993","unstructured":"Henschke, P. A., and V. Jiranek. 1993. Yeast: metabolism of nitrogen compounds, p. 77-165. In G. H. Fleet (ed.), Wine microbiology and biotechnology. Harwood Academic, Chur, Switzerland.","journal-title":"Wine microbiology and biotechnology"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(199812)14:16<1511::AID-YEA356>3.0.CO;2-S"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.035428"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2008.03.008"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e01-12-0161"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-12-3335"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-1356(02)00201-5"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-009-0527-x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02754-06"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2004.02331.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1004432107"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac60147a030"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.49.1.249"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1999.0501"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.5.2123-2128.2001"},{"key":"e_1_3_2_30_2","unstructured":"Sugar metabolism in yeast: an overview of aerobic and anaerobic glucose catabolism. 2006"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1046"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0922-338X(98)80054-8"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.2.301"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj20020140"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(98)80067-7"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1217"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.57.2.383-401.1993"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.1.836-847.2006"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01535-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01535-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T17:17:59Z","timestamp":1645636679000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.01535-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12,15]]},"references-count":38,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2010,12,15]]}},"alternative-id":["10.1128\/AEM.01535-10"],"URL":"https:\/\/doi.org\/10.1128\/aem.01535-10","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12,15]]},"assertion":[{"value":"2010-06-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-10-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-12-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}