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The methodology established took into account the screening for biochemical traits desirable in a yeast cachac\u0327a producer, such as no H<jats:sub>2<\/jats:sub>S production, high tolerance to ethanol and high temperatures, high fermentative capacity, and the abilities to flocculate and to produce mycocins. Furthermore, the yeasts were exposed to drugs such as 5,5\u2032,5\"-trifluor-<jats:sc>d<\/jats:sc>,<jats:sc>l<\/jats:sc>-leucine and cerulenin to isolate those that potentially overproduce higher alcohols and esters. The utilization of a random amplified polymorphic DNA-PCR method with primers based on intron splicing sites flanking regions of the<jats:italic>COX1<\/jats:italic>gene, as well as microsatellite analysis, was not sufficient to achieve good differentiation among selected strains. In contrast, karyotype analysis allowed a clear distinction among all strains. Two selected strains were experimentally evaluated as cachac\u0327a producers. The results suggest that the selection of strains as fermentation starters requires the combined use of biochemical and molecular criteria to ensure the isolation and identification of strains with potential characteristics to produce cachac\u0327a with a higher quality standard.<\/jats:p>","DOI":"10.1128\/aem.01729-07","type":"journal-article","created":{"date-parts":[[2007,12,8]],"date-time":"2007-12-08T01:53:55Z","timestamp":1197078835000},"page":"693-701","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Biochemical and Molecular Characterization of<i>Saccharomyces cerevisiae<\/i>Strains Obtained from Sugar-Cane Juice Fermentations and Their Impact in Cachac\u0327a Production"],"prefix":"10.1128","volume":"74","author":[{"given":"Valdine\u0301ia Aparecida","family":"Oliveira","sequence":"first","affiliation":[{"name":"Laborato\u0301rio de Biologia Celular e Molecular, Nu\u0301cleo de Pesquisas em Cie\u0302ncias Biolo\u0301gicas, Departamento de Farma\u0301cia, Escola de Farma\u0301cia, Universidade Federal de Ouro Preto"}]},{"given":"Maristela Arau\u0301jo","family":"Vicente","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Biologia Celular e Molecular, Nu\u0301cleo de Pesquisas em Cie\u0302ncias Biolo\u0301gicas, Departamento de Farma\u0301cia, Escola de Farma\u0301cia, Universidade Federal de Ouro Preto"}]},{"given":"Luciano Gomes","family":"Fietto","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Biologia Celular e Molecular, Nu\u0301cleo de Pesquisas em Cie\u0302ncias Biolo\u0301gicas, Departamento de Farma\u0301cia, Escola de Farma\u0301cia, Universidade Federal de Ouro Preto"}]},{"given":"Ieso","family":"de Miranda Castro","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Biologia Celular e Molecular, Nu\u0301cleo de Pesquisas em Cie\u0302ncias Biolo\u0301gicas, Departamento de Farma\u0301cia, Escola de Farma\u0301cia, Universidade Federal de Ouro Preto"}]},{"given":"Mauri\u0301cio Xavier","family":"Coutrim","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Ana\u0301lises Fi\u0301sico-Qui\u0301micas, Departamento de Qui\u0301mica, Instituto de Cie\u0302ncias Exatas e Biolo\u0301gicas, Universidade Federal de Ouro Preto, 35.400-000 Ouro Preto, Minas Gerais, Brazil"}]},{"given":"Dorit","family":"Schu\u0308ller","sequence":"additional","affiliation":[{"name":"Centro de Biologia, Departamento de Biologia, Universidade do Minho, 4710-057 Braga, Portugal"}]},{"given":"Henrique","family":"Alves","sequence":"additional","affiliation":[{"name":"Centro de Biologia, Departamento de Biologia, Universidade do Minho, 4710-057 Braga, Portugal"}]},{"given":"Margarida","family":"Casal","sequence":"additional","affiliation":[{"name":"Centro de Biologia, Departamento de Biologia, Universidade do Minho, 4710-057 Braga, Portugal"}]},{"given":"Juliana","family":"de Oliveira Santos","sequence":"additional","affiliation":[{"name":"Centro de Cie\u0302ncias Exatas e Tecnolo\u0301gicas, Departamento de Tecnologia de Alimentos, Universidade Federal de Vic\u0327osa, 36.570-000 Vic\u0327osa, Minas Gerais, Brazil"}]},{"given":"Leandro Dias","family":"Arau\u0301jo","sequence":"additional","affiliation":[{"name":"Centro de Cie\u0302ncias Exatas e Tecnolo\u0301gicas, Departamento de Tecnologia de Alimentos, Universidade Federal de Vic\u0327osa, 36.570-000 Vic\u0327osa, Minas Gerais, Brazil"}]},{"given":"Paulo Henrique Alves","family":"da Silva","sequence":"additional","affiliation":[{"name":"Centro de Cie\u0302ncias Exatas e Tecnolo\u0301gicas, Departamento de Tecnologia de Alimentos, Universidade Federal de Vic\u0327osa, 36.570-000 Vic\u0327osa, Minas Gerais, Brazil"}]},{"given":"Rogelio Lopes","family":"Branda\u0303o","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Biologia Celular e Molecular, Nu\u0301cleo de Pesquisas em Cie\u0302ncias Biolo\u0301gicas, Departamento de Farma\u0301cia, Escola de Farma\u0301cia, Universidade Federal de Ouro Preto"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"2061","volume":"51","year":"1987","unstructured":"Ashida, S., E. 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