{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T22:14:44Z","timestamp":1775168084091,"version":"3.50.1"},"reference-count":80,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>\n            serovar Typhimurium is a Gram-negative bacterium able to invade and replicate inside eukaryotic cells. To cope with the host defense mechanisms, the bacterium has to rapidly remodel its transcriptional status. Regulatory RNAs and ribonucleases are the factors that ultimately control the fate of mRNAs and final protein levels in the cell. There is growing evidence of the direct involvement of these factors in bacterial pathogenicity. In this report, we validate the use of a\n            <jats:named-content content-type=\"genus-species\">Galleria mellonela<\/jats:named-content>\n            model in\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium pathogenicity studies through the parallel analysis of a mutant with a mutation in\n            <jats:italic>hfq<\/jats:italic>\n            , a well-established\n            <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n            virulence gene. The results obtained with this mutant are similar to the ones reported in a mouse model. Through the use of this insect model, we demonstrate a role for the main endoribonucleases RNase E and RNase III in\n            <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n            virulence. These ribonuclease mutants show an attenuated virulence phenotype, impairment in motility, and reduced proliferation inside the host. Interestingly, the two mutants trigger a distinct immune response in the host, and the two mutations seem to have an impact on distinct bacterial functions.\n          <\/jats:p>","DOI":"10.1128\/aem.02044-13","type":"journal-article","created":{"date-parts":[[2013,8,3]],"date-time":"2013-08-03T05:06:27Z","timestamp":1375506387000},"page":"6124-6133","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["The Virulence of Salmonella enterica Serovar Typhimurium in the Insect Model Galleria mellonella Is Impaired by Mutations in RNase E and RNase III"],"prefix":"10.1128","volume":"79","author":[{"given":"Sandra C.","family":"Viegas","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Oeiras, Portugal"}]},{"given":"Dalila","family":"Mil-Homens","sequence":"additional","affiliation":[{"name":"IBB\u2014Institute for Biotechnology and Bioengineering, Center for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Lisbon, Portugal"},{"name":"Department of Bioengineering, Instituto Superior T\u00e9cnico, Lisbon, Portugal"}]},{"given":"Ars\u00e9nio M.","family":"Fialho","sequence":"additional","affiliation":[{"name":"IBB\u2014Institute for Biotechnology and Bioengineering, Center for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Lisbon, Portugal"},{"name":"Department of Bioengineering, Instituto Superior T\u00e9cnico, Lisbon, Portugal"}]},{"given":"Cec\u00edlia M.","family":"Arraiano","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Oeiras, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01411-3"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2011.02.007"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03313.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.01099.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.08.022"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2010.00242.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2013.03.009"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1127103"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.94"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki313"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600572"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.603108"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2004.12.003"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq1242"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002520"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1113521108"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002782"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.4161\/rna.6915"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm916"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq1239"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.423507"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07161.x"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.026823-0"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2003.09.002"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2008.08.014"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2004.00116.x"},{"key":"e_1_3_3_28_2","first-page":"776","article-title":"Insect infection model for Campylobacter jejuni reveals that O-methyl phosphoramidate has insecticidal activity","volume":"201","author":"Champion OL","year":"2010","unstructured":"ChampionOLKarlyshevAVSeniorNJWoodwardMLa RagioneRHowardSLWrenBWTitballRW. 2010. Insect infection model for Campylobacter jejuni reveals that O-methyl phosphoramidate has insecticidal activity. J. Infect. Dis. 201:776\u2013782.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.13.3843-3845.2000"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.040782-0"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.2.2.14338"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.1.6.12985"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2008.02.011"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00327.x"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2011.02476.x"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2010.05.002"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01465.x"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1330405"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02186.x"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.5.2581-2590.1989"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb12858.x"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.8.4099-4105.1999"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2005.01.014"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000163"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05489.x"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2009.09.004"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2006.03.002"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2009.01.014"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268899002460"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00173"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4281787.x"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2008.01089.x"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.18.6580-6583.2005"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/291238a0"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1139\/m68-039"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04142.x"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006213"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01629.x"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0882-4010(03)00149-9"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-7915.2010.00212.x"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01301-09"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00510-12"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01991.x"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.d01-1718.x"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.mmi_17010169.x"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00640-07"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27520-0"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04293.x"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.9.5619-5625.2001"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1346"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1344"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00898-07"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.04901-0"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01341-08"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046828"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.5.3021-3030.2001"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2003.08.005"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.133.3.1543-1545.1978"},{"key":"e_1_3_3_80_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.10.4625-4633.1988"},{"key":"e_1_3_3_81_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90366-J"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02044-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.02044-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T19:54:45Z","timestamp":1645646085000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.02044-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10]]},"references-count":80,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["10.1128\/AEM.02044-13"],"URL":"https:\/\/doi.org\/10.1128\/aem.02044-13","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10]]},"assertion":[{"value":"2013-06-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-07-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}