{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T12:47:44Z","timestamp":1768913264501,"version":"3.49.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2008,3,15]],"date-time":"2008-03-15T00:00:00Z","timestamp":1205539200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2008,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Candida arabinofermentans<\/jats:italic>\n            PYCC 5603\n            <jats:sup>T<\/jats:sup>\n            and\n            <jats:italic>Pichia guilliermondii<\/jats:italic>\n            PYCC 3012 were shown to grow well on\n            <jats:sc>l<\/jats:sc>\n            -arabinose, albeit exhibiting distinct features that justify an in-depth comparative study of their respective pentose catabolism. Carbon-13 labeling experiments coupled with in vivo nuclear magnetic resonance (NMR) spectroscopy were used to investigate\n            <jats:sc>l<\/jats:sc>\n            -arabinose metabolism in these yeasts, thereby complementing recently reported physiological and enzymatic data. The label supplied in\n            <jats:sc>l<\/jats:sc>\n            -[2-\n            <jats:sup>13<\/jats:sup>\n            C]arabinose to nongrowing cells, under aerobic conditions, was found on C-1 and C-2 of arabitol and ribitol, on C-2 of xylitol, and on C-1, C-2, and C-3 of trehalose. The detection of labeled arabitol and xylitol constitutes additional evidence for the operation in yeast of the redox catabolic pathway, which is widespread among filamentous fungi. Furthermore, labeling at position C-1 of trehalose and arabitol demonstrates that glucose-6-phosphate is recycled through the oxidative pentose phosphate pathway (PPP). This result was interpreted as a metabolic strategy to regenerate NADPH, the cofactor essential for sustaining\n            <jats:sc>l<\/jats:sc>\n            -arabinose catabolism at the level of\n            <jats:sc>l<\/jats:sc>\n            -arabinose reductase and\n            <jats:sc>l<\/jats:sc>\n            -xylulose reductase. Moreover, the observed synthesis of\n            <jats:sc>d<\/jats:sc>\n            -arabitol and ribitol provides a route with which to supply NAD\n            <jats:sup>+<\/jats:sup>\n            under oxygen-limiting conditions. In\n            <jats:italic>P. guilliermondii<\/jats:italic>\n            PYCC 3012, the strong accumulation of\n            <jats:sc>l<\/jats:sc>\n            -arabitol (intracellular concentration of up to 0.4 M) during aerobic\n            <jats:sc>l<\/jats:sc>\n            -arabinose metabolism indicates the existence of a bottleneck at the level of\n            <jats:sc>l<\/jats:sc>\n            -arabitol 4-dehydrogenase. This report provides the first experimental evidence for a link between\n            <jats:sc>l<\/jats:sc>\n            -arabinose metabolism in fungi and the oxidative branch of the PPP and suggests rational guidelines for the design of strategies for the production of new and efficient\n            <jats:sc>l<\/jats:sc>\n            -arabinose-fermenting yeasts.\n          <\/jats:p>","DOI":"10.1128\/aem.02453-07","type":"journal-article","created":{"date-parts":[[2008,2,2]],"date-time":"2008-02-02T02:07:33Z","timestamp":1201918053000},"page":"1845-1855","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Use of In Vivo\n            <sup>13<\/sup>\n            C Nuclear Magnetic Resonance Spectroscopy To Elucidate\n            <scp>l<\/scp>\n            -Arabinose Metabolism in Yeasts"],"prefix":"10.1128","volume":"74","author":[{"given":"Ce\u0301sar","family":"Fonseca","sequence":"first","affiliation":[{"name":"Centro de Recursos Microbiolo\u0301gicos, Department of Life Sciences, Faculdade de Cie\u0302ncias e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Ana Rute","family":"Neves","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, 2780-156 Oeiras, Portugal"}]},{"given":"Alexandra M. M.","family":"Antunes","sequence":"additional","affiliation":[{"name":"REQUIMTE, CQFB, Faculdade de Cie\u0302ncias e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Joa\u0303o Paulo","family":"Noronha","sequence":"additional","affiliation":[{"name":"REQUIMTE, CQFB, Faculdade de Cie\u0302ncias e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Ba\u0308rbel","family":"Hahn-Ha\u0308gerdal","sequence":"additional","affiliation":[{"name":"Department of Applied Microbiology, Lund University, SE-221 00 Lund, Sweden"}]},{"given":"Helena","family":"Santos","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, 2780-156 Oeiras, Portugal"}]},{"given":"Isabel","family":"Spencer-Martins","sequence":"additional","affiliation":[{"name":"Centro de Recursos Microbiolo\u0301gicos, Department of Life Sciences, Faculdade de Cie\u0302ncias e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2318(08)60337-6"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.7.4144-4150.2003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00693408"},{"key":"e_1_3_2_5_2","first-page":"965","volume":"129","year":"1983","unstructured":"Bruinenberg, P. 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