{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T15:32:32Z","timestamp":1770478352989,"version":"3.49.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2009,5,1]],"date-time":"2009-05-01T00:00:00Z","timestamp":1241136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of\n            <jats:italic>Cronobacter<\/jats:italic>\n            strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of\n            <jats:italic>Cronobacter<\/jats:italic>\n            in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.\n          <\/jats:p>","DOI":"10.1128\/aem.02470-08","type":"journal-article","created":{"date-parts":[[2009,3,7]],"date-time":"2009-03-07T02:10:18Z","timestamp":1236391818000},"page":"2925-2930","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of\n            <i>Cronobacter<\/i>\n            Genomospecies (\n            <i>Enterobacter sakazakii<\/i>\n            ) in Powdered Infant Formula"],"prefix":"10.1128","volume":"75","author":[{"given":"C.","family":"Almeida","sequence":"first","affiliation":[{"name":"IBB\u2014Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"Environmental Healthcare Unit, School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX Southampton, United Kingdom"}]},{"given":"N. F.","family":"Azevedo","sequence":"additional","affiliation":[{"name":"IBB\u2014Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal"},{"name":"Environmental Healthcare Unit, School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX Southampton, United Kingdom"}]},{"given":"C.","family":"Iversen","sequence":"additional","affiliation":[{"name":"Centres for Food Safety &amp; Food-borne Zoonomics, UCD Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland"}]},{"given":"S.","family":"Fanning","sequence":"additional","affiliation":[{"name":"Centres for Food Safety &amp; Food-borne Zoonomics, UCD Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland"}]},{"given":"C. W.","family":"Keevil","sequence":"additional","affiliation":[{"name":"Environmental Healthcare Unit, School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX Southampton, United Kingdom"}]},{"given":"M. J.","family":"Vieira","sequence":"additional","affiliation":[{"name":"IBB\u2014Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1888"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1997.tb00308.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkf450"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1605(94)90218-6"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.9.5451-5457.2005"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000253219.06258.23"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/524737"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki038"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-100"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.1.130-138.2003"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2006.02.008"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-30-3-569"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740-8709.2004.00008.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1136\/fn.88.5.F434"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00858-07"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2005.02.013"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4855-4857.2005"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01562-06"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2004.01507.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-7-64"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.65577-0"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.2721-2729.2006"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:BILE.0000003978.72266.96"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-200103000-00004"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-15"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-4-43"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.02.009"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.06.007"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2005.08.004"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-68.8.1623"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.2539-2546.2006"},{"key":"e_1_3_2_33_2","first-page":"137","volume":"59","year":"2007","unstructured":"Mullane, N. 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