{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T05:56:06Z","timestamp":1763704566150},"reference-count":49,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2009,7,15]],"date-time":"2009-07-15T00:00:00Z","timestamp":1247616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2009,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An open mixed culture was enriched with glycogen-accumulating organisms (GAOs) by using a sequencing batch reactor and treating an agroindustrial waste (sugar cane molasses) under cyclic anaerobic-aerobic conditions. Over a 1-year operating period, the culture exhibited a very stable GAO phenotype with an average polyhydroxyalkanoate (PHA) content of 17% total suspended solids. However, the GAO microbial community evolved over the course of operation to a culture exhibiting unusual characteristics in producing PHAs comprised of short-chain-length monomers, namely, 3-hydroxybutyrate, 3-hydroxy-2-methylbutyrate, 3-hydroxyvalerate, and 3-hydroxy-2-methylvalerate, and also, up to 31 mol% of the medium-chain-length (MCL) monomer 3-hydroxyhexanoate (3HHx). Microbial community analysis by fluorescence in situ hybridization revealed a concurrent long-term drift in the GAO community balance, from mainly \u201c\n            <jats:italic>Candidatus<\/jats:italic>\n            Competibacter phosphatis\u201d to mainly\n            <jats:italic>Defluviicoccus vanus<\/jats:italic>\n            -related organisms. The production of 3HHx was confirmed by\n            <jats:sup>13<\/jats:sup>\n            C nuclear magnetic resonance (NMR) and appeared to be related to the increased presence of\n            <jats:italic>D. vanus<\/jats:italic>\n            -related GAOs. These results suggest a broadened spectrum of material, chemical, and mechanical properties that can be achieved for biopolymers produced by open mixed cultures from fermented waste. The increased spectrum of polymer properties brings a wider scope of potential applications.\n          <\/jats:p>","DOI":"10.1128\/aem.02486-08","type":"journal-article","created":{"date-parts":[[2009,5,23]],"date-time":"2009-05-23T01:27:24Z","timestamp":1243042044000},"page":"4676-4686","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Community Structure Evolution and Enrichment of Glycogen-Accumulating Organisms Producing Polyhydroxyalkanoates from Fermented Molasses"],"prefix":"10.1128","volume":"75","author":[{"given":"Ana R.","family":"Pisco","sequence":"first","affiliation":[{"name":"REQUIMTE\/CQFB, Chemistry Department, FCT\/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Simon","family":"Bengtsson","sequence":"additional","affiliation":[{"name":"AnoxKaldnes AB, Klostera\u0308ngsva\u0308gen 11A, SE-226 47 Lund, Sweden"}]},{"given":"Alan","family":"Werker","sequence":"additional","affiliation":[{"name":"AnoxKaldnes AB, Klostera\u0308ngsva\u0308gen 11A, SE-226 47 Lund, Sweden"}]},{"given":"Maria A. 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