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In this study, the microbial compositions of the different granules were analyzed by molecular microbiological techniques: cloning, denaturing gradient gel electrophoresis and fluorescent in situ hybridization (FISH), and scanning and transmission electron microscopy. We propose here that the different types of granules reflect the different stages in the life cycle of granules. Young granules were small, black, and compact and harbored active cells. Gray granules were the most abundant granules. These granules have a multilayer structure with channels and void areas. The core was composed of dead or starving cells with low activity. The brown granules, which were the largest granules, showed a loose and amorphous structure with big channels that resulted in fractured zones and corresponded to the older granules.<jats:italic>Firmicutes<\/jats:italic>(as determined by FISH) and<jats:italic>Nitrospira<\/jats:italic>and<jats:italic>Deferribacteres<\/jats:italic>(as determined by cloning and sequencing) were the predominant<jats:italic>Bacteria<\/jats:italic>. Remarkably,<jats:italic>Firmicutes<\/jats:italic>could not be detected in the brown granules. The methanogenic<jats:italic>Archaea<\/jats:italic>identified were<jats:italic>Methanosaeta concilii<\/jats:italic>(70 to 90% by FISH and cloning),<jats:italic>Methanosarcina mazei<\/jats:italic>, and<jats:italic>Methanospirillum<\/jats:italic>spp. The phenotypic appearance of the granules reflected the physiological condition of the granules. This may be valuable to easily select appropriate seed sludges to start up other reactors.<\/jats:p>","DOI":"10.1128\/aem.02985-05","type":"journal-article","created":{"date-parts":[[2006,7,7]],"date-time":"2006-07-07T01:51:13Z","timestamp":1152237073000},"page":"4942-4949","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":133,"title":["Phenotypic Properties and Microbial Diversity of Methanogenic Granules from a Full-Scale Upflow Anaerobic Sludge Bed Reactor Treating Brewery Wastewater"],"prefix":"10.1128","volume":"72","author":[{"given":"Emiliano E.","family":"Di\u0301az","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Autonoma University of Madrid, 28049 Madrid, Spain"}]},{"given":"Alfons J. 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