{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:05:33Z","timestamp":1759104333618},"reference-count":50,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2011,8,15]],"date-time":"2011-08-15T00:00:00Z","timestamp":1313366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2011,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Phytosanitary regulations and the provision of plant health certificates still rely mainly on long and laborious culture-based methods of diagnosis, which are frequently inconclusive. DNA-based methods of detection can circumvent many of the limitations of currently used screening methods, allowing a fast and accurate monitoring of samples. The genus\n            <jats:named-content content-type=\"genus-species\">Xanthomonas<\/jats:named-content>\n            includes 13 phytopathogenic quarantine organisms for which improved methods of diagnosis are needed. In this work, we propose 21 new\n            <jats:named-content content-type=\"genus-species\">Xanthomonas<\/jats:named-content>\n            -specific molecular markers, within loci coding for\n            <jats:named-content content-type=\"genus-species\">Xanthomonas<\/jats:named-content>\n            -specific protein domains, useful for DNA-based methods of identification of xanthomonads. The specificity of these markers was assessed by a dot blot hybridization array using 23 non-\n            <jats:named-content content-type=\"genus-species\">Xanthomonas<\/jats:named-content>\n            species, mostly soil dwelling and\/or phytopathogens for the same host plants. In addition, the validation of these markers on 15\n            <jats:named-content content-type=\"genus-species\">Xanthomonas<\/jats:named-content>\n            spp. suggested species-specific hybridization patterns, which allowed discrimination among the different\n            <jats:named-content content-type=\"genus-species\">Xanthomonas<\/jats:named-content>\n            species. Having in mind that DNA-based methods of diagnosis are particularly hampered for unsequenced species, namely,\n            <jats:named-content content-type=\"genus-species\">Xanthomonas fragariae<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Xanthomonas axonopodis<\/jats:named-content>\n            pv. phaseoli, and\n            <jats:named-content content-type=\"genus-species\">Xanthomonas fuscans<\/jats:named-content>\n            subsp.\n            <jats:named-content content-type=\"genus-species\">fuscans<\/jats:named-content>\n            , for which comparative genomics tools to search for DNA signatures are not yet applicable, emphasis was given to the selection of informative markers able to identify\n            <jats:named-content content-type=\"genus-species\">X. fragariae<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">X. axonopodis<\/jats:named-content>\n            pv. phaseoli, and\n            <jats:named-content content-type=\"genus-species\">X. fuscans<\/jats:named-content>\n            subsp.\n            <jats:named-content content-type=\"genus-species\">fuscans<\/jats:named-content>\n            strains. In order to avoid inconsistencies due to operator-dependent interpretation of dot blot data, an image-processing algorithm was developed to analyze automatically the dot blot patterns. Ultimately, the proposed markers and the dot blot platform, coupled with automatic data analyses, have the potential to foster a thorough monitoring of phytopathogenic xanthomonads.\n          <\/jats:p>","DOI":"10.1128\/aem.05189-11","type":"journal-article","created":{"date-parts":[[2011,6,25]],"date-time":"2011-06-25T23:16:27Z","timestamp":1309043787000},"page":"5619-5628","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Identification of Xanthomonas fragariae, Xanthomonas axonopodis pv. phaseoli, and Xanthomonas fuscans subsp. fuscans with Novel Markers and Using a Dot Blot Platform Coupled with Automatic Data Analysis"],"prefix":"10.1128","volume":"77","author":[{"given":"Pedro","family":"Albuquerque","sequence":"first","affiliation":[{"name":"Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal"},{"name":"Faculdade de Ci\u00eancias, Departamento de Biologia (FCUP), Edif\u00edcio FC4, Via Panor\u00e2mica no. 36, Universidade do Porto, 4150-564 Porto, Portugal"}]},{"given":"Cristina M. R.","family":"Caridade","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o em Ci\u00eancias Geo-Espaciais (CICGE), Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal"},{"name":"Instituto Superior de Engenharia de Coimbra (ISEC), Rua Pedro Nunes-Quinta da Nora, 3030-199 Coimbra, Portugal"}]},{"given":"Andre R. 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