{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T17:05:12Z","timestamp":1776186312228,"version":"3.50.1"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            pBL1 is a\n            <jats:named-content content-type=\"genus-species\">Lactococcus lactis<\/jats:named-content>\n            theta-replicating 10.9-kbp plasmid that encodes the synthetic machinery of the bacteriocin Lcn972. In this work, the transcriptomes of exponentially growing\n            <jats:named-content content-type=\"genus-species\">L. lactis<\/jats:named-content>\n            strains with and without pBL1 were compared. A discrete response was observed, with a total of 10 genes showing significantly changed expression. Upregulation of the lactococcal oligopeptide uptake (\n            <jats:italic>opp<\/jats:italic>\n            ) system was observed, which was likely linked to a higher nitrogen demand required for Lcn972 biosynthesis. Strikingly,\n            <jats:italic>celB<\/jats:italic>\n            , coding for the membrane porter IIC of the cellobiose phosphoenolpyruvate-dependent phosphotransferase system (PTS), and the upstream gene\n            <jats:italic>llmg0186<\/jats:italic>\n            were downregulated. Growth profiles for\n            <jats:named-content content-type=\"genus-species\">L. lactis<\/jats:named-content>\n            strains MG1363, MG1363\/pBL1, and MG1363 \u0394\n            <jats:italic>celB<\/jats:italic>\n            grown in chemically defined medium (CDM) containing cellobiose confirmed slower growth of MG1363\/pBL1 and MG1363 \u0394\n            <jats:italic>celB<\/jats:italic>\n            , while no differences were observed with growth on glucose. The presence of pBL1 shifted the fermentation products toward a mixed acid profile and promoted substantial changes in intracellular pool sizes for glycolytic intermediates in cells growing on cellobiose as determined by high-pressure liquid chromatography (HPLC) and nuclear magnetic resonance (NMR). Overall, these data support the genetic evidence of a constriction in cellobiose uptake. Notably, several cell wall precursors accumulated, while other UDP-activated sugar pools were lower, which could reflect rerouting of precursors toward the production of structural or storage polysaccharides. Moreover, cells growing slowly on cellobiose and those lacking\n            <jats:italic>celB<\/jats:italic>\n            were more tolerant to Lcn972 than cellobiose-adapted cells. Thus, downregulation of\n            <jats:italic>celB<\/jats:italic>\n            could help to build up a response against the antimicrobial activity of Lcn972, enhancing self-immunity of the producer cells.\n          <\/jats:p>","DOI":"10.1128\/aem.06107-11","type":"journal-article","created":{"date-parts":[[2011,9,3]],"date-time":"2011-09-03T00:34:12Z","timestamp":1315010052000},"page":"7576-7585","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["The Lcn972 Bacteriocin-Encoding Plasmid pBL1 Impairs Cellobiose Metabolism in Lactococcus lactis"],"prefix":"10.1128","volume":"77","author":[{"given":"Ana B.","family":"Campelo","sequence":"first","affiliation":[{"name":"DairySafe Group. Department of Technology and Biotechnology of Dairy Products, IPLA-CSIC, Carretera de Infiesto s\/n, Apdo. 85, 33300 Villaviciosa, Asturias, Spain"}]},{"given":"Paula","family":"Gaspar","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"given":"Clara","family":"Roces","sequence":"additional","affiliation":[{"name":"DairySafe Group. Department of Technology and Biotechnology of Dairy Products, IPLA-CSIC, Carretera de Infiesto s\/n, Apdo. 85, 33300 Villaviciosa, Asturias, Spain"}]},{"given":"Ana","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"DairySafe Group. Department of Technology and Biotechnology of Dairy Products, IPLA-CSIC, Carretera de Infiesto s\/n, Apdo. 85, 33300 Villaviciosa, Asturias, Spain"}]},{"given":"Jan","family":"Kok","sequence":"additional","affiliation":[{"name":"Department of Genetics, GBB Institute, Rijksuniversiteit Groningen, Centrum voor Levenswetenschappen, Nijenborgh 7, 9747 AG Groningen, The Netherlands"}]},{"given":"Oscar P.","family":"Kuipers","sequence":"additional","affiliation":[{"name":"Department of Genetics, GBB Institute, Rijksuniversiteit Groningen, Centrum voor Levenswetenschappen, Nijenborgh 7, 9747 AG Groningen, The Netherlands"}]},{"given":"Ana Rute","family":"Neves","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"given":"Beatriz","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"DairySafe Group. 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