{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T19:14:15Z","timestamp":1774034055360,"version":"3.50.1"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"14","license":[{"start":{"date-parts":[[2012,7,15]],"date-time":"2012-07-15T00:00:00Z","timestamp":1342310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In general, cellulases and hemicellulases are modular enzymes in which the catalytic domain is appended to one or more noncatalytic carbohydrate binding modules (CBMs). CBMs, by concentrating the parental enzyme at their target polysaccharide, increase the capacity of the catalytic module to bind the substrate, leading to a potentiation in catalysis.\n            <jats:named-content content-type=\"genus-species\">Clostridium thermocellum<\/jats:named-content>\n            hypothetical protein Cthe_0821, defined here as\n            <jats:named-content content-type=\"genus-species\">C. thermocellum<\/jats:named-content>\n            Man5A, is a modular protein comprising an N-terminal signal peptide, a family 5 glycoside hydrolase (GH5) catalytic module, a family 32 CBM (CBM32), and a C-terminal type I dockerin module. Recent proteomic studies revealed that Cthe_0821 is one of the major cellulosomal enzymes when\n            <jats:named-content content-type=\"genus-species\">C. thermocellum<\/jats:named-content>\n            is cultured on cellulose. Here we show that the GH5 catalytic module of Cthe_0821 displays endomannanase activity.\n            <jats:named-content content-type=\"genus-species\">C. thermocellum<\/jats:named-content>\n            Man5A hydrolyzes soluble konjac glucomannan, soluble carob galactomannan, and insoluble ivory nut mannan but does not attack the highly galactosylated mannan from guar gum, suggesting that the enzyme prefers unsubstituted \u03b2-1,4-mannoside linkages. The CBM32 of\n            <jats:named-content content-type=\"genus-species\">C. thermocellum<\/jats:named-content>\n            Man5A displays a preference for the nonreducing ends of mannooligosaccharides, although the protein module exhibits measurable affinity for the termini of \u03b2-1,4-linked glucooligosaccharides such as cellobiose. CBM32 potentiates the activity of\n            <jats:named-content content-type=\"genus-species\">C. thermocellum<\/jats:named-content>\n            Man5A against insoluble mannans but has no significant effect on the capacity of the enzyme to hydrolyze soluble galactomannans and glucomannans. The product profile of\n            <jats:named-content content-type=\"genus-species\">C. thermocellum<\/jats:named-content>\n            Man5A is affected by the presence of CBM32.\n          <\/jats:p>","DOI":"10.1128\/aem.07457-11","type":"journal-article","created":{"date-parts":[[2012,5,5]],"date-time":"2012-05-05T09:51:36Z","timestamp":1336211496000},"page":"4781-4787","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Influence of a Mannan Binding Family 32 Carbohydrate Binding Module on the Activity of the Appended Mannanase"],"prefix":"10.1128","volume":"78","author":[{"given":"Kimiya","family":"Mizutani","sequence":"first","affiliation":[{"name":"Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University, Tsu, Japan"}]},{"given":"V\u00e2nia O.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"CIISA-Faculdade de Medicina Veterin\u00e1ria, Universidade T\u00e9cnica de Lisboa, Lisbon, Portugal"},{"name":"NZYTech Lda, Lisbon, Portugal"}]},{"given":"Shuichi","family":"Karita","sequence":"additional","affiliation":[{"name":"Graduate School of Regional Innovation Studies. Mie University, Tsu, Japan"}]},{"given":"Ana S.","family":"Lu\u00eds","sequence":"additional","affiliation":[{"name":"CIISA-Faculdade de Medicina Veterin\u00e1ria, Universidade T\u00e9cnica de Lisboa, Lisbon, Portugal"}]},{"given":"Makiko","family":"Sakka","sequence":"additional","affiliation":[{"name":"Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University, Tsu, Japan"}]},{"given":"Tetsuya","family":"Kimura","sequence":"additional","affiliation":[{"name":"Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University, Tsu, Japan"}]},{"given":"Adam","family":"Jackson","sequence":"additional","affiliation":[{"name":"Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom"}]},{"given":"Xiaoyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom"}]},{"given":"Carlos M. G. A.","family":"Fontes","sequence":"additional","affiliation":[{"name":"CIISA-Faculdade de Medicina Veterin\u00e1ria, Universidade T\u00e9cnica de Lisboa, Lisbon, Portugal"},{"name":"NZYTech Lda, Lisbon, Portugal"}]},{"given":"Harry J.","family":"Gilbert","sequence":"additional","affiliation":[{"name":"Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom"}]},{"given":"Kazuo","family":"Sakka","sequence":"additional","affiliation":[{"name":"Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University, Tsu, Japan"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.01.030"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.2.504-512.2003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/tcr.20160"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1039\/JR9310001456"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn663"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf011399t"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M606126200"},{"key":"e_1_3_2_9_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-091208-085603"},{"key":"e_1_3_2_11_2","first-page":"153","volume-title":"Laboratory handbook of paper and thin-layer chromatography","author":"Gasparic J","year":"1978","unstructured":"GasparicJ ChuracekJ. 1978. 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