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Several members of the Sap family were claimed to play a significant role in the progression of candidiasis established by the hematogenous route. This assumption was based on the observed attenuated virulence of\n            <jats:italic>sap<\/jats:italic>\n            -null mutant strains. However, the exclusive contribution of\n            <jats:italic>SAP<\/jats:italic>\n            genes to their attenuated phenotype was not unequivocally confirmed, as the Ura status of these mutant strains could also have contributed to the attenuation. In this study, we have reassessed the importance of\n            <jats:italic>SAP1<\/jats:italic>\n            to\n            <jats:italic>SAP6<\/jats:italic>\n            in a murine model of hematogenously disseminated candidiasis using\n            <jats:italic>sap<\/jats:italic>\n            -null mutant strains not affected in their\n            <jats:italic>URA3<\/jats:italic>\n            gene expression and compared their virulence phenotypes with those of Ura-blaster\n            <jats:italic>sap<\/jats:italic>\n            mutants. The median survival time of BALB\/c mice intravenously infected with a mutant strain lacking\n            <jats:italic>SAP1<\/jats:italic>\n            to\n            <jats:italic>SAP3<\/jats:italic>\n            was equivalent to that of mice infected with wild-type strain SC5314, while those infected with mutant strains lacking\n            <jats:italic>SAP5<\/jats:italic>\n            showed slightly extended survival times. Nevertheless, no differences could be observed between the wild type and a \u0394\n            <jats:italic>sap456<\/jats:italic>\n            mutant in their abilities to invade mouse kidneys. Likewise, a deficiency in\n            <jats:italic>SAP4<\/jats:italic>\n            to\n            <jats:italic>SAP6<\/jats:italic>\n            had no noticeable impact on the immune response elicited in the spleens and kidneys of\n            <jats:italic>C. albicans<\/jats:italic>\n            -infected mice. These results contrast with the behavior of equivalent Ura-blaster mutants, which presented a significant reduction in virulence. Our results suggest that Sap1 to Sap6 do not play a significant role in\n            <jats:italic>C. albicans<\/jats:italic>\n            virulence in a murine model of hematogenously disseminated candidiasis and that, in this model, Sap1 to Sap3 are not necessary for successful\n            <jats:italic>C. albicans<\/jats:italic>\n            infection.\n          <\/jats:p>","DOI":"10.1128\/iai.00248-10","type":"journal-article","created":{"date-parts":[[2010,8,3]],"date-time":"2010-08-03T02:16:02Z","timestamp":1280801762000},"page":"4839-4849","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Limited Role of Secreted Aspartyl Proteinases Sap1 to Sap6 in\n            <i>Candida albicans<\/i>\n            Virulence and Host Immune Response in Murine Hematogenously Disseminated Candidiasis"],"prefix":"10.1128","volume":"78","author":[{"given":"Alexandra","family":"Correia","sequence":"first","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, 4710-057 Braga, Portugal"}]},{"given":"Ulrich","family":"Lermann","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Molekulare Infektionsbiologie, Universita\u0308t Wu\u0308rzburg, Josef Schneider Str. 2, D-97080 Wu\u0308rzburg, Germany"}]},{"given":"Luzia","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Unidade Multidisciplinar de Investigac\u0327a\u0303o Biome\u0301dica (UMIB), Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003 Porto, Portugal"},{"name":"Instituto de Cie\u0302ncias Biome\u0301dicas de Abel Salazar (ICBAS), Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003 Porto, Portugal"}]},{"given":"Filipe","family":"Cerca","sequence":"additional","affiliation":[{"name":"Instituto de Cie\u0302ncias Biome\u0301dicas de Abel Salazar (ICBAS), Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003 Porto, Portugal"},{"name":"Instituto de Biologia Molecular e Celular (IBMC), Rua Campo Alegre, 4099-003 Porto, Portugal"}]},{"given":"Sofia","family":"Botelho","sequence":"additional","affiliation":[{"name":"Instituto de Cie\u0302ncias Biome\u0301dicas de Abel Salazar (ICBAS), Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003 Porto, Portugal"}]},{"given":"Rui M.","family":"Gil da Costa","sequence":"additional","affiliation":[{"name":"Instituto de Cie\u0302ncias Biome\u0301dicas de Abel Salazar (ICBAS), Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003 Porto, Portugal"}]},{"given":"Paula","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, 4710-057 Braga, Portugal"}]},{"given":"Fa\u0301tima","family":"Ga\u0308rtner","sequence":"additional","affiliation":[{"name":"Instituto de Cie\u0302ncias Biome\u0301dicas de Abel Salazar (ICBAS), Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003 Porto, Portugal"}]},{"given":"Joachim","family":"Morschha\u0308user","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Molekulare Infektionsbiologie, Universita\u0308t Wu\u0308rzburg, Josef Schneider Str. 2, D-97080 Wu\u0308rzburg, Germany"}]},{"given":"Manuel","family":"Vilanova","sequence":"additional","affiliation":[{"name":"Instituto de Cie\u0302ncias Biome\u0301dicas de Abel Salazar (ICBAS), Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003 Porto, Portugal"},{"name":"Instituto de Biologia Molecular e Celular (IBMC), Rua Campo Alegre, 4099-003 Porto, Portugal"}]},{"given":"Ce\u0301lia","family":"Pais","sequence":"additional","affiliation":[{"name":"Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, 4710-057 Braga, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M509297200"},{"key":"e_1_3_2_3_2","first-page":"3334","volume":"13","year":"2008","unstructured":"Ashman, R. 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