{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T13:34:45Z","timestamp":1773149685576,"version":"3.50.1"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            One of the most crucial events during infection with the dimorphic fungus\n            <jats:italic>Paracoccidioides brasiliensis<\/jats:italic>\n            is adhesion to pulmonary epithelial cells, a pivotal step in the establishment of disease. In this study, we have evaluated the relevance of a 32-kDa protein, a putative adhesion member of the haloacid dehalogenase (HAD) superfamily of hydrolases, in the virulence of this fungus. Protein sequence analyses have supported the inclusion of PbHad32p as a hydrolase and have revealed a conserved protein only among fungal dimorphic and filamentous pathogens that are closely phylogenetically related. To evaluate its role during the host-pathogen interaction, we have generated mitotically stable\n            <jats:italic>P. brasiliensis HAD32<\/jats:italic>\n            (\n            <jats:italic>PbHAD32<\/jats:italic>\n            ) antisense RNA (aRNA) strains with consistently reduced gene expression. Knockdown of\n            <jats:italic>PbHAD32<\/jats:italic>\n            did not alter cell vitality or viability but induced morphological alterations in yeast cells. Moreover, yeast cells with reduced\n            <jats:italic>PbHAD32<\/jats:italic>\n            expression were significantly affected in their capacity to adhere to human epithelial cells and presented decreased virulence in a mouse model of infection. These data support the hypothesis that PbHad32p binds to extracellular matrix (ECM) proteins and modulates the initial immune response for evasion of host defenses. Our findings point to\n            <jats:italic>PbHAD32<\/jats:italic>\n            as a novel virulence factor active during the initial interaction with host cells in\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.00692-10","type":"journal-article","created":{"date-parts":[[2010,9,28]],"date-time":"2010-09-28T04:24:12Z","timestamp":1285647852000},"page":"5280-5286","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["A 32-Kilodalton Hydrolase Plays an Important Role in\n            <i>Paracoccidioides brasiliensis<\/i>\n            Adherence to Host Cells and Influences Pathogenicity"],"prefix":"10.1128","volume":"78","author":[{"given":"Orville","family":"Herna\u0301ndez","sequence":"first","affiliation":[{"name":"Instituto de Biologi\u0301a, Universidad de Antioquia, Medelli\u0301n, Colombia"},{"name":"Cellular and Molecular Biology Unit, Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia"}]},{"given":"Agostinho J.","family":"Almeida","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal"}]},{"given":"Angel","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Medical and Experimental Mycology Group, Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia"},{"name":"Escuela de Microbiologi\u0301a, Universidad de Antioquia, Medelli\u0301n, Colombia"}]},{"given":"Ana Maria","family":"Garcia","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Biology Unit, Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia"}]},{"given":"Diana","family":"Tamayo","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Biology Unit, Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia"}]},{"given":"Luz Elena","family":"Cano","sequence":"additional","affiliation":[{"name":"Medical and Experimental Mycology Group, Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia"},{"name":"Escuela de Microbiologi\u0301a, Universidad de Antioquia, Medelli\u0301n, Colombia"}]},{"given":"Angela","family":"Restrepo","sequence":"additional","affiliation":[{"name":"Medical and Experimental Mycology Group, Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia"}]},{"given":"Juan G.","family":"McEwen","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Biology Unit, Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia"},{"name":"Facultad de Medicina, Universidad de Antioquia, Medelli\u0301n, Colombia"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2007.04.004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2009.08.004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47127-0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.382-389.2006"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.256.5061.1324"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.6.2.89"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00683967"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-272"},{"key":"e_1_3_2_10_2","first-page":"63","volume":"55","year":"1995","unstructured":"den Dulk-Ras, A., and P. 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