{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T03:16:28Z","timestamp":1776395788959,"version":"3.51.2"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>\n            serovar Typhimurium is a bacterial pathogen causing gastroenteritis in humans and a typhoid-like systemic disease in mice.\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium virulence is related to its capacity to multiply intracellularly within a membrane-bound compartment, the\n            <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n            -containing vacuole (SCV), and depends on type III secretion systems that deliver bacterial effector proteins into host cells. Here, we analyzed the cellular function of the\n            <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n            effector SteA. We show that, compared to cells infected by wild-type\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium, cells infected by \u0394\n            <jats:italic>steA<\/jats:italic>\n            mutant bacteria displayed fewer\n            <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n            -induced filaments (SIFs), an increased clustering of SCVs, and morphologically abnormal vacuoles containing more than one bacterium. The increased clustering of SCVs and the appearance of vacuoles containing more than one bacterium were suppressed by inhibition of the activity of the microtubule motor dynein or kinesin-1. Clustering and positioning of SCVs are controlled by the effectors SseF and SseG, possibly by helping to maintain a balanced activity of microtubule motors on the bacterial vacuoles. Deletion of\n            <jats:italic>steA<\/jats:italic>\n            in\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium \u0394\n            <jats:italic>sseF<\/jats:italic>\n            or \u0394\n            <jats:italic>sseG<\/jats:italic>\n            mutants revealed that SteA contributes to the characteristic scattered distribution of \u0394\n            <jats:italic>sseF<\/jats:italic>\n            or \u0394\n            <jats:italic>sseG<\/jats:italic>\n            mutant SCVs in infected cells. Overall, this shows that SteA participates in the control of SCV membrane dynamics. Moreover, it indicates that SteA is functionally linked to SseF and SseG and suggests that it might contribute directly or indirectly to the regulation of microtubule motors on the bacterial vacuoles.\n          <\/jats:p>","DOI":"10.1128\/iai.01385-13","type":"journal-article","created":{"date-parts":[[2014,4,29]],"date-time":"2014-04-29T06:34:38Z","timestamp":1398753278000},"page":"2923-2934","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["The Salmonella Effector SteA Contributes to the Control of Membrane Dynamics of Salmonella-Containing Vacuoles"],"prefix":"10.1128","volume":"82","author":[{"given":"Lia","family":"Domingues","sequence":"first","affiliation":[{"name":"Infection Biology Laboratory, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal"},{"name":"Centro de Recursos Microbiol\u00f3gicos (CREM), Departamento de Ci\u00eancias da Vida, Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal"}]},{"given":"David W.","family":"Holden","sequence":"additional","affiliation":[{"name":"MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, United Kingdom"}]},{"given":"Lu\u00eds Jaime","family":"Mota","sequence":"additional","affiliation":[{"name":"Infection Biology Laboratory, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal"},{"name":"Centro de Recursos Microbiol\u00f3gicos (CREM), Departamento de Ci\u00eancias da Vida, Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, Caparica, 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