{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T14:16:21Z","timestamp":1771078581826,"version":"3.50.1"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2012,4,1]],"date-time":"2012-04-01T00:00:00Z","timestamp":1333238400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We investigated the outcome of the interaction of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Cryptococcus neoformans<\/jats:named-content>\n            with murine macrophages using laser scanning cytometry (LSC). Previous results in our lab had shown that phagocytosis of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. neoformans<\/jats:named-content>\n            promoted cell cycle progression. LSC allowed us to simultaneously measure the phagocytic index, macrophage DNA content, and 5-ethynyl-2\u2032-deoxyuridine (EdU) incorporation such that it was possible to study host cell division as a function of phagocytosis. LSC proved to be a robust, reliable, and high-throughput method for quantifying phagocytosis. Phagocytosis of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. neoformans<\/jats:named-content>\n            promoted cell cycle progression, but infected macrophages were significantly less likely to complete mitosis. Hence, we report a new cytotoxic effect associated with intracellular\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. neoformans<\/jats:named-content>\n            residence that manifested itself in impaired cell cycle completion as a consequence of a block in the G\n            <jats:sub>2<\/jats:sub>\n            \/M stage of the mitotic cell cycle. Cell cycle arrest was not due to increased cell membrane permeability or DNA damage. We investigated alveolar macrophage replication\n            <jats:italic>in vivo<\/jats:italic>\n            and demonstrated that these cells are capable of low levels of cell division in the presence or absence of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. neoformans<\/jats:named-content>\n            infection. In summary, we simultaneously studied phagocytosis, the cell cycle state of the host cell and pathogen-mediated cytotoxicity, and our results demonstrate a new cytotoxic effect of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. neoformans<\/jats:named-content>\n            infection on murine macrophages: fungus-induced cell cycle arrest. Finally, we provide evidence for alveolar macrophage proliferation\n            <jats:italic>in vivo<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.06332-11","type":"journal-article","created":{"date-parts":[[2012,1,18]],"date-time":"2012-01-18T05:17:41Z","timestamp":1326863861000},"page":"1467-1478","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Analysis of Cell Cycle and Replication of Mouse Macrophages after\n            <i>In Vivo<\/i>\n            and\n            <i>In Vitro<\/i>\n            Cryptococcus neoformans Infection Using Laser Scanning Cytometry"],"prefix":"10.1128","volume":"80","author":[{"given":"Carolina","family":"Coelho","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York, USA"},{"name":"Ph.D. Programme in Experimental Biology and Biomedicine, Centre for Neuroscience and Cell Biology of Coimbra and Institute of Microbiology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal"}]},{"given":"Lydia","family":"Tesfa","sequence":"additional","affiliation":[{"name":"Flow Cytometry Core Facility, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York, USA"}]},{"given":"Jinghang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Flow Cytometry Core Facility, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York, USA"}]},{"given":"Johanna","family":"Rivera","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York, USA"}]},{"given":"Teresa","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Ph.D. Programme in Experimental Biology and Biomedicine, Centre for Neuroscience and Cell Biology of Coimbra and Institute of Microbiology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal"}]},{"given":"Arturo","family":"Casadevall","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York, USA"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2887"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-51"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2006.09.061"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111443"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.6.1437"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2009.01.011"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05230-0"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.21048"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201141817"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4225-4237.2000"},{"key":"e_1_3_3_12_2","first-page":"161","article-title":"Laser scanning cytometry and its applications: a pioneering technology in the field of quantitative imaging cytometry","volume":"102","author":"Henriksen M","year":"2011","unstructured":"HenriksenMMillerBNewmarkJAl-KofahiYHoldenE. 2011. 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