{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T11:04:59Z","timestamp":1760267099167},"reference-count":59,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Analysis of sequenced bacterial genomes revealed that the genomes encode more than 30% hypothetical and conserved hypothetical proteins of unknown function. Among proteins of unknown function that are conserved in anaerobes, some might be determinants of the anaerobic way of life. This study focuses on two divergent clusters specifically found in anaerobic microorganisms and mainly composed of genes encoding conserved hypothetical proteins. We show that the two gene clusters\n            <jats:italic>DVU2103-DVU2104-DVU2105<\/jats:italic>\n            (\n            <jats:italic>orp2<\/jats:italic>\n            ) and\n            <jats:italic>DVU2107-DVU2108-DVU2109<\/jats:italic>\n            (\n            <jats:italic>orp1<\/jats:italic>\n            ) form two divergent operons transcribed by the \u03c3\n            <jats:sup>54<\/jats:sup>\n            -RNA polymerase. We further demonstrate that the \u03c3\n            <jats:sup>54<\/jats:sup>\n            -dependent transcriptional regulator DVU2106, located between\n            <jats:italic>orp1<\/jats:italic>\n            and\n            <jats:italic>orp2<\/jats:italic>\n            , collaborates with \u03c3\n            <jats:sup>54<\/jats:sup>\n            -RNA polymerase to orchestrate the simultaneous expression of the divergent\n            <jats:italic>orp<\/jats:italic>\n            operons. DVU2106, whose structural gene is transcribed by the \u03c3\n            <jats:sup>70<\/jats:sup>\n            -RNA polymerase, negatively retrocontrols its own expression. By using an endogenous pulldown strategy, we identify a physiological complex composed of DVU2103, DVU2104, DVU2105, DVU2108, and DVU2109. Interestingly, inactivation of DVU2106, which is required for\n            <jats:italic>orp<\/jats:italic>\n            operon transcription, induces morphological defects that are likely linked to the absence of the ORP complex. A putative role of the ORP proteins in positioning the septum during cell division is discussed.\n          <\/jats:p>","DOI":"10.1128\/jb.00044-11","type":"journal-article","created":{"date-parts":[[2011,4,30]],"date-time":"2011-04-30T02:17:29Z","timestamp":1304129849000},"page":"3207-3219","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["The Anaerobe-Specific Orange Protein Complex of Desulfovibrio vulgaris Hildenborough Is Encoded by Two Divergent Operons Coregulated by \u03c3\n            <sup>54<\/sup>\n            and a Cognate Transcriptional Regulator"],"prefix":"10.1128","volume":"193","author":[{"given":"Anouchka","family":"Fi\u00e9vet","sequence":"first","affiliation":[{"name":"Laboratoire Interactions et Modulateurs de R\u00e9ponses, Institut de Microbiologie de la M\u00e9diterran\u00e9e, CNRS 13402 Marseille Cedex 20, France"}]},{"given":"Laetitia","family":"My","sequence":"additional","affiliation":[{"name":"Laboratoire Interactions et Modulateurs de R\u00e9ponses, Institut de Microbiologie de la M\u00e9diterran\u00e9e, CNRS 13402 Marseille Cedex 20, France"}]},{"given":"Eric","family":"Cascales","sequence":"additional","affiliation":[{"name":"Laboratoire d'Ing\u00e9nierie des Syst\u00e8mes Macromol\u00e9culaires, Institut de Microbiologie de la M\u00e9diterran\u00e9e, CNRS 13402 Marseille Cedex 20, France"}]},{"given":"Mireille","family":"Ansaldi","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Bact\u00e9rienne, Institut de Microbiologie de la M\u00e9diterran\u00e9e, CNRS 13402 Marseille Cedex 20, France"}]},{"given":"Sofia R.","family":"Pauleta","sequence":"additional","affiliation":[{"name":"Requimente, Departamento de Qimica, Centro de Qimica Fina e Biotecnologia, Faculdade de Ciencas e Tecnologica, Universidade Nova de Lisboa, Caparica 2829-516, Portugal"}]},{"given":"Isabel","family":"Moura","sequence":"additional","affiliation":[{"name":"Requimente, Departamento de Qimica, Centro de Qimica Fina e Biotecnologia, Faculdade de Ciencas e Tecnologica, Universidade Nova de Lisboa, Caparica 2829-516, Portugal"}]},{"given":"Zorah","family":"Dermoun","sequence":"additional","affiliation":[{"name":"Laboratoire Interactions et Modulateurs de R\u00e9ponses, Institut de Microbiologie de la M\u00e9diterran\u00e9e, CNRS 13402 Marseille Cedex 20, France"}]},{"given":"Christophe S.","family":"Bernard","sequence":"additional","affiliation":[{"name":"Laboratoire d'Ing\u00e9nierie des Syst\u00e8mes Macromol\u00e9culaires, Institut de Microbiologie de la M\u00e9diterran\u00e9e, CNRS 13402 Marseille Cedex 20, France"}]},{"given":"Alain","family":"Dolla","sequence":"additional","affiliation":[{"name":"Laboratoire Interactions et Modulateurs de R\u00e9ponses, Institut de Microbiologie de la M\u00e9diterran\u00e9e, CNRS 13402 Marseille Cedex 20, France"}]},{"given":"Corinne","family":"Aubert","sequence":"additional","affiliation":[{"name":"Laboratoire Interactions et Modulateurs de R\u00e9ponses, Institut de Microbiologie de la M\u00e9diterran\u00e9e, CNRS 13402 Marseille Cedex 20, France"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.4.961-966.2000"},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1111\/j.1365-2958.2009.07028.x","article-title":"The SciZ protein anchors the enteroaggregative Escherichia coli type VI secretion system to the cell wall","volume":"75","author":"Aschtgen M. 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