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The corresponding gene encoded a putative glycosyltransferase that was part of an operon-like structure which also contained the genes for glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP), the enzymes that lead to the synthesis of GG through the formation of glucosyl-3-phosphoglycerate. The putative glucosyltransferase gene was expressed in<jats:italic>Escherichia coli<\/jats:italic>, and the recombinant product catalyzed the synthesis of GG in one step from ADP-glucose and<jats:sc>d<\/jats:sc>-glycerate, with<jats:italic>K<jats:sub>m<\/jats:sub><\/jats:italic>values at 70\u00b0C of 1.5 and 2.2 mM, respectively. This glucosylglycerate synthase (Ggs) was also able to use GDP- and UDP-glucose as donors to form GG, but the efficiencies were lower. Maximal activity was observed at temperatures between 80 and 85\u00b0C, and Mg<jats:sup>2+<\/jats:sup>or Ca<jats:sup>2+<\/jats:sup>was required for catalysis. Ggs activity was maximal and remained nearly constant at pH values between 5.5 and pH 8.0, and the half-lives for inactivation were 74 h at 85\u00b0C and 8 min at 100\u00b0C. This is the first report of an enzyme catalyzing the synthesis of GG in one step and of the existence of two pathways for GG synthesis in the same organism.<\/jats:p>","DOI":"10.1128\/jb.00075-07","type":"journal-article","created":{"date-parts":[[2007,3,16]],"date-time":"2007-03-16T21:21:07Z","timestamp":1174080067000},"page":"4014-4019","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Single-Step Pathway for Synthesis of Glucosylglycerate in<i>Persephonella marina<\/i>"],"prefix":"10.1128","volume":"189","author":[{"given":"Chantal","family":"Fernandes","sequence":"first","affiliation":[{"name":"Centro de Neurocie\u0302ncias e Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"Nuno","family":"Empadinhas","sequence":"additional","affiliation":[{"name":"Centro de Neurocie\u0302ncias e Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"Milton S.","family":"da Costa","sequence":"additional","affiliation":[{"name":"Departamento de Bioqui\u0301mica, Universidade de Coimbra, 3001-401 Coimbra, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"9892","DOI":"10.1074\/jbc.M312186200","volume":"279","year":"2004","unstructured":"Borges, N., J. 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