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Activation of \u03c3\n            <jats:sup>G<\/jats:sup>\n            also requires expression of\n            <jats:italic>spoIIIJ<\/jats:italic>\n            , coding for a membrane protein translocase of the YidC\/Oxa1p\/Alb3 family, and of the mother cell-specific\n            <jats:italic>spoIIIA<\/jats:italic>\n            operon. Here we present genetic and biochemical evidence indicating that SpoIIIAE, the product of one of the\n            <jats:italic>spoIIIA<\/jats:italic>\n            cistrons, and SpoIIIJ interact in the membrane, thereby linking the function of the\n            <jats:italic>spoIIIJ<\/jats:italic>\n            and\n            <jats:italic>spoIIIA<\/jats:italic>\n            loci in the activation of \u03c3\n            <jats:sup>G<\/jats:sup>\n            . We also show that SpoIIIAE has a functional Sec-type signal peptide, which is cleaved during sporulation. Furthermore, mutations that reduce or eliminate processing of the SpoIIIAE signal peptide arrest sporulation following engulfment completion and prevent activation of \u03c3\n            <jats:sup>G<\/jats:sup>\n            . SpoIIIJ-type proteins can function in cooperation with or independently of the Sec system. In one model, SpoIIIJ interacts with SpoIIIAE in the context of the Sec translocon to promote its correct localization and\/or topology in the membrane, so that it can signal the activation of \u03c3\n            <jats:sup>G<\/jats:sup>\n            following engulfment completion.\n          <\/jats:p>","DOI":"10.1128\/jb.00715-08","type":"journal-article","created":{"date-parts":[[2008,9,27]],"date-time":"2008-09-27T00:34:42Z","timestamp":1222475682000},"page":"7786-7796","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Processing of a Membrane Protein Required for Cell-to-Cell Signaling during Endospore Formation in\n            <i>Bacillus subtilis<\/i>"],"prefix":"10.1128","volume":"190","author":[{"given":"Mo\u0301nica","family":"Serrano","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Avenida da Repu\u0301blica, Apartado 127, 2781-901 Oeiras Codex, Portugal"}]},{"given":"Filipe","family":"Vieira","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Avenida da Repu\u0301blica, Apartado 127, 2781-901 Oeiras Codex, Portugal"}]},{"suffix":"Jr.","given":"Charles P.","family":"Moran","sequence":"additional","affiliation":[{"name":"Emory University School of Medicine, Department of Microbiology and Immunology, Atlanta, Georgia 30322"}]},{"given":"Adriano O.","family":"Henriques","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Avenida da Repu\u0301blica, Apartado 127, 2781-901 Oeiras Codex, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.05.028"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"2916","DOI":"10.1101\/gad.1252704","volume":"18","year":"2004","unstructured":"Blaylock, B., X. 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