{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T06:09:13Z","timestamp":1774332553221,"version":"3.50.1"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2010,10,15]],"date-time":"2010-10-15T00:00:00Z","timestamp":1287100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2010,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Bacillus subtilis<\/jats:italic>\n            is able to utilize arabinopolysaccharides derived from plant biomass. Here, by combining genetic and physiological analyses we characterize the AraNPQ importer and identify primary and secondary transporters of\n            <jats:italic>B. subtilis<\/jats:italic>\n            involved in the uptake of arabinosaccharides. We show that the ABC-type importer AraNPQ is involved in the uptake of \u03b1-1,5-arabinooligosaccharides, at least up to four\n            <jats:sc>l<\/jats:sc>\n            -arabinosyl units. Although this system is the key transporter for \u03b1-1,5-arabinotriose and \u03b1-1,5-arabinotetraose, the results indicate that \u03b1-1,5-arabinobiose also is translocated by the secondary transporter AraE. This broad-specificity proton symporter is the major transporter for arabinose and also is accountable for the uptake of xylose and galactose. In addition, MsmX is shown to be the ATPase that energizes the incomplete AraNPQ importer. Furthermore, the results suggest the existence of at least one more unidentified MsmX-dependent ABC importer responsible for the uptake of nonlinear \u03b1-1,2- and \u03b1-1,3-arabinooligosaccharides. This study assigns MsmX as a multipurpose\n            <jats:italic>B. subtilis<\/jats:italic>\n            ATPase required to energize different saccharide transporters, the arabinooligosaccharide-specific AraNPQ-MsmX system, a putative MsmX-dependent ABC transporter specific for nonlinear arabinooligosaccharides, and the previously characterized maltodextrin-specific MdxEFG-MsmX system.\n          <\/jats:p>","DOI":"10.1128\/jb.00832-10","type":"journal-article","created":{"date-parts":[[2010,8,7]],"date-time":"2010-08-07T02:22:22Z","timestamp":1281147742000},"page":"5312-5318","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["A Multitask ATPase Serving Different ABC-Type Sugar Importers in\n            <i>Bacillus subtilis<\/i>"],"prefix":"10.1128","volume":"192","author":[{"given":"Ma\u0301rio Jose\u0301","family":"Ferreira","sequence":"first","affiliation":[{"name":"Centro de Recursos Microbiolo\u0301gicos, Departamento de Cie\u0302ncias da Vida, Faculdade de Cie\u0302ncias e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, 2829-516 Caparica, Portugal"},{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Avenida de Repu\u0301blica-EAN, 2780-157 Oeiras, Portugal"}]},{"given":"Isabel de","family":"Sa\u0301-Nogueira","sequence":"additional","affiliation":[{"name":"Centro de Recursos Microbiolo\u0301gicos, Departamento de Cie\u0302ncias da Vida, Faculdade de Cie\u0302ncias e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, 2829-516 Caparica, Portugal"},{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Avenida de Repu\u0301blica-EAN, 2780-157 Oeiras, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.81.5.741-746.1961"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.11.6887-6891.2004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00031-07"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.7.3966-3973.1990"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1046\/j.1365-2958.1998.00915.x","volume":"29","year":"1998","unstructured":"Ehrmann, M., R. 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Microbiology 154 : 2719-2729.","journal-title":"Microbiology"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00162-08"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.12.3250-3252.1998"},{"key":"e_1_3_2_14_2","unstructured":"Experiments in molecular genetics. 1972"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.sbi.2008.09.007","volume":"18","year":"2008","unstructured":"Oldham, M. L., A. L. Davidson, and J. Chen. 2008. Structural insights into ABC transporter mechanism. Curr. Opin. Struct. Biol. 18 : 726-733.","journal-title":"Curr. Opin. Struct. Biol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1038\/nature06264","volume":"450","year":"2007","unstructured":"Oldham, M. L., D. Khare, F. A. Quiocho, A. L. Davidson, and J. Chen. 2007. Crystal structure of a catalytic intermediate of the maltose transporter. 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