{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:07:33Z","timestamp":1761894453295,"version":"3.32.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The pathway for the synthesis of glucosylglycerate (GG) in the thermophilic bacterium<jats:italic>Persephonella marina<\/jats:italic>is proposed based on the activities of recombinant glucosyl-3-phosphoglycerate (GPG) synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP). The sequences of<jats:italic>gpgS<\/jats:italic>and<jats:italic>gpgP<\/jats:italic>from the cold-adapted bacterium<jats:italic>Methanococcoides burtonii<\/jats:italic>were used to identify the homologues in the genome of<jats:italic>P. marina<\/jats:italic>, which were separately cloned and overexpressed as His-tagged proteins in<jats:italic>Escherichia coli<\/jats:italic>. The recombinant GpgS protein of<jats:italic>P. marina<\/jats:italic>, unlike the homologue from<jats:italic>M. burtonii<\/jats:italic>, which was specific for GDP-glucose, catalyzed the synthesis of GPG from UDP-glucose, GDP-glucose, ADP-glucose, and TDP-glucose (in order of decreasing efficiency) and from<jats:sc>d<\/jats:sc>-3-phosphoglycerate, with maximal activity at 90\u00b0C. The recombinant GpgP protein, like the<jats:italic>M. burtonii<\/jats:italic>homologue, dephosphorylated GPG and mannosyl-3-phosphoglycerate (MPG) to GG and mannosylglycerate, respectively, yet at high temperatures the hydrolysis of GPG was more efficient than that of MPG. Gel filtration indicates that GpgS is a dimeric protein, while GpgP is monomeric. This is the first characterization of genes and enzymes for the synthesis of GG in a thermophile.<\/jats:p>","DOI":"10.1128\/jb.00841-06","type":"journal-article","created":{"date-parts":[[2006,12,23]],"date-time":"2006-12-23T01:34:25Z","timestamp":1166837665000},"page":"1648-1654","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Glucosylglycerate Biosynthesis in the Deepest Lineage of the<i>Bacteria<\/i>: Characterization of the Thermophilic Proteins GpgS and GpgP from<i>Persephonella marina<\/i>"],"prefix":"10.1128","volume":"189","author":[{"given":"Joana","family":"Costa","sequence":"first","affiliation":[{"name":"Centro de Neurocie\u0302ncias e Biologia Celular, Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"Nuno","family":"Empadinhas","sequence":"additional","affiliation":[{"name":"Centro de Neurocie\u0302ncias e Biologia Celular, Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"Milton S.","family":"da Costa","sequence":"additional","affiliation":[{"name":"Departamento de Bioqui\u0301mica, Universidade de Coimbra, 3001-401 Coimbra, Portugal"}]}],"member":"235","reference":[{"doi-asserted-by":"publisher","key":"e_1_3_2_2_2","DOI":"10.1128\/AEM.71.5.2460-2466.2005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0076-6879(66)08014-5","volume":"8","year":"1966","unstructured":"Ames, B. N. 1966. Assay of inorganic phosphate, total phosphate and phosphatases. Methods Enzymol. 8 : 115-118.","journal-title":"Methods Enzymol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1046\/j.1462-2920.2003.00419.x","volume":"5","year":"2003","unstructured":"Bakermans, C., A. I. Tsapin, V. Souza-Egipsy, D. A. Gilichinsky, and K. H. Nealson. 2003. Reproduction and metabolism at \u221210\u00b0C of bacteria isolated from Siberian permafrost. Environ. Microbiol. 5 : 321-326.","journal-title":"Environ. Microbiol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"30315","DOI":"10.1074\/jbc.M010527200","volume":"276","year":"2001","unstructured":"Battistoni, A., F. Pacello, A. P. Mazzetti, C. Capo, J. S. Kroll, P. R. Langford, A. Sansone, G. Donnarumma, P. Valenti, and G. Rotilio. 2001. A histidine-rich metal binding domain at the N terminus of Cu, Zn-superoxide dismutases from pathogenic bacteria. J. Biol. Chem. 276 : 30315-30325.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"9892","DOI":"10.1074\/jbc.M312186200","volume":"279","year":"2004","unstructured":"Borges, N., J. D. Marugg, N. Empadinhas, M. S. da Costa, and H. Santos. 2004. Specialized roles of the two pathways for the synthesis of mannosylglycerate in osmoadaptation and thermoadaptation of Rhodothermus marinus. J. Biol. Chem. 279 : 9892-9898.","journal-title":"J. Biol. Chem."},{"doi-asserted-by":"publisher","key":"e_1_3_2_7_2","DOI":"10.1128\/AEM.65.3.1222-1227.1999"},{"doi-asserted-by":"publisher","key":"e_1_3_2_8_2","DOI":"10.1016\/0003-2697(76)90527-3"},{"doi-asserted-by":"publisher","key":"e_1_3_2_9_2","DOI":"10.1128\/AEM.65.9.3774-3779.1999"},{"doi-asserted-by":"publisher","key":"e_1_3_2_10_2","DOI":"10.1128\/JB.188.3.1022-1030.2006"},{"doi-asserted-by":"publisher","key":"e_1_3_2_11_2","DOI":"10.1128\/JB.180.24.6776-6779.1998"},{"doi-asserted-by":"publisher","key":"e_1_3_2_12_2","DOI":"10.1128\/jb.111.2.340-342.1972"},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/S0021-9258(18)96874-2","volume":"241","year":"1966","unstructured":"Doughty, C. C., J. A. Hayashi, and H. L. Guenther. 1966. Purification and properties of d-glycerate 3-kinase from Escherichia coli. J. Biol. Chem. 241 : 568-572.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"17R","DOI":"10.1093\/glycob\/cwg047","volume":"13","year":"2003","unstructured":"Elbein, A. D., Y. T. Pan, I. Pastuszak, and D. Carroll. 2003. New insights on trehalose: a multifunctional molecule. Glycobiology 13 : 17R-27R.","journal-title":"Glycobiology"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"43580","DOI":"10.1074\/jbc.M108054200","volume":"276","year":"2001","unstructured":"Empadinhas, N., J. D. Marugg, N. Borges, H. Santos, and M. S. da Costa. 2001. Pathway for the synthesis of mannosylglycerate in the hyperthermophilic archaeon Pyrococcus horikoshii. Biochemical and genetic characterization of key enzymes. J. Biol. Chem. 276 : 43580-43588.","journal-title":"J. Biol. Chem."},{"doi-asserted-by":"publisher","key":"e_1_3_2_16_2","DOI":"10.1128\/AEM.69.6.3272-3279.2003"},{"doi-asserted-by":"publisher","key":"e_1_3_2_17_2","DOI":"10.1128\/JB.186.13.4075-4084.2004"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1042\/BJ20051160","volume":"393","year":"2006","unstructured":"Ge, R., R. M. Watt, X. Sun, J. A. Tanner, Q.-Y. He, J.-D. Huang, and H. Sun. 2006. Expression and characterization of a histidine-rich protein, Hpn: potential for Ni2+ storage in Helicobacter pylori. Biochem. J. 393 : 285-293.","journal-title":"Biochem. J."},{"doi-asserted-by":"publisher","key":"e_1_3_2_19_2","DOI":"10.1128\/jb.170.6.2841-2849.1988"},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1021\/bi00866a011","volume":"5","year":"1966","unstructured":"Good, N. E., G. D. Winget, W. Winter, T. N. Connolly, S. Izawa, and R. M. Singh. 1966. Hydrogen ion buffers for biological research. Biochemistry 5 : 467-477.","journal-title":"Biochemistry"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1099\/00207713-52-4-1349","volume":"52","year":"2002","unstructured":"Gotz, D., A. Banta, T. J. Beveridge, A. I. Rushdi, B. R. Simoneit, and A.-L. Reysenbach. 2002. Persephonella marina gen. nov., sp. nov. and Persephonella guaymasensis sp. nov., two novel, thermophilic, hydrogen-oxidizing microaerophiles from deep-sea hydrothermal vents. Int. J. Syst. Evol. Microbiol. 52 : 1349-1359.","journal-title":"Int. J. Syst. Evol. Microbiol."},{"doi-asserted-by":"publisher","key":"e_1_3_2_22_2","DOI":"10.1128\/AEM.70.11.6535-6541.2004"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1007\/s002840010301","volume":"43","year":"2001","unstructured":"Hagemann, M., U. Effmert, T. Kerstan, A. Schoor, and N. Erdmann. 2001. Biochemical characterization of glucosylglycerol-phosphate synthase of Synechocystis sp. strain PCC 6803: comparison of crude, purified, and recombinant enzymes. Curr. Microbiol. 43 : 278-283.","journal-title":"Curr. Microbiol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1023\/A:1006425214168","volume":"45","year":"2001","unstructured":"Hirani, T. A., I. Suzuki, N. Murata, H. Hayashi, and J. J. Eaton-Rye. 2001. Characterization of a two-component signal transduction system involved in the induction of alkaline phosphatase under phosphate-limiting conditions in Synechocystis sp. PCC 6803. Plant Mol. Biol. 45 : 133-144.","journal-title":"Plant Mol. Biol."},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s002030050685","volume":"171","year":"1999","unstructured":"Jackson, B. E., V. K. Bhupathiraju, R. S. Tanner, C. R. Woese, and M. J. McInerney. 1999. Syntrophus aciditrophicus sp. nov., a new anaerobic bacterium that degrades fatty acids and benzoate in syntrophic association with hydrogen-using microorganisms. Arch. Microbiol. 171 : 107-114.","journal-title":"Arch. Microbiol."},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1046\/j.1529-8817.2003.02192.x","volume":"39","year":"2003","unstructured":"Karsten, U., J. A. West, G. C. Zuccarello, R. Engbrodt, A. Yokoyama, Y. Hara, and J. Brodie. 2003. Low molecular weight carbohydrates of the Bangiophycidae (Rhodophyta). J. Phycol. 39 : 584-589.","journal-title":"J. Phycol."},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S0008-6215(00)85489-0","volume":"73","year":"1979","unstructured":"Kollman, V. H., J. L. Hanners, R. E. London, E. G. Adame, and T. E. Walker. 1979. Photosynthetic preparation and characterization of 13C-labeled carbohydrates in Agmenellum quadruplicatum. Carbohydr. Res. 73 : 193-202.","journal-title":"Carbohydr. Res."},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1093\/bib\/5.2.150","volume":"5","year":"2004","unstructured":"Kumar, S., K. Tamura, and M. Nei. 2004. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief. Bioinform. 5 : 150-163.","journal-title":"Brief. Bioinform."},{"doi-asserted-by":"publisher","key":"e_1_3_2_29_2","DOI":"10.1128\/AEM.00852-06"},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"40534","DOI":"10.1074\/jbc.M306907200","volume":"278","year":"2003","unstructured":"Mana-Capelli, S., A. K. Mandal, and J. M. Arguello. 2003. Archaeoglobus fulgidus CopB is a thermophilic Cu2+-ATPase. J. Biol. Chem. 278 : 40534-40541.","journal-title":"J. Biol. Chem."},{"doi-asserted-by":"publisher","key":"e_1_3_2_31_2","DOI":"10.1128\/jb.178.19.5644-5651.1996"},{"doi-asserted-by":"publisher","key":"e_1_3_2_32_2","DOI":"10.1128\/JB.187.12.4238-4244.2005"},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1099\/00207713-46-4-1088","volume":"46","year":"1996","unstructured":"Rainey, F. A., N. Ward-Rainey, R. M. Kroppenstedt, and E. Stackebrandt. 1996. The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int. J. Syst. Bacteriol. 46 : 1088-1092.","journal-title":"Int. J. Syst. Bacteriol."},{"doi-asserted-by":"publisher","key":"e_1_3_2_34_2","DOI":"10.1128\/aem.58.8.2438-2443.1992"},{"unstructured":"Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory Cold Spring Harbor NY.","key":"e_1_3_2_35_2"},{"key":"e_1_3_2_36_2","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1046\/j.1462-2920.2002.00335.x","volume":"4","year":"2002","unstructured":"Santos, H., and M. S. da Costa. 2002. Compatible solutes of organisms that live in hot saline environments. Environ. Microbiol. 4 : 501-509.","journal-title":"Environ. Microbiol."},{"doi-asserted-by":"publisher","key":"e_1_3_2_37_2","DOI":"10.1128\/JB.185.20.5943-5952.2003"},{"doi-asserted-by":"publisher","key":"e_1_3_2_38_2","DOI":"10.1128\/jb.175.21.6797-6809.1993"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1002\/pro.5560070722","volume":"7","year":"1998","unstructured":"Thaller, M. C., S. Schippa, and G. M. Rossolini. 1998. Conserved sequence motifs among bacterial, eukaryotic, and archaeal phosphatases that define a new phosphohydrolase superfamily. Protein Sci. 7 : 1647-1652.","journal-title":"Protein Sci."},{"key":"e_1_3_2_40_2","first-page":"4876","volume":"24","year":"1997","unstructured":"Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin, and D. G. Higgins. 1997. The ClustalX Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24 : 4876-4882.","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"3307","DOI":"10.1021\/bi052467y","volume":"45","year":"2006","unstructured":"Wang, Y., A. Morgan, K. Stieglitz, B. Stec, B. Thompson, S. Miller, and M. F. Roberts. 2006. The temperature dependence of the inositol monophosphatase K m correlates with accumulation of di-myo-inositol 1,1\u2032-phosphate in Archaeoglobus fulgidus. Biochemistry 45 : 3307-3314.","journal-title":"Biochemistry"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00841-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.00841-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,12]],"date-time":"2025-01-12T18:19:09Z","timestamp":1736705949000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.00841-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3]]},"references-count":40,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,3]]}},"alternative-id":["10.1128\/JB.00841-06"],"URL":"https:\/\/doi.org\/10.1128\/jb.00841-06","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"type":"print","value":"0021-9193"},{"type":"electronic","value":"1098-5530"}],"subject":[],"published":{"date-parts":[[2007,3]]},"assertion":[{"value":"2006-06-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-12-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}