{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T17:11:20Z","timestamp":1764349880006},"reference-count":48,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>During spore formation in<jats:italic>Bacillus subtilis<\/jats:italic>, cell division occurs at the cell pole and is believed to require essentially the same division machinery as vegetative division. Intriguingly, although the cell division protein DivIB is not required for vegetative division at low temperatures, it is essential for efficient sporulation under these conditions. We show here that at low temperatures in the absence of DivIB, formation of the polar septum during sporulation is delayed and less efficient. Furthermore, the polar septa that are complete are abnormally thick, containing more peptidoglycan than a normal polar septum. These results show that DivIB is specifically required for the efficient and correct formation of a polar septum. This suggests that DivIB is required for the modification of sporulation septal peptidoglycan, raising the possibility that DivIB either regulates hydrolysis of polar septal peptidoglycan or is a hydrolase itself. We also show that, despite the significant number of completed polar septa that form in this mutant, it is unable to undergo engulfment. Instead, hydrolysis of the peptidoglycan within the polar septum, which occurs during the early stages of engulfment, is incomplete, producing a similar phenotype to that of mutants defective in the production of sporulation-specific septal peptidoglycan hydrolases. We propose a role for DivIB in sporulation-specific peptidoglycan remodelling or its regulation during polar septation and engulfment.<\/jats:p>","DOI":"10.1128\/jb.01072-06","type":"journal-article","created":{"date-parts":[[2006,10,18]],"date-time":"2006-10-18T23:23:35Z","timestamp":1161213815000},"page":"7677-7685","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Requirement for the Cell Division Protein DivIB in Polar Cell Division and Engulfment during Sporulation in<i>Bacillus subtilis<\/i>"],"prefix":"10.1128","volume":"188","author":[{"given":"L. S.","family":"Thompson","sequence":"first","affiliation":[{"name":"Institute for the Biotechnology of Infectious Diseases, University of Technology, Sydney, NSW 2007, Australia"}]},{"given":"P. L.","family":"Beech","sequence":"additional","affiliation":[{"name":"Centre for Cellular and Molecular Biology, School of Life and Environmental Sciences, Deakin University, Burwood, Vic 3125, Australia"}]},{"given":"G.","family":"Real","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Avenida da Repu\u0301blica, Apartado 127, 2781-901 Oeiras Codex, Portugal"}]},{"given":"A. O.","family":"Henriques","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, Avenida da Repu\u0301blica, Apartado 127, 2781-901 Oeiras Codex, Portugal"}]},{"given":"E. J.","family":"Harry","sequence":"additional","affiliation":[{"name":"Institute for the Biotechnology of Infectious Diseases, University of Technology, Sydney, NSW 2007, Australia"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.81.5.741-746.1961"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1111\/j.1365-2958.2005.04687.x","volume":"57","year":"2005","unstructured":"Barak, I., and A. J. Wilkinson. 2005. Where asymmetry in gene expression originates. Mol. Microbiol. 57 : 611-620.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.16.4984-4989.1996"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.12.6821-6834.1989"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"2196","DOI":"10.1016\/j.cub.2003.12.001","volume":"13","year":"2003","unstructured":"Ben-Yehuda, S., D. Z. Rudner, and R. Losick. 2003. Assembly of the SpoIIIE DNA translocase depends on chromosome trapping in Bacillus subtilis. Curr. Biol. 13 : 2196-2200.","journal-title":"Curr. Biol."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1079914"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.10.3511-3520.2005"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"14860","DOI":"10.1074\/jbc.M314274200","volume":"279","year":"2004","unstructured":"Carniol, K., P. Eichenberger, and R. Losick. 2004. A threshold mechanism governing activation of the developmental regulatory protein \u03c3F in Bacillus subtilis. J. Biol. Chem. 279 : 14860-14870.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_10_2","unstructured":"Cutting S. M. and P. B. Vander Home. 1990. Genetic analysis. John Wiley and Sons Chichester England."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1046\/j.1365-2958.2000.01857.x","volume":"36","year":"2000","unstructured":"Daniel, R. A., and J. Errington. 2000. Intrinsic instability of the essential cell division protein FtsL of Bacillus subtilis and a role for DivIB protein in FtsL turnover. Mol. Microbiol. 36 : 278-289.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1046\/j.1365-2958.2000.01724.x","volume":"35","year":"2000","unstructured":"Daniel, R. A., E. J. Harry, and J. Errington. 2000. Role of penicillin-binding protein PBP 2B in assembly and functioning of the division machinery of Bacillus subtilis. Mol. Microbiol. 35 : 299-311.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1046\/j.1365-2958.1998.00954.x","volume":"29","year":"1998","unstructured":"Daniel, R. A., E. J. Harry, V. L. Katis, R. G. Wake, and J. Errington. 1998. Characterization of the essential cell division gene ftsL (YllD) of Bacillus subtilis and its role in the assembly of the division apparatus. Mol. Microbiol. 29 : 593-604.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/nrmicro750","volume":"1","year":"2003","unstructured":"Errington, J. 2003. Regulation of endospore formation in Bacillus subtilis. Nat. Rev. Microbiol. 1 : 117-126.","journal-title":"Nat. Rev. Microbiol."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.67.1.52-65.2003"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/S0378-1119(01)00338-9","volume":"264","year":"2001","unstructured":"Feucht, A., and P. J. Lewis. 2001. Improved plasmid vectors for the production of multiple fluorescent protein fusions in Bacillus subtilis. Gene 264 : 289-297.","journal-title":"Gene"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1046\/j.1365-2958.2002.03082.x","volume":"45","year":"2002","unstructured":"Feucht, A., L. Abbotts, and J. Errington. 2002. The cell differentiation protein SpoIIE contains a regulatory site that controls its phosphatase activity in response to asymmetric septation. Mol. Microbiol. 45 : 1119-1130.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1046\/j.1365-2958.1999.01543.x","volume":"33","year":"1999","unstructured":"Feucht, A., R. A. Daniel, and J. Errington. 1999. Characterization of a morphological checkpoint coupling cell-specific transcription to septation in Bacillus subtilis. Mol. Microbiol. 33 : 1015-1026.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.3.716-722.1995"},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1101\/gad.11.9.1160","volume":"11","year":"1997","unstructured":"Glaser, P., M. Sharpe, B. Raether, M. Perego, K. Ohlsen, and J. Errington. 1997. Dynamic, mitotic-like behaviour of a bacterial protein required for accurate chromosome partitioning. Genes Dev. 11 : 1160-1168.","journal-title":"Genes Dev."},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"R514","DOI":"10.1016\/j.cub.2005.06.038","volume":"15","year":"2005","unstructured":"Goehring, N. W., and J. Beckwith. 2005. Diverse paths to midcell: assembly of the bacterial cell division machinery. Curr. Biol. 15 : R514-R526.","journal-title":"Curr. Biol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.13.4052-4060.2001"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1111\/j.1365-2958.1993.tb01152.x","volume":"7","year":"1993","unstructured":"Harry, E. J., B. J. Stewart, and R. G. Wake. 1993. Characterization of mutations in divIB of Bacillus subtilis and cellular localization of the DivIB protein. Mol. Microbiol. 7 : 611-621.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1046\/j.1365-2958.1997.4581822.x","volume":"25","year":"1997","unstructured":"Harry, E. J., and R. G. Wake. 1997. The membrane-bound cell division protein DivIB is localized to the division site in Bacillus subtilis. Mol. Microbiol. 25 : 275-283.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.68.2.234-262.2004"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.10.3159-3169.1991"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.9.2710-2718.1999"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.12.3607-3611.2000"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.14.3856-3863.2002"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.5.1256-1260.1998"},{"key":"e_1_3_2_31_2","first-page":"341","volume":"132","year":"1986","unstructured":"Lopez-Diaz, I., S. Clarke, and J. Mandelstam. 1986. SpoIID operon of Bacillus subtilis: cloning and sequence. J. Gen. Microbiol. 132 : 341-354.","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1038\/34472","volume":"391","year":"1998","unstructured":"Lowe, J., and L. A. Amos. 1998. Crystal structure of the bacterial cell-division protein FtsZ. Nature 391 : 203-206.","journal-title":"Nature"},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1126\/science.1948031","volume":"254","year":"1991","unstructured":"Margolis, P., A. Driks, and R. Losick. 1991. Establishment of cell type by compartmentalized activation of a transcription factor. Science 254 : 562-565.","journal-title":"Science"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.4.1096-1108.2000"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/br.40.4.908-962.1976"},{"key":"e_1_3_2_36_2","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.mib.2004.10.001","volume":"7","year":"2004","unstructured":"Piggot, P. J., and D. W. Hilbert. 2004. Sporulation of Bacillus subtilis. Curr. Opin. Microbiol. 7 : 579-586.","journal-title":"Curr. Opin. Microbiol."},{"key":"e_1_3_2_37_2","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1111\/j.1365-2958.2004.04399.x","volume":"55","year":"2005","unstructured":"Real, G., S. Autret, E. J. Harry, J. Errington, and A. O. Henriques. 2005. Cell division protein DivIB influences the SpoOJ\/Soj system of chromosome segregation in Bacillus subtilis. Mol. Microbiol. 55 : 349-367.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"6700","DOI":"10.1073\/pnas.0601397103","volume":"103","year":"2006","unstructured":"Robson, S. A., and G. F. King. 2006. Domain architecture and structure of the bacterial cell division protein DivIB. Proc. Natl. Acad. Sci. USA 103 : 6700-6705.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1046\/j.1365-2958.2002.02920.x","volume":"44","year":"2002","unstructured":"Robson, S. A., K. A. Michie, J. P. Mackay, E. J. Harry, M. D., and G. F. King. 2002. The Bacillus subtilis cell division proteins FtsL and DivIC are intrinsically unstable and do not interact with one another in the absence of other septasomal components. Mol. Microbiol. 44 : 663-674.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_40_2","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1046\/j.1365-2958.1997.2141580.x","volume":"23","year":"1997","unstructured":"Rowland, S. L., V. L. Katis, S. R. Partridge, and R. G. Wake. 1997. DivIB, FtsZ and cell division in Bacillus subtilis. Mol. Microbiol. 23 : 295-302.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.tibs.2003.08.003","volume":"28","year":"2003","unstructured":"Sanchez-Pulido, L., D. Devos, S. Genevrois, M. Vicente, and A. Valencia. 2003. POTRA: a conserved domain in the FtsQ family and a class of \u03b2-barrel outer membrane proteins. Trends Biochem. Sci. 28 : 523-526.","journal-title":"Trends Biochem. Sci."},{"key":"e_1_3_2_42_2","doi-asserted-by":"crossref","first-page":"14553","DOI":"10.1073\/pnas.96.25.14553","volume":"96","year":"1999","unstructured":"Sharp, M. D., and K. Pogliano. 1999. An in vivo membrane fusion assay implicates SpoIIIE in the final stages of engulfment during Bacillus subtilis sporulation. Proc. Natl. Acad. Sci. USA. 96 : 14553-14558.","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"e_1_3_2_43_2","doi-asserted-by":"crossref","first-page":"6267","DOI":"10.1093\/emboj\/cdf597","volume":"21","year":"2002","unstructured":"Sharp, M. D., and K. Pogliano. 2002. MinCD-dependent regulation of the polarity of SpoIIIE assembly and DNA transfer. EMBO J. 21 : 6267-6274.","journal-title":"EMBO J."},{"key":"e_1_3_2_44_2","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1046\/j.1365-2958.2000.01895.x","volume":"36","year":"2000","unstructured":"Sievers, J., and J. Errington. 2000. The Bacillus subtilis cell division protein FtsL localizes to sites of septation and interacts with DivIC. Mol. Microbiol. 36 : 846-855.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.11.3607-3617.1993"},{"key":"e_1_3_2_46_2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/0378-1119(94)90358-1","volume":"142","year":"1994","unstructured":"Steinmetz, M., and R. Richter. 1994. Plasmids designed to alter the antibiotic resistance expression by insertion mutations in Bacillus subtilis through in vivo recombination. Gene 142 : 79-83.","journal-title":"Gene"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.95.4.1439-1449.1968"},{"key":"e_1_3_2_48_2","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1126\/science.8160014","volume":"264","year":"1994","unstructured":"Wu, L. J., and J. Errington. 1994. Bacillus subtilis SpoIIIE protein required for DNA segregation during asymmetric cell division. Science 264 : 572-575.","journal-title":"Science"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03643.x"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.01072-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.01072-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T18:29:48Z","timestamp":1707330588000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.01072-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11]]},"references-count":48,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1128\/JB.01072-06"],"URL":"https:\/\/doi.org\/10.1128\/jb.01072-06","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11]]},"assertion":[{"value":"2006-07-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-08-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}