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In spite of its potential pathogenicity, this microorganism has several metabolic potentials that could be used in biotechnology applications.<jats:italic>K. pneumoniae<\/jats:italic>is able to metabolize glycerol as a sole source of carbon and energy. 1,3-Propanediol dehydrogenase is the core of the metabolic pathway for the use of glycerol. We have determined the crystallographic structure of 1,3-propanediol dehydrogenase, a type III Fe-NAD-dependent alcohol dehydrogenase, at 2.7-A\u030a resolution. The structure of the enzyme monomer is closely related to that of other alcohol dehydrogenases. The overall arrangement of the enzyme showed a decameric structure, formed by a pentamer of dimers, which is the catalytic form of the enzyme. Dimers are associated by strong ionic interactions that are responsible for the highly stable in vivo packing of the enzyme. Kinetic properties of the enzyme as determined in the article would suggest that this decameric arrangement is related to the cooperativity between monomers.<\/jats:p>","DOI":"10.1128\/jb.01077-08","type":"journal-article","created":{"date-parts":[[2008,11,15]],"date-time":"2008-11-15T01:44:43Z","timestamp":1226713483000},"page":"1143-1151","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["1,3-Propanediol Dehydrogenase from<i>Klebsiella pneumoniae<\/i>: Decameric Quaternary Structure and Possible Subunit Cooperativity"],"prefix":"10.1128","volume":"191","author":[{"given":"David","family":"Marc\u0327al","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, 2781-901 Oeiras, Portugal"}]},{"given":"Ana Toste","family":"Re\u0302go","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, 2781-901 Oeiras, Portugal"}]},{"given":"Maria Arme\u0301nia","family":"Carrondo","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, 2781-901 Oeiras, Portugal"}]},{"given":"Francisco J.","family":"Enguita","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica, Universidade Nova de Lisboa, 2781-901 Oeiras, Portugal"},{"name":"Unidade de Biologia Celular, Instituto de Medicina Molecular, Universidade de Lisboa, 1649-028 Lisbon, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1002\/(SICI)1097-0290(19980905)59:5<544::AID-BIT3>3.0.CO;2-A","volume":"59","year":"1998","unstructured":"Ahrens, K., K. 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