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Among them, the type II polyketide synthase-encoding<jats:italic>alp<\/jats:italic>cluster was shown to be responsible for the biosynthesis of a compound with antibacterial activity. Here, by means of a deregulation approach, we gained access to workable amounts of the antibiotics for structure elucidation. These compounds, previously designated as alpomycin, were shown to be known members of kinamycin family of antibiotics. Indeed, a mutant lacking AlpW, a member of the TetR regulator family, was shown to constitutively produce kinamycins. Comparative transcriptional analyses showed that expression of<jats:italic>alpV<\/jats:italic>, the essential regulator gene required for activation of the biosynthetic genes, is strongly maintained during the stationary growth phase in the<jats:italic>alpW<\/jats:italic>mutant, a stage at which<jats:italic>alpV<\/jats:italic>transcripts and thereby transcripts of the biosynthetic genes normally drop off. Recombinant AlpW displayed DNA binding activity toward specific motifs in the promoter region of its own gene and that of<jats:italic>alpV<\/jats:italic>and<jats:italic>alpZ<\/jats:italic>. These recognition sequences are also targets for AlpZ, the \u03b3-butyrolactone-like receptor involved in the regulation of the<jats:italic>alp<\/jats:italic>cluster. However, unlike that of AlpZ, the AlpW DNA-binding ability seemed to be insensitive to the signaling molecules controlling antibiotic biosynthesis. Together, the results presented in this study reveal<jats:italic>S. ambofaciens<\/jats:italic>to be a new producer of kinamycins and AlpW to be a key late repressor of the cellular control of kinamycin biosynthesis.<\/jats:p>","DOI":"10.1128\/jb.01269-10","type":"journal-article","created":{"date-parts":[[2010,12,31]],"date-time":"2010-12-31T03:43:31Z","timestamp":1293767011000},"page":"1142-1153","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":93,"title":["Characterization and Manipulation of the Pathway-Specific Late Regulator AlpW Reveals<i>Streptomyces ambofaciens<\/i>as a New Producer of Kinamycins"],"prefix":"10.1128","volume":"193","author":[{"given":"Robert","family":"Bunet","sequence":"first","affiliation":[{"name":"Universite\u0301 Henri Poincare\u0301, UMR1128 Ge\u0301ne\u0301tique et Microbiologie, IFR110 EFABA, F-54506 Vand\u0153uvre-le\u0300s-Nancy, France"},{"name":"INRA, UMR1128 Ge\u0301ne\u0301tique et Microbiologie, F-54506 Vand\u0153uvre-le\u0300s-Nancy, France"}]},{"given":"Lijiang","family":"Song","sequence":"additional","affiliation":[{"name":"University of Warwick, Department of Chemistry, CV4 7AL Coventry, United Kingdom"}]},{"given":"Marta Vaz","family":"Mendes","sequence":"additional","affiliation":[{"name":"Universite\u0301 Henri Poincare\u0301, UMR1128 Ge\u0301ne\u0301tique et Microbiologie, IFR110 EFABA, F-54506 Vand\u0153uvre-le\u0300s-Nancy, France"},{"name":"INRA, UMR1128 Ge\u0301ne\u0301tique et Microbiologie, F-54506 Vand\u0153uvre-le\u0300s-Nancy, France"},{"name":"Present address: IBMC\u2014Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre, 823 Oporto, Portugal."}]},{"given":"Christophe","family":"Corre","sequence":"additional","affiliation":[{"name":"University of Warwick, Department of Chemistry, CV4 7AL Coventry, United Kingdom"}]},{"given":"Laurence","family":"Hotel","sequence":"additional","affiliation":[{"name":"Universite\u0301 Henri Poincare\u0301, UMR1128 Ge\u0301ne\u0301tique et Microbiologie, IFR110 EFABA, F-54506 Vand\u0153uvre-le\u0300s-Nancy, France"},{"name":"INRA, UMR1128 Ge\u0301ne\u0301tique et Microbiologie, F-54506 Vand\u0153uvre-le\u0300s-Nancy, France"}]},{"given":"Nicolas","family":"Rouhier","sequence":"additional","affiliation":[{"name":"Universite\u0301 Henri Poincare\u0301, UMR1136 Interactions Arbres\/Micro-organismes, IFR110 EFABA, F-54506 Vand\u0153uvre-le\u0300s-Nancy, France"},{"name":"INRA, UMR1136 Interactions Arbres\/Micro-organismes, F-54506 Vand\u0153uvre-le\u0300s-Nancy, France"}]},{"given":"Xavier","family":"Framboisier","sequence":"additional","affiliation":[{"name":"CNRS, UPR3349 Laboratoire Re\u0301actions et Ge\u0301nie des Proce\u0301de\u0301s, F-54001 Nancy, France"}]},{"given":"Pierre","family":"Leblond","sequence":"additional","affiliation":[{"name":"Universite\u0301 Henri Poincare\u0301, UMR1128 Ge\u0301ne\u0301tique et Microbiologie, IFR110 EFABA, F-54506 Vand\u0153uvre-le\u0300s-Nancy, France"},{"name":"INRA, UMR1128 Ge\u0301ne\u0301tique et Microbiologie, F-54506 Vand\u0153uvre-le\u0300s-Nancy, France"}]},{"given":"Gregory L.","family":"Challis","sequence":"additional","affiliation":[{"name":"University of Warwick, Department of Chemistry, CV4 7AL Coventry, United Kingdom"}]},{"given":"Bertrand","family":"Aigle","sequence":"additional","affiliation":[{"name":"Universite\u0301 Henri Poincare\u0301, UMR1128 Ge\u0301ne\u0301tique et Microbiologie, IFR110 EFABA, F-54506 Vand\u0153uvre-le\u0300s-Nancy, France"},{"name":"INRA, UMR1128 Ge\u0301ne\u0301tique et Microbiologie, F-54506 Vand\u0153uvre-le\u0300s-Nancy, France"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.7.2491-2500.2005"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2006\/002170-0"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.29161-0"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(99)80113-6"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/417141a"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(92)90627-2"},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1111\/j.1365-2958.2006.05541.x","volume":"63","year":"2007","unstructured":"Bignell, D. 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