{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T21:38:42Z","timestamp":1772919522505,"version":"3.50.1"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T00:00:00Z","timestamp":1201824000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In the unicellular cyanobacterium\n            <jats:italic>Synechocystis<\/jats:italic>\n            sp. strain PCC 6803, the pentameric bidirectional Ni-Fe hydrogenase (HoxEFUYH) is the sole enzyme involved in hydrogen metabolism. Recent investigations implicated the transcription factor LexA in the regulation of the\n            <jats:italic>hox<\/jats:italic>\n            genes in this cyanobacterium, suggesting the factor to work as an activator. In this work, we show evidence that LexA cannot account exclusively for the regulation of the\n            <jats:italic>hox<\/jats:italic>\n            genes in this cyanobacterium. Therefore, we investigated which additional transcription factors interact in and may regulate the expression of the\n            <jats:italic>hox<\/jats:italic>\n            genes in\n            <jats:italic>Synechocystis<\/jats:italic>\n            sp. strain PCC 6803. By using DNA affinity assays, a transcription factor with similarity to the transition state regulator AbrB from\n            <jats:italic>Bacillus subtilis<\/jats:italic>\n            was isolated. Electrophoretic mobility shift assays showed that the AbrB-like protein specifically interacts with the promoter region of the\n            <jats:italic>hox<\/jats:italic>\n            genes as well as with its own promoter region. In addition, results obtained with two genetically modified strains of\n            <jats:italic>Synechocystis<\/jats:italic>\n            sp. strain PCC 6803, one with a not fully segregated inactivation mutation of the\n            <jats:italic>abrB<\/jats:italic>\n            -like gene and the other overexpressing the same\n            <jats:italic>abrB<\/jats:italic>\n            -like gene, suggest that this transcription factor functions as a regulator of\n            <jats:italic>hox<\/jats:italic>\n            gene expression.\n          <\/jats:p>","DOI":"10.1128\/jb.01605-07","type":"journal-article","created":{"date-parts":[[2007,11,27]],"date-time":"2007-11-27T04:40:31Z","timestamp":1196138431000},"page":"1011-1019","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["An AbrB-Like Protein Regulates the Expression of the Bidirectional Hydrogenase in\n            <i>Synechocystis<\/i>\n            sp. Strain PCC 6803"],"prefix":"10.1128","volume":"190","author":[{"given":"Paulo","family":"Oliveira","sequence":"first","affiliation":[{"name":"Department of Photochemistry and Molecular Science, A\u030angstro\u0308m Laboratories, Uppsala University, P.O. Box 523, SE-751 20 Uppsala, Sweden"}]},{"given":"Peter","family":"Lindblad","sequence":"additional","affiliation":[{"name":"Department of Photochemistry and Molecular Science, A\u030angstro\u0308m Laboratories, Uppsala University, P.O. Box 523, SE-751 20 Uppsala, Sweden"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1016\/j.ijhydene.2006.06.037","volume":"31","year":"2006","unstructured":"Antal, T. K., P. Oliveira, and P. Lindblad. 2006. The bidirectional hydrogenase in the cyanobacterium Synechocystis sp. strain PCC 6803. Int. J. Hydrogen Energy 31 : 1439-1444.","journal-title":"Int. J. Hydrogen Energy"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.1.444-447.2002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1111\/j.1574-6968.1999.tb13357.x","volume":"170","year":"1999","unstructured":"Axelsson, R., F. Oxelfelt, and P. Lindblad. 1999. Transcriptional regulation of Nostoc uptake hydrogenase. FEMS Microbiol. Lett. 170 : 77-81.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"4034","DOI":"10.1093\/emboj\/16.13.4034","volume":"16","year":"1997","unstructured":"Barne, K. A., J. A. Bown, S. J. W. Busby, and S. D. Minchin. 1997. Region 2.5 of the Escherichia coli RNA polymerase sigma70 subunit is responsible for the recognition of the \u2018extended-10\u2019 motif at promoters. EMBO J. 16 : 4034-4040.","journal-title":"EMBO J."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"16106","DOI":"10.1021\/bi048399h","volume":"43","year":"2004","unstructured":"Bobay, B. G., L. Benson, S. Naylor, B. Feeney, A. C. Clark, M. B. Goshe, M. A. Strauch, R. Thompson, and J. Cavanagh. 2004. Evaluation of the DNA binding tendencies of the transition state regulator AbrB. Biochemistry 43 : 16106-16118.","journal-title":"Biochemistry"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"21399","DOI":"10.1074\/jbc.M601963200","volume":"281","year":"2006","unstructured":"Bobay, B. G., G. A. Mueller, R. J. Thompson, A. G. Murzin, R. A. Venters, M. A. Strauch, and J. Cavanagh. 2006. NMR structure of AbhN and comparison with AbrBN: first insights into the DNA binding promiscuity and specificity of AbrB-like transition state regulator proteins. J. Biol. Chem. 281 : 21399-21409.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1146\/annurev.pp.26.060175.002101","volume":"26","year":"1975","unstructured":"Bogorad, L. 1975. Phycobiliproteins and complementary chromatic adaptation. Annu. Rev. Plant Physiol. 26 : 369-401.","journal-title":"Annu. Rev. Plant Physiol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1007\/s00216-004-3020-4","volume":"382","year":"2005","unstructured":"Colyer, C. L., C. S. Kinkade, P. J. Viskari, and J. P. Landers. 2005. Analysis of cyanobacterial pigments and proteins by electrophoretic and chromatographic methods. Anal. Bioanal. Chem. 382 : 559-569.","journal-title":"Anal. Bioanal. Chem."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.12.3019-3025.1998"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/j.1365-2958.2004.04100.x","volume":"53","year":"2004","unstructured":"Domain, F., L. Houot, F. Chauvat, and C. Cassier-Chauvat. 2004. Function and regulation of the cyanobacterial genes lexA, recA and ruvB: LexA is critical to the survival of cells facing inorganic carbon starvation. Mol. Microbiol. 53 : 65-80.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_13_2","unstructured":"Expression and regulation of membrane proteins: special focus on cytochrome bd-oxidase from Synechocystis sp. PCC 6803. 2006"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/0076-6879(88)67086-8","volume":"167","year":"1988","unstructured":"Elhai, J., and C. P. Wolk. 1988. Conjugal transfer of DNA to cyanobacteria, p. 747-754. In L. Packer and A. N. Glazer (ed.), Methods in enzymology, vol. 167. Academic Press, Inc., San Diego, CA.","journal-title":"Methods in enzymology"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1111\/j.1365-2958.2006.05231.x","volume":"61","year":"2006","unstructured":"Espinosa, J., K. Forchhammer, S. Burillo, and A. Contreras. 2006. Interaction network in cyanobacterial nitrogen regulation: PipX, a protein that interacts in a 2-oxoglutarate dependent manner with PII and NtcA. Mol. Microbiol. 61 : 457-469.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1007\/s00203-007-0281-2","volume":"188","year":"2007","unstructured":"Ferreira, D., E. Leit\u00e3o, J. Sj\u00f6holm, P. Oliveira, P. Lindblad, P. Moradas-Ferreira, and P. Tamagnini. 2007. Transcription and regulation of the hydrogenase(s) accessory genes, hypFCDEAB, in the cyanobacterium Lyngbya majuscula CCAP 1446\/4. Arch. Microbiol. 188 : 609-617.","journal-title":"Arch. Microbiol."},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"2468","DOI":"10.1002\/pmic.200401266","volume":"5","year":"2005","unstructured":"Gan, C. S., K. F. Reardon, and P. C. Wright. 2005. Comparison of protein and peptide prefractionation methods for the shotgun proteomic analysis of Synechocystis sp. PCC 6803. Proteomics 5 : 2468-2478.","journal-title":"Proteomics"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1111\/j.1365-2958.2005.04867.x","volume":"58","year":"2005","unstructured":"Gutekunst, K., S. Phunpruch, C. Schwarz, S. Schuchardt, R. Schulz-Friedrich, and J. Appel. 2005. LexA regulates the bidirectional hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803 as a transcription activator. Mol. Microbiol. 58 : 810-823.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_19_2","unstructured":"Heterologe \u00dcberexpression in cyanobakterien. 2001"},{"key":"e_1_3_2_20_2","first-page":"S87","volume":"47","year":"2006","unstructured":"Ishii, A., and Y. Hihara. 2006. Characterization of putative transcriptional regulators having an AbrB-type DNA binding domain in Synechocystis sp. PCC 6803. Plant Cell Physiol. 47 : S87.","journal-title":"Plant Cell Physiol."},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1093\/dnares\/3.3.185","volume":"3","year":"1996","unstructured":"Kaneko, T., S. Sato, H. Kotani, A. Tanaka, E. Asamizu, Y. Nakamura, N. Miyajima, M. Hirosawa, M. Sugiura, S. Sasamoto, T. Kimura, T. Hosouchi, A. Matsuno, A. Muraki, N. Nakazaki, K. Naruo, S. Okumura, S. Shimpo, C. Takeuchi, T. Wada, A. Watanabe, M. Yamada, M. Yasuda, and S. Tabata. 1996. Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions (supplement). DNA Res. 3 : 185-209.","journal-title":"DNA Res."},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/j.1574-6968.2002.tb11012.x","volume":"206","year":"2002","unstructured":"Kufryk, G. I., M. Sachet, G. Schmetterer, and W. F. J. Vermaas. 2002. Transformation of the cyanobacterium Synechocystis sp. PCC 6803 as a tool for genetic mapping: optimization of efficiency. FEMS Microbiol. Lett. 206 : 215-219.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.6.3449-3457.1989"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.8.4567-4576.2005"},{"key":"e_1_3_2_25_2","unstructured":"Cyanobacterial hydrogen metabolism\u2014uptake hydrogenase and hydrogen production by nitrogenase in filamentous cyanobacteria. 2003"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0014-5793(99)00074-5","volume":"444","year":"1999","unstructured":"Malakhov, M. P., O. A. Malakhova, and N. Murata. 1999. Balanced regulation of expression of the gene for cytochrome cM and that of genes for plastocyanin and cytochrome c6 in Synechocystis. FEBS Lett. 444 : 281-284.","journal-title":"FEBS Lett."},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"31463","DOI":"10.1074\/jbc.273.47.31463","volume":"273","year":"1998","unstructured":"Montesinos, M. L., A. M. Muro-Pastor, A. Herrero, and E. Flores. 1998. Ammonium\/methylammonium permeases of a cyanobacterium: identification and analysis of three nitrogen-regulated amt genes in Synechocystis sp. PCC 6803. J. Biol. Chem. 273 : 31463-31470.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"3647","DOI":"10.1099\/mic.0.27248-0","volume":"150","year":"2004","unstructured":"Oliveira, P., E. Leit\u00e3o, P. Tamagnini, P. Moradas-Ferreira, and F. Oxelfelt. 2004. Characterization and transcriptional analysis of hupSLW in Gloeothece sp. ATCC 27152: an uptake hydrogenase from a unicellular cyanobacterium. Microbiology 150 : 3647-3655.","journal-title":"Microbiology"},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.femsle.2005.07.024","volume":"251","year":"2005","unstructured":"Oliveira, P., and P. Lindblad. 2005. LexA, a transcription regulator binding in the promoter region of the bidirectional hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803. FEMS Microbiol. Lett. 251 : 59-66.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_30_2","first-page":"149","year":"2000","unstructured":"Oliver, R. L., and G. G. Ganf. 2000. Freshwater blooms, p. 149-194. In B. A. Whitton and M. Potts (ed.), The ecology of cyanobacteria. Kluwer Academic Publishers, Dordrecht, The Netherlands.","journal-title":"The ecology of cyanobacteria"},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"3446","DOI":"10.1093\/nar\/gkl426","volume":"34","year":"2006","unstructured":"Patterson-Fortin, L. M., K. R. Colvin, and G. W. Owttrim. 2006. A LexA-related protein regulates redox-sensitive expression of the cyanobacterial RNA helicase, crhR. Nucleic Acids Res. 34 : 3446-3454.","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"2160","DOI":"10.1002\/(SICI)1522-2683(19990801)20:11<2160::AID-ELPS2160>3.0.CO;2-#","volume":"20","year":"1999","unstructured":"Sazuka, T., M. Yamaguchi, and O. Ohara. 1999. Cyano2Dbase updated: linkage of 234 protein spots to corresponding genes through N-terminal microsequencing. Electrophoresis 20 : 2160-2171.","journal-title":"Electrophoresis"},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1007\/s00425-003-1113-5","volume":"218","year":"2004","unstructured":"Sch\u00fctz, K., T. Happe, O. Troshina, P. Lindblad, E. Leit\u00e3o, P. Oliveira, and P. Tamagnini. 2004. Cyanobacterial H2 production\u2014a comparative analysis. Planta 218 : 350-359.","journal-title":"Planta"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00756-07"},{"key":"e_1_3_2_35_2","doi-asserted-by":"crossref","first-page":"4905","DOI":"10.1002\/pmic.200500111","volume":"5","year":"2005","unstructured":"Srivastava, R., T. Pisareva, and B. Norling. 2005. Proteomic studies of the thylakoid membrane of Synechocystis sp. PCC 6803. Proteomics 5 : 4905-4916.","journal-title":"Proteomics"},{"key":"e_1_3_2_36_2","first-page":"171","volume":"35","year":"1971","unstructured":"Stanier, R. Y., R. Kunisawa, M. Mandel, and G. Cohen-Bazire. 1971. Purification and properties of unicellular blue-green algae (order Chroococcales). Microbiol. Mol. Biol. Rev. 35 : 171-205.","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"e_1_3_2_37_2","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1104\/pp.104.059097","volume":"138","year":"2005","unstructured":"Suzuki, I., Y. Kanesaki, H. Hayashi, J. J. Hall, W. J. Simon, A. R. Slabas, and N. Murata. 2005. The histidine kinase Hik34 is involved in thermotolerance by regulating the expression of heat shock genes in Synechocystis. Plant Physiol. 138 : 1409-1421.","journal-title":"Plant Physiol."},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.66.1.1-20.2002"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1111\/j.1574-6976.2007.00085.x","volume":"31","year":"2007","unstructured":"Tamagnini, P., E. Leit\u00e3o, P. Oliveira, D. Ferreira, F. Pinto, D. J. Harris, T. Heidorn, and P. Lindblad. 2007. Cyanobacterial hydrogenases: diversity, regulation and applications. FEMS Microbiol. Rev. 31 : 692-720.","journal-title":"FEMS Microbiol. Rev."},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.5.1801-1807.1997"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.21.7337-7343.2004"},{"key":"e_1_3_2_43_2","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1038\/81999","volume":"7","year":"2000","unstructured":"Vaughn, J. L., V. Feher, S. Naylor, M. A. Strauch, and J. Cavanagh. 2000. Novel DNA binding domain and genetic regulation model of Bacillus subtilis transition state regulator AbrB. Nat. Struct. Mol. Biol. 7 : 1139-1146.","journal-title":"Nat. Struct. Mol. Biol."},{"key":"e_1_3_2_44_2","doi-asserted-by":"crossref","first-page":"6331","DOI":"10.1093\/nar\/20.23.6331","volume":"20","year":"1992","unstructured":"Vioque, A. 1992. Analysis of the gene encoding the RNA subunit of ribonuclease P from cyanobacteria. Nucleic Acids Res. 20 : 6331-6337.","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_45_2","doi-asserted-by":"crossref","first-page":"2327","DOI":"10.1002\/1522-2683(20017)22:11<2327::AID-ELPS2327>3.0.CO;2-J","volume":"22","year":"2001","unstructured":"Viskari, P. J., C. S. Kinkade, and C. L. Colyer. 2001. Determination of phycobiliproteins by capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis 22 : 2327-2335.","journal-title":"Electrophoresis"},{"key":"e_1_3_2_46_2","doi-asserted-by":"crossref","first-page":"1746","DOI":"10.1002\/(SICI)1522-2683(20000501)21:9<1746::AID-ELPS1746>3.0.CO;2-O","volume":"21","year":"2000","unstructured":"Wang, Y., J. Sun, and P. R. Chitnis. 2000. Proteomic study of the peripheral proteins from thylakoid membranes of the cyanobacterium Synechocystis sp. PCC 6803. Electrophoresis 21 : 1746-1754.","journal-title":"Electrophoresis"},{"key":"e_1_3_2_47_2","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1083\/jcb.85.3.558","volume":"85","year":"1980","unstructured":"Williams, R. C., J. C. Gingrich, and A. N. Glazer. 1980. Cyanobacterial phycobilisomes. Particles from Synechocystis 6701 and two pigment mutants. J. Cell Biol. 85 : 558-566.","journal-title":"J. Cell Biol."},{"key":"e_1_3_2_48_2","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1038\/379466a0","volume":"379","year":"1996","unstructured":"Wilm, M., A. Shevchenko, T. Houthaeve, S. Breit, L. Schweigerer, T. Fotsis, and M. Mann. 1996. Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry. Nature 379 : 466-469.","journal-title":"Nature"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.13.4094-4098.2001"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.01605-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.01605-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:43:41Z","timestamp":1627580621000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.01605-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,2]]},"references-count":48,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2008,2]]}},"alternative-id":["10.1128\/JB.01605-07"],"URL":"https:\/\/doi.org\/10.1128\/jb.01605-07","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,2]]},"assertion":[{"value":"2007-10-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-11-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}