{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T15:58:12Z","timestamp":1761580692979},"reference-count":16,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2009,8,1]],"date-time":"2009-08-01T00:00:00Z","timestamp":1249084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The objectives of this study were to evaluate the reproducibility of a molecular method for the subtyping of\n            <jats:italic>Treponema pallidum<\/jats:italic>\n            subsp.\n            <jats:italic>pallidum<\/jats:italic>\n            and to discriminate strains of this microorganism from strains from patients with syphilis. We studied 212 specimens from a total of 82 patients with different stages of syphilis (14 primary, 7 secondary and 61 latent syphilis). The specimens were distributed as follows: genital ulcers (\n            <jats:italic>n<\/jats:italic>\n            = 9), skin and mucosal lesions (\n            <jats:italic>n<\/jats:italic>\n            = 7), blood (\n            <jats:italic>n<\/jats:italic>\n            = 82), plasma (\n            <jats:italic>n<\/jats:italic>\n            = 82), and ear lobe scrapings (\n            <jats:italic>n<\/jats:italic>\n            = 32). The samples were assayed by a PCR technique to amplify a segment of the polymerase gene I (\n            <jats:italic>polA<\/jats:italic>\n            ). Positive samples were typed on the basis of the analysis of two variable genes,\n            <jats:italic>tpr<\/jats:italic>\n            and\n            <jats:italic>arp<\/jats:italic>\n            . Sixty-two of the 90 samples positive for\n            <jats:italic>polA<\/jats:italic>\n            yielded typeable\n            <jats:italic>Treponema pallidum<\/jats:italic>\n            DNA. All skin lesions in which\n            <jats:italic>T. pallidum<\/jats:italic>\n            was identified (six of six [100%]) were found to contain enough DNA for typing of the organism. It was also possible to type DNA from 7\/9 (77.7%) genital ulcer samples, 13\/22 (59.1%) blood samples, 20\/32 (62.5%) plasma samples, and 16\/21 (76.2%) ear lobe scrapings. The same subtype was identified in all samples from the same patient. Five molecular subtypes (subtypes 10a, 14a, 14c, 14f, and 14g) were identified, with the most frequently found subtype being subtype 14a and the least frequently found subtype being subtype 10a. In conclusion, the subtyping technique used in this study seems to have good reproducibility. To our knowledge, subtype 10a was identified for the first time. Further studies are needed to explain the presence of this subtype in Portugal, namely, its relationship to the\n            <jats:italic>Treponema pallidum<\/jats:italic>\n            strains circulating in the African countries where Portuguese is spoken.\n          <\/jats:p>","DOI":"10.1128\/jcm.00287-08","type":"journal-article","created":{"date-parts":[[2009,6,4]],"date-time":"2009-06-04T01:35:55Z","timestamp":1244079355000},"page":"2510-2512","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Molecular Subtyping of\n            <i>Treponema pallidum<\/i>\n            subsp.\n            <i>pallidum<\/i>\n            in Lisbon, Portugal"],"prefix":"10.1128","volume":"47","author":[{"given":"R.","family":"Castro","sequence":"first","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical, Unidade de Doenc\u0327as Sexualmente Transmitidas, Universidade Nova de Lisboa"}]},{"given":"E.","family":"Prieto","sequence":"additional","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical, Unidade de Doenc\u0327as Sexualmente Transmitidas, Universidade Nova de Lisboa"}]},{"given":"M. J.","family":"A\u0301guas","sequence":"additional","affiliation":[{"name":"Hospital Garcia de Orta, Lisbon, Portugal"}]},{"given":"M. J.","family":"Manata","sequence":"additional","affiliation":[{"name":"Hospital Garcia de Orta, Lisbon, Portugal"}]},{"given":"J.","family":"Botas","sequence":"additional","affiliation":[{"name":"Hospital Garcia de Orta, Lisbon, Portugal"}]},{"given":"F.","family":"Martins Pereira","sequence":"additional","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical, Unidade de Doenc\u0327as Sexualmente Transmitidas, Universidade Nova de Lisboa"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAI.0b013e31815e4011"},{"key":"e_1_3_1_3_2","first-page":"26","year":"2007","unstructured":"Direc\u00e7\u00e3o de Servi\u00e7os de Informa\u00e7\u00e3o e An\u00e1lise, Divis\u00e3o de Epidemiologia. 2007. Doen\u00e7as de declara\u00e7\u00e3o obrigat\u00f3ria 2002-2006. Regi\u00f5es e sub-regi\u00f5es de sa\u00fade no continente, regi\u00f5es aut\u00f3nomas, p. 26. Direc\u00e7\u00e3o de Servi\u00e7os de Informa\u00e7\u00e3o e An\u00e1lise, Divis\u00e3o de Epidemiologia, Direc\u00e7\u00e3o-Geral da Sa\u00fade, Lisbon, Portugal. http:\/\/www.dgs.pt\/ , Estatisticas link.","journal-title":"Doen\u00e7as de declara\u00e7\u00e3o obrigat\u00f3ria 2002-2006. Regi\u00f5es e sub-regi\u00f5es de sa\u00fade no continente, regi\u00f5es aut\u00f3nomas"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.75.1.3"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00128-08"},{"key":"e_1_3_1_6_2","unstructured":"Reference deleted."},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.annepidem.2004.05.001"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31817f97a0"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.5.1941-1946.2001"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46956-0"},{"key":"e_1_3_1_11_2","first-page":"189","volume":"83","year":"2007","unstructured":"Molepo, J., A. Pillay, B. Weber, S. A. Morse, and A. A. Hoosen. 2007. Molecular typing of Treponema pallidum strains from patients with neurosyphilis in Pretoria, South Africa. Sex. Transm. Infect.83:189-192.","journal-title":"Sex. Transm. Infect."},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.10.3077-3078.1998"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.1.256-258.2002"},{"key":"e_1_3_1_14_2","first-page":"408","volume":"8","year":"1998","unstructured":"Pillay, A., H. Liu, C. Y. Chen, B. Holloway, A. W. Sturm, B. Steiner, and S. A. Morse. 1998. Molecular subtyping of Treponema pallidum subspecies pallidum. Sex. Transm. Dis.8:408-414.","journal-title":"Sex. Transm. Dis."},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.8.3743-3746.2005"},{"key":"e_1_3_1_16_2","first-page":"461","volume":"5","year":"2008","unstructured":"Porras, C., M. Safaeian, P. Gonz\u00e1lez, A. Hildesheim, S. Silva, M. Schiffman, A. C. Rodr\u00edguez, S. Wacholder, E. Freer, K. Quint, C. Bratti, A. Espinoza, B. Cortes, R. Herrero, and Costa Rica HPV Vaccine Trial (CVT) Group. 2008. Epidemiology of genital Chlamydia trachomatis infection among young women in Costa Rica. Sex. Transm. Dis.5:461-468.","journal-title":"Sex. Transm. Dis."},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1086\/320698"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00287-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00287-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T07:03:11Z","timestamp":1646463791000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00287-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8]]},"references-count":16,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2009,8]]}},"alternative-id":["10.1128\/JCM.00287-08"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00287-08","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8]]},"assertion":[{"value":"2008-02-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-05-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}