{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T15:58:23Z","timestamp":1761580703048},"reference-count":35,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2009,9,1]],"date-time":"2009-09-01T00:00:00Z","timestamp":1251763200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A multilocus microsatellite typing (MLMT) approach based on the analysis of 15 independent loci has been developed for the discrimination of strains belonging to different\n            <jats:italic>Viannia<\/jats:italic>\n            species. Thirteen microsatellite loci were isolated de novo from microsatellite-enriched libraries for both\n            <jats:italic>Leishmania braziliensis<\/jats:italic>\n            and\n            <jats:italic>L. guyanensis<\/jats:italic>\n            . Two previously identified markers, AC01 and AC16, were modified and added to our marker set. Markers were designed to contain simple dinucleotide repeats flanked by the minimal possible number of nucleotides in order to allow variations in repeat numbers to be scored as size variations of the PCR products. The 15 markers in total were amplified for almost all of the strains of\n            <jats:italic>Viannia<\/jats:italic>\n            tested; one marker did not amplify from the two\n            <jats:italic>L. peruviana<\/jats:italic>\n            strains included in the study. When 30 strains of\n            <jats:italic>L. braziliensis<\/jats:italic>\n            , 21 strains of\n            <jats:italic>L. guyanensis<\/jats:italic>\n            , and 2 strains of\n            <jats:italic>L. peruviana<\/jats:italic>\n            were tested for polymorphisms, all strains except two strains of\n            <jats:italic>L. guyanensis<\/jats:italic>\n            had individual MLMT types. Distance-based analysis identified three main clusters. All strains except one strain of\n            <jats:italic>L. guyanensis<\/jats:italic>\n            grouped together. Two clusters consisted of strains of\n            <jats:italic>L. braziliensis<\/jats:italic>\n            according to their geographical origins. The two strains of\n            <jats:italic>L. peruviana<\/jats:italic>\n            grouped together with strains of\n            <jats:italic>L. braziliensis<\/jats:italic>\n            from Peru and the adjacent Brazilian state of Acre. MLMT has proven capable of individualizing strains even from the same areas of endemicity and of detecting genetic structures at different levels. MLMT is thus applicable for epidemiological and population genetic studies of strains within the subgenus\n            <jats:italic>Viannia<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.00645-09","type":"journal-article","created":{"date-parts":[[2009,7,8]],"date-time":"2009-07-08T22:06:57Z","timestamp":1247090817000},"page":"2818-2825","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Development of a Multilocus Microsatellite Typing Approach for Discriminating Strains of\n            <i>Leishmania<\/i>\n            (\n            <i>Viannia<\/i>\n            ) Species"],"prefix":"10.1128","volume":"47","author":[{"given":"Rolando","family":"Oddone","sequence":"first","affiliation":[{"name":"Institut fu\u0308r Mikrobiologie und Hygiene, Universita\u0308tsmedizin Berlin, Dorotheenstr. 96, Berlin 10117, Germany"},{"name":"Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asuncio\u0301n, Rio de la Plata y Lagerenza, Asuncio\u0301n, Paraguay"}]},{"given":"Carola","family":"Schweynoch","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Mikrobiologie und Hygiene, Universita\u0308tsmedizin Berlin, Dorotheenstr. 96, Berlin 10117, Germany"}]},{"given":"Gabriele","family":"Scho\u0308nian","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Mikrobiologie und Hygiene, Universita\u0308tsmedizin Berlin, Dorotheenstr. 96, Berlin 10117, Germany"}]},{"given":"Cintia","family":"dos Santos de Sousa","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365-Pav Leonidas Deane, Rio de Janeiro 21040-900, Brazil"}]},{"given":"Elisa","family":"Cupolillo","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365-Pav Leonidas Deane, Rio de Janeiro 21040-900, Brazil"}]},{"given":"Diego","family":"Espinosa","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical Alexander von Humboldt, Av. Honorio Delgado 430, San Martin de Porres, Lima 100, Peru"}]},{"given":"Jorge","family":"Arevalo","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical Alexander von Humboldt, Av. Honorio Delgado 430, San Martin de Porres, Lima 100, Peru"}]},{"given":"Harry","family":"Noyes","sequence":"additional","affiliation":[{"name":"Biosciences Department, University of Liverpool, Crown St., Liverpool L69 7ZB, United Kingdom"}]},{"given":"Isabel","family":"Mauricio","sequence":"additional","affiliation":[{"name":"London School of Hygiene and Tropical Medicine, Keppel Street, London WC1 E7HT, United Kingdom"}]},{"given":"Katrin","family":"Kuhls","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Mikrobiologie und Hygiene, Universita\u0308tsmedizin Berlin, Dorotheenstr. 96, Berlin 10117, Germany"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2008.09.005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-8-183"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2009.02.001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000080689"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2006.02.003"},{"key":"e_1_3_2_7_2","first-page":"233","volume":"19","year":"1967","unstructured":"Cavalli-Sforza, L. 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