{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:23:11Z","timestamp":1762100591911},"reference-count":28,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T00:00:00Z","timestamp":1164931200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This report describes application of PCR fingerprinting to identify common species of dermatophytes using the microsatellite primers M13, (GACA)<jats:sub>4<\/jats:sub>, and (GTG)<jats:sub>5<\/jats:sub>. The initial PCR analysis rendered a specific DNA fragment for<jats:italic>Microsporum audouinii<\/jats:italic>, which was cloned and sequenced. Based on the sequencing data of this fragment, forward (MA_1F) and reverse (MA_1R) primers were designed and verified by PCR to establish their reliability in the diagnosis of<jats:italic>M. audouinii<\/jats:italic>. These primers produced a singular PCR band of 431 bp specific only to strains and isolates of<jats:italic>M. audouinii<\/jats:italic>, based on a global test of 182 strains\/isolates belonging to 11 species of dermatophytes. These findings indicate these primers are reliable for diagnostic purposes, and we recommend their use in laboratory analysis.<\/jats:p>","DOI":"10.1128\/jcm.00759-06","type":"journal-article","created":{"date-parts":[[2006,12,6]],"date-time":"2006-12-06T18:54:05Z","timestamp":1165431245000},"page":"4336-4341","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Specific Primers for Rapid Detection of<i>Microsporum audouinii<\/i>by PCR in Clinical Samples"],"prefix":"10.1128","volume":"44","author":[{"given":"H. 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