{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:05:45Z","timestamp":1769040345820,"version":"3.49.0"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Streptococcus canis<\/jats:named-content>is an animal pathogen that occasionally causes human infections. Isolates recovered from infections of animals (<jats:italic>n<\/jats:italic>= 78, recovered from 2000 to 2010 in three European countries, mainly from house pets) and humans (<jats:italic>n<\/jats:italic>= 7, recovered from 2006 to 2010 in Portugal) were identified by phenotypic and genotypic methods and characterized by antimicrobial susceptibility testing, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and<jats:italic>emm<\/jats:italic>typing.<jats:named-content content-type=\"genus-species\">S. canis<\/jats:named-content>isolates presented considerable variability in biochemical profiles and 16S rRNA. Resistance to antimicrobial agents was low, with the most significant being<jats:italic>tet<\/jats:italic>(M)- and<jats:italic>tet<\/jats:italic>(O)-mediated tetracycline resistance. MLST analysis revealed a polyclonal structure of the<jats:named-content content-type=\"genus-species\">S. canis<\/jats:named-content>population causing infections, where the same genetic lineages were found infecting house pets and humans and were disseminated in distinct geographic locations. Phylogenetic analysis indicated that<jats:named-content content-type=\"genus-species\">S. canis<\/jats:named-content>was a divergent taxon of the sister species<jats:named-content content-type=\"genus-species\">Streptococcus pyogenes<\/jats:named-content>and<jats:named-content content-type=\"genus-species\">Streptococcus dysgalactiae<\/jats:named-content>subsp.<jats:italic>equisimilis<\/jats:italic>and found evidence of acquisition of genetic material by<jats:named-content content-type=\"genus-species\">S. canis<\/jats:named-content>from<jats:named-content content-type=\"genus-species\">S. dysgalactiae<\/jats:named-content>subsp.<jats:italic>equisimilis<\/jats:italic>. PFGE confirmed the MLST findings, further strengthening the similarity between animal and human isolates. The presence of<jats:italic>emm<\/jats:italic>-like genes was restricted to a few isolates and correlated with some MLST-based genetic lineages, but none of the human isolates could be<jats:italic>emm<\/jats:italic>typed. Our data show that<jats:named-content content-type=\"genus-species\">S. canis<\/jats:named-content>isolates recovered from house pets and humans constitute a single population and demonstrate that isolates belonging to the main genetic lineages identified have the ability to infect the human host, providing strong evidence for the zoonotic nature of<jats:named-content content-type=\"genus-species\">S. canis<\/jats:named-content>infection.<\/jats:p>","DOI":"10.1128\/jcm.02912-12","type":"journal-article","created":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T09:51:49Z","timestamp":1359021109000},"page":"1099-1109","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Multilocus Sequence Analysis of Streptococcus canis Confirms the Zoonotic Origin of Human Infections and Reveals Genetic Exchange with Streptococcus dysgalactiae subsp.<i>equisimilis<\/i>"],"prefix":"10.1128","volume":"51","author":[{"given":"M. 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