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The isolates were characterized by antimicrobial susceptibility, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and surface protein gene profiling. GBS invasive cases were found more frequently among men in all age groups. In addition, serotype Ia was the most frequent in our collection, whereas serotype V is dominant elsewhere. Serotype Ia was represented mainly by a single PFGE cluster defined by sequence type 23 (ST23) and surface protein gene<jats:italic>eps<\/jats:italic>and by ST24 and<jats:italic>bca<\/jats:italic>, similarly to neonatal invasive infections in Portugal, indicating that the same genetic lineages can be responsible for both vaginal colonization and invasive disease in all age groups. In contrast, the hypervirulent serotype III\/ST17 neonatal lineage was responsible for a minority of infections. Serotype V isolates were distributed into two genetic lineages, one defined by ST1 and surface protein gene<jats:italic>alp3<\/jats:italic>and macrolide resistant, and another presenting with ST2 and<jats:italic>eps<\/jats:italic>and fully susceptible to all antimicrobials tested. The<jats:italic>erm<\/jats:italic>(TR) gene was the most frequently found among erythromycin-resistant isolates, while the bovine-associated<jats:italic>tet<\/jats:italic>(O) gene was found in a minority of tetracycline-resistant isolates. Our data emphasize the importance of local identification of the genetic lineages responsible for GBS invasive infections in nonpregnant adults. The dominance of serotype Ia in invasive disease in Portugal highlights the importance of this serotype in GBS pathogenesis.<\/jats:p>","DOI":"10.1128\/jcm.05488-11","type":"journal-article","created":{"date-parts":[[2012,1,6]],"date-time":"2012-01-06T00:57:36Z","timestamp":1325811456000},"page":"1219-1227","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["Dominance of Serotype Ia among Group B Streptococci Causing Invasive Infections in Nonpregnant Adults in Portugal"],"prefix":"10.1128","volume":"50","author":[{"given":"E. 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