{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:59:43Z","timestamp":1753880383148,"version":"3.41.2"},"reference-count":14,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T00:00:00Z","timestamp":1702512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T00:00:00Z","timestamp":1702512000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Microbiol Biol Educ."],"published-print":{"date-parts":[[2023,12,14]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Shake flask cultivation is a routine technique in microbiology and biotechnology laboratories where cell growth can be affected by the hydrodynamic conditions, which depend on the agitation velocity, shaking diameter, and shake flask size. Liquid agitation is implemented inherently to increase aeration, substrate transfer to the cells, and prevent sedimentation, disregarding the role of hydrodynamics in microbial growth and metabolism. Here, we present a simple approach to help standardize the hydrodynamic forces in orbital shakers to increase the experimental accuracy and reproducibility and give students a better knowledge of the significance of the agitation process in microbial growth.<\/jats:p>","DOI":"10.1128\/jmbe.00099-23","type":"journal-article","created":{"date-parts":[[2023,9,19]],"date-time":"2023-09-19T13:02:41Z","timestamp":1695128561000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks"],"prefix":"10.1128","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4893-1985","authenticated-orcid":false,"given":"L\u00facia Chaves","family":"Sim\u00f5es","sequence":"first","affiliation":[{"name":"Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga, Portugal"},{"name":"LABBELS-Associate Laboratory, University of Minho, Braga\/Guimar\u00e3es, Portugal"}]},{"given":"Isabel","family":"Oliveira","sequence":"additional","affiliation":[{"name":"LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s\/n, Porto, Portugal"},{"name":"Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, Porto, Portugal"}]},{"given":"Anabela","family":"Borges","sequence":"additional","affiliation":[{"name":"LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s\/n, Porto, Portugal"},{"name":"Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, Porto, Portugal"}]},{"given":"In\u00eas Bezerra","family":"Gomes","sequence":"additional","affiliation":[{"name":"LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s\/n, Porto, Portugal"},{"name":"Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3355-4398","authenticated-orcid":false,"given":"Manuel","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s\/n, Porto, Portugal"},{"name":"Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, Porto, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00908-10"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1369-703x(00)00106-6"},{"key":"e_1_3_3_4_2","volume-title":"Microbiology: an introduction","author":"Tortora GJ","year":"2016","unstructured":"Tortora GJ, Funke BR, Case CL. 2016. 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