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All phage infections start with the binding of the viral particle to a specific receptor molecule on the host cell surface. Due to its importance in phage infections, this first step is of interest to many phage-related research and applications. However, many phage receptors are difficult to isolate. Styrene maleic acid lipid particles (SMALPs) are a recently developed approach to isolate membrane proteins in their native environment. In this study, we explore SMALPs as a tool to study phage-receptor interactions. We find that different phage species bind to SMALPs, while maintaining specificity to their receptor. We then characterize the time and concentration dependence of phage-SMALP interactions and furthermore show that they lead to genome ejection by the phage. The results presented here show that SMALPs are a useful tool for future studies of phage-receptor interactions.<\/jats:p>","DOI":"10.1128\/jvi.01559-20","type":"journal-article","created":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T14:35:45Z","timestamp":1600094145000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Development of Styrene Maleic Acid Lipid Particles as a Tool for Studies of Phage-Host Interactions"],"prefix":"10.1128","volume":"94","author":[{"given":"Patrick A.","family":"de Jonge","sequence":"first","affiliation":[{"name":"Theoretical Biology and Bioinformatics, Science4Life, Utrecht University, Utrecht, The Netherlands"},{"name":"Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, The Netherlands"}]},{"given":"Dieuwke J. 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