{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T08:42:09Z","timestamp":1774600929544,"version":"3.50.1"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"22","license":[{"start":{"date-parts":[[2010,11,15]],"date-time":"2010-11-15T00:00:00Z","timestamp":1289779200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2010,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Mutations in the receptor-binding site of the hemagglutinin of pandemic influenza A(H1N1) 2009 viruses have been detected sporadically. An Asp222Gly (D222G) substitution has been associated with severe or fatal disease. Here we show that 222G variants infected a higher proportion of ciliated cells in cultures of human airway epithelium than did viruses with 222D or 222E, which targeted mainly nonciliated cells. Carbohydrate microarray analyses showed that 222G variants bind a broader range of \u03b12-3-linked sialyl receptor sequences of a type expressed on ciliated bronchial epithelial cells and on epithelia within the lung. These features of 222G mutants may contribute to exacerbation of disease.<\/jats:p>","DOI":"10.1128\/jvi.01639-10","type":"journal-article","created":{"date-parts":[[2010,9,9]],"date-time":"2010-09-09T02:05:40Z","timestamp":1283997940000},"page":"12069-12074","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":177,"title":["Altered Receptor Specificity and Cell Tropism of D222G Hemagglutinin Mutants Isolated from Fatal Cases of Pandemic A(H1N1) 2009 Influenza Virus"],"prefix":"10.1128","volume":"84","author":[{"given":"Yan","family":"Liu","sequence":"first","affiliation":[{"name":"The Glycosciences Laboratory, Department of Medicine, Imperial College London, Northwick Park and St. Mark's Campus, Harrow, Middlesex HA1 3UJ, United Kingdom"}]},{"given":"Robert A.","family":"Childs","sequence":"additional","affiliation":[{"name":"The Glycosciences Laboratory, Department of Medicine, Imperial College London, Northwick Park and St. Mark's Campus, Harrow, Middlesex HA1 3UJ, United Kingdom"}]},{"given":"Tatyana","family":"Matrosovich","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University, Hans-Meerwein-Str. 2, 35043 Marburg, Germany"}]},{"given":"Stephen","family":"Wharton","sequence":"additional","affiliation":[{"name":"Division of Virology, MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom"}]},{"given":"Angelina S.","family":"Palma","sequence":"additional","affiliation":[{"name":"REQUIMTE, Centro de Qui\u0301mica Fina e Biotecnologia, Departamento de Qui\u0301mica, Faculdade de Cie\u0302ncias e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Wengang","family":"Chai","sequence":"additional","affiliation":[{"name":"The Glycosciences Laboratory, Department of Medicine, Imperial College London, Northwick Park and St. Mark's Campus, Harrow, Middlesex HA1 3UJ, United Kingdom"}]},{"given":"Rodney","family":"Daniels","sequence":"additional","affiliation":[{"name":"Division of Virology, MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom"}]},{"given":"Victoria","family":"Gregory","sequence":"additional","affiliation":[{"name":"Division of Virology, MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom"}]},{"given":"Jennifer","family":"Uhlendorff","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University, Hans-Meerwein-Str. 2, 35043 Marburg, Germany"}]},{"given":"Makoto","family":"Kiso","sequence":"additional","affiliation":[{"name":"Department of Applied Bioorganic Chemistry, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu-shi, Gifu, Japan"}]},{"given":"Hans-Dieter","family":"Klenk","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University, Hans-Meerwein-Str. 2, 35043 Marburg, Germany"}]},{"given":"Alan","family":"Hay","sequence":"additional","affiliation":[{"name":"Division of Virology, MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom"}]},{"given":"Ten","family":"Feizi","sequence":"additional","affiliation":[{"name":"The Glycosciences Laboratory, Department of Medicine, Imperial College London, Northwick Park and St. Mark's Campus, Harrow, Middlesex HA1 3UJ, United Kingdom"}]},{"given":"Mikhail","family":"Matrosovich","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University, Hans-Meerwein-Str. 2, 35043 Marburg, Germany"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"21","volume":"85","year":"2010","unstructured":"Anonymous. 2010. Preliminary review of D222G amino acid substitution in the haemagglutinin of pandemic influenza A (H1N1) 2009 viruses. Wkly. Epidemiol. Rec.85:21-22.","journal-title":"Wkly. Epidemiol. Rec."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1000449"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/652661"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0909-797"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1999.9732"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-5-85"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.06.002"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02677-05"},{"key":"e_1_3_2_10_2","first-page":"6","volume":"15","year":"2010","unstructured":"Kilander, A., R. Rykkvin, S. G. Dudman, and O. Hungnes. 2010. Observed association between the HA1 mutation D222G in the 2009 pandemic influenza A(H1N1) virus and severe clinical outcome, Norway 2009-2010. Eurosurveillance15:6-8.","journal-title":"Eurosurveillance"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2006.11.003"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.4.1285-1289.1989"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.10.4015-4023.1992"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)81495-3"},{"key":"e_1_3_2_15_2","first-page":"15","volume":"15","year":"2010","unstructured":"Mak, G. C., K. W. Au, L. S. Tai, K. C. Chuang, K. C. Cheng, T. C. Shiu, and W. Lim. 2010. Association of D222G substitution in haemagglutinin of 2009 pandemic influenza A (H1N1) with severe disease. Eurosurveillance15:15-16.","journal-title":"Eurosurveillance"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.18.8502-8512.2000"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1159\/000151617"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308001101"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1605.091858"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0910.020789"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/440435a"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.11.002"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00384-06"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.070248"},{"key":"e_1_3_2_25_2","first-page":"RRN1152","volume":"22","year":"2010","unstructured":"Yang, H., P. Carney, and J. Stevens. 2010. 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