{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T00:54:51Z","timestamp":1718585691064},"reference-count":31,"publisher":"American Society for Microbiology","issue":"15","license":[{"start":{"date-parts":[[2008,8,1]],"date-time":"2008-08-01T00:00:00Z","timestamp":1217548800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>CD8 T cells play a major role in antiviral immune responses. Their importance for progression to chronic hepatitis C and response to treatment are still unclear. To address these issues, hepatitis C virus (HCV)-specific CD8 T-cell responses were monitored, at the single-cell level, using HLA class I pentamers specific for HCV core and HCV NS3 epitopes, in 23 chronically infected patients during treatment with pegylated alpha interferon and ribavirin. Patients who presented a sustained-response to therapy had stronger HCV-specific CD8 T-cell responses at all time points studied. Moreover, there were clear differences in the phenotypes of these cells during therapy: in responder patients, terminally differentiated effector cells increased more rapidly, and their frequency was always higher than in nonresponder patients. Sustained-responder patients also showed a higher frequency of HCV-specific CD8 T cells producing cytotoxic factors. Overall, a late and inefficient differentiation process of HCV-specific CD8 T cells might be associated with lack of response to treatment. A better knowledge of the mechanisms underlying this impairment may be important for the development of new therapeutic strategies to maintain, restore, or increase CD8 T-cell effectiveness in chronic HCV infection.<\/jats:p>","DOI":"10.1128\/jvi.02175-07","type":"journal-article","created":{"date-parts":[[2008,5,15]],"date-time":"2008-05-15T01:39:34Z","timestamp":1210815574000},"page":"7567-7577","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Differences in Hepatitis C Virus (HCV)-Specific CD8 T-Cell Phenotype during Pegylated Alpha Interferon and Ribavirin Treatment Are Related to Response to Antiviral Therapy in Patients Chronically Infected with HCV"],"prefix":"10.1128","volume":"82","author":[{"given":"Joana","family":"Caetano","sequence":"first","affiliation":[{"name":"Molecular Biology Laboratory, Histocompatibility Center of Coimbra, Coimbra, Portugal"}]},{"given":"Anto\u0301nio","family":"Martinho","sequence":"additional","affiliation":[{"name":"Molecular Biology Laboratory, Histocompatibility Center of Coimbra, Coimbra, Portugal"}]},{"given":"Artur","family":"Paiva","sequence":"additional","affiliation":[{"name":"Flow Cytometry Laboratory, Histocompatibility Center of Coimbra, Coimbra, Portugal"}]},{"given":"Beatriz","family":"Pais","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Hospital Center of Coimbra, Coimbra, Portugal"}]},{"given":"Cristina","family":"Valente","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Hospital Center of Coimbra, Coimbra, Portugal"}]},{"given":"Cristina","family":"Luxo","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"S135","volume":"38","year":"2003","unstructured":"Alberti, A., and L. 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