{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T23:09:13Z","timestamp":1776726553643,"version":"3.51.2"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2019,8,20]],"date-time":"2019-08-20T00:00:00Z","timestamp":1566259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T00:00:00Z","timestamp":1566864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"COMPETE2020","award":["LISBOA-01-0145-FEDER-007660"],"award-info":[{"award-number":["LISBOA-01-0145-FEDER-007660"]}]},{"name":"Green-IT","award":["UID\/Multi\/04551\/2013"],"award-info":[{"award-number":["UID\/Multi\/04551\/2013"]}]},{"name":"European Union's Horizon 2020","award":["No 810856"],"award-info":[{"award-number":["No 810856"]}]},{"name":"COMPETE2020","award":["Project No 022161"],"award-info":[{"award-number":["Project No 022161"]}]},{"name":"FCT Portugal","award":["SFRH\/BPD\/96952\/2013"],"award-info":[{"award-number":["SFRH\/BPD\/96952\/2013"]}]},{"name":"FCT Portugal","award":["PTDC\/BBB-BQB\/4178\/2014"],"award-info":[{"award-number":["PTDC\/BBB-BQB\/4178\/2014"]}]},{"name":"FCT Portugal","award":["PTDC\/BIA-BQM\/30176\/2017"],"award-info":[{"award-number":["PTDC\/BIA-BQM\/30176\/2017"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2019,8,27]]},"abstract":"<jats:p>\n                    Thermophilic Gram-positive organisms were recently shown to be a promising class of organisms to be used in bioelectrochemical systems for the production of electrical energy. These organisms present a thick peptidoglycan layer that was thought to preclude them to perform extracellular electron transfer (i.e., exchange catabolic electrons with solid electron acceptors outside the cell). In this paper, we describe the structure and functional mechanisms of the multiheme cytochrome OcwA, the terminal reductase of the Gram-positive bacterium\n                    <jats:italic>Thermincola potens<\/jats:italic>\n                    JR found at the cell surface of this organism. The results presented here show that this protein can take the role of a respiratory \u201cSwiss Army knife,\u201d allowing this organism to grow in environments with soluble and insoluble substrates. Moreover, it is shown that it is unrelated to terminal reductases found at the cell surface of other electroactive organisms. Instead, OcwA is similar to terminal reductases of soluble electron acceptors. Our data reveal that terminal oxidoreductases of soluble and insoluble substrates are evolutionarily related, providing novel insights into the evolutionary pathway of multiheme cytochromes.\n                  <\/jats:p>","DOI":"10.1128\/mbio.01210-19","type":"journal-article","created":{"date-parts":[[2019,8,19]],"date-time":"2019-08-19T05:31:11Z","timestamp":1566192671000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["How Thermophilic Gram-Positive Organisms Perform Extracellular Electron Transfer: Characterization of the Cell Surface Terminal Reductase OcwA"],"prefix":"10.1128","volume":"10","author":[{"given":"N. L.","family":"Costa","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade NOVA de Lisboa, Lisbon, Portugal"}]},{"given":"B.","family":"Hermann","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Biochemie, Albert-Ludwigs-Universit\u00e4t Freiburg, Freiburg im Breisgau, Germany"}]},{"given":"V.","family":"Fourmond","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, Centre National de la Recherche Scientifique (CNRS), Marseille, France"}]},{"given":"M. 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