{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T02:06:58Z","timestamp":1774490818348,"version":"3.50.1"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2025,7,29]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic toggle=\"yes\">Streptococcus pneumoniae<\/jats:italic>\n              (pneumococcus) is a leading cause of infections, particularly in infants and the elderly. Recent advances in molecular methods suggest higher pneumococcal carriage rates among adults than previously estimated, raising questions about their role in transmission. This study aimed to estimate pneumococcal carriage prevalence, identify circulating serotypes, and assess risk factors for colonization among adults during the COVID-19 pandemic in Portugal. We conducted a prospective observational study among civil servants aged \u226518 years in Oeiras Municipality from February 2021 to February 2022. Paired nasopharyngeal and oropharyngeal samples were analyzed using qPCR to detect pneumococcal carriage and 66 serotypes\/serogroups. This included novel primers and probes for serotypes 4 and 24B\/F, overcoming previous concerns associated with false positivity. Risk factors were identified using Bayesian adaptive sampling for variable selection in generalized linear model. Among 3,574 participants, 6.9% were pneumococcal carriers through qPCR without prior culture enrichment. Carriage rates were higher in oropharyngeal than nasopharyngeal samples (5.3% vs 3.7%,\n              <jats:italic toggle=\"yes\">P<\/jats:italic>\n              &lt; 0.001). Twenty-six serotypes\/serogroups were identified, with the most common being non-encapsulated (NT), 10A, 23B, 3, 11A\/D, 33A\/F\/37, 16F, and 31. Excluding NT, the most frequent serotypes collectively accounted for 45.3% of all carriers. Vaccine coverage estimates were 13.5% for PCV13, 20.4% for PCV15, 40.0% for PCV20, and 64.1% for PCV21. Contact with children &lt; 18 years increased the odds of colonization by 2.73-fold (95% confidence interval [CI], 2.01\u20133.75), while being male reduced the odds by 54% (odds ratio = 0.46; 95% CI, 0.30\u20130.69). These findings emphasize the need for ongoing surveillance to clarify adults\u2019 role in pneumococcal transmission and support prevention strategies, including adult vaccination and community-level interventions, to mitigate pneumococcal disease.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                <jats:italic toggle=\"yes\">Streptococcus pneumoniae<\/jats:italic>\n                is a major pathogen causing significant disease worldwide, yet adult carriage remains underexplored. By evaluating pneumococcal carriage among adults in Portugal during the COVID-19 pandemic, this study provides critical insights into circulating serotypes, including those not targeted by 13-valent pneumococcal conjugate vaccine (PCV13), and highlights key risk factors such as contact with children and sex differences. The findings reveal substantial potential coverage for newer PCVs. This work underscores the importance of adult-focused prevention strategies, including vaccination and ongoing surveillance, to reduce pneumococcal transmission and disease burden in the community.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00082-25","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T09:01:02Z","timestamp":1749546062000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["<i>Streptococcus pneumoniae<\/i>\n            carriage in adults during the COVID-19 pandemic in Portugal: dominance of serotypes included in broader PCVs and of serotype 3"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8143-5254","authenticated-orcid":false,"given":"S\u00f3nia T.","family":"Almeida","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Microbiology of Human Pathogens, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa","place":["Oeiras, Portugal"]}]},{"given":"A. Cristina","family":"Paulo","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology of Human Pathogens, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa","place":["Oeiras, Portugal"]}]},{"given":"Alexandra S.","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology of Human Pathogens, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa","place":["Oeiras, Portugal"]}]},{"given":"B\u00e1rbara","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology of Human Pathogens, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa","place":["Oeiras, Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9804-827X","authenticated-orcid":false,"given":"Raquel","family":"S\u00e1-Le\u00e3o","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology of Human Pathogens, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa","place":["Oeiras, Portugal"]}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0001-8"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01366"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00024-23"},{"key":"e_1_3_4_5_2","unstructured":"CDC. 2023. 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Persistence of PCV13 serotypes and the importance of serotype 8 in adult invasive pneumococcal disease in Portugal (2019-2023). 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