{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:44:03Z","timestamp":1753883043898,"version":"3.41.2"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100008530","name":"Fundo Europeu de Desenvolvimento Regional","doi-asserted-by":"crossref","award":["LISBOA-01-0145-FEDER- 016417"],"award-info":[{"award-number":["LISBOA-01-0145-FEDER- 016417"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001871","name":"MEC | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BIA-MIC\/31645\/2017"],"award-info":[{"award-number":["PTDC\/BIA-MIC\/31645\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"MEC | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/CVT-CVT\/29510\/2017"],"award-info":[{"award-number":["PTDC\/CVT-CVT\/29510\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"MEC | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/04378\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"MEC | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"MEC | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["LA\/P\/0140\/2020"],"award-info":[{"award-number":["LA\/P\/0140\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fundo Europeu de Desenvolvimento Regional","doi-asserted-by":"crossref","award":["LISBOA-01-0145-FEDER007660"],"award-info":[{"award-number":["LISBOA-01-0145-FEDER007660"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008530","name":"Fundo Europeu de Desenvolvimento Regional","doi-asserted-by":"crossref","award":["UID\/Multi\/04378\/2019"],"award-info":[{"award-number":["UID\/Multi\/04378\/2019"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2022,10,26]]},"abstract":"<jats:p>\n            The cell wall of bacteria, namely, peptidoglycan, is the target of several antibiotic classes such as \u03b2-lactams.\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            is well known for its capacity to adapt to antibiotic stress and develop resistance strategies, namely, to \u03b2-lactams. In this context, the construction of cell wall mutants provides useful models to study the development of such resistance mechanisms.\n          <\/jats:p>","DOI":"10.1128\/spectrum.02483-21","type":"journal-article","created":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T13:03:09Z","timestamp":1662987789000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of a Cell Wall Mutant Highlights Rho-Dependent Genome Amplification Events in Staphylococcus aureus"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5559-9554","authenticated-orcid":true,"given":"Raquel","family":"Portela","sequence":"first","affiliation":[{"name":"Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, Caparica, Portugal"},{"name":"Laboratory of Molecular Microbiology of Bacterial Pathogens, UCIBIO \u2013 Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, Caparica, Portugal"}]},{"given":"Nuno","family":"A. Faria","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Evolution and Molecular Epidemiology, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal"}]},{"given":"Michael","family":"Mwangi","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology and Infectious Diseases, The Rockefeller University, New York, New York, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1323-7184","authenticated-orcid":true,"given":"Maria","family":"Miragaia","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Evolution and Molecular Epidemiology, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal"}]},{"given":"Herm\u00ednia","family":"de Lencastre","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology and Infectious Diseases, The Rockefeller University, New York, New York, USA"},{"name":"Laboratory of Molecular Genetics, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal"}]},{"given":"Alexander","family":"Tomasz","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology and Infectious Diseases, The Rockefeller University, New York, New York, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4533-7531","authenticated-orcid":true,"given":"Rita","family":"Gon\u00e7alves Sobral","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, Caparica, Portugal"},{"name":"Laboratory of Molecular Microbiology of Bacterial Pathogens, UCIBIO \u2013 Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, Caparica, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/107662903322286436"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.7.2543-2553.2006"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00512-9"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060815-014844"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.4.1099-1103.2001"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1009564108"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.17010011"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2014.03.023"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.09.053"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3316"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2016.05.012"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1152763"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1206848"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1097-2765(00)80476-1"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00537-12"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00044-07"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2014.0042"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)49904-3"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.08.036"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00869-17"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.06.003"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.4161\/trns.25101"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1213123110"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000244"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab093"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.3791\/253"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02199440"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.15.4952-4959.1992"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02026129"},{"key":"e_1_3_3_31_2","unstructured":"Machado MP Halkilahti J Mendes I Pinto M Lizarazo E Gomes JP Ramirez M Rossi M Carrico JC. n.d. INNUca. https:\/\/github.com\/B-UMMI\/INNUca."},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104984"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00957-19"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02483-21","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02483-21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T14:04:13Z","timestamp":1666793053000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.02483-21"}},"subtitle":[],"editor":[{"given":"Joanna B.","family":"Goldberg","sequence":"additional","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2022,10,26]]},"references-count":32,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,10,26]]}},"alternative-id":["10.1128\/spectrum.02483-21"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.02483-21","relation":{},"ISSN":["2165-0497"],"issn-type":[{"type":"electronic","value":"2165-0497"}],"subject":[],"published":{"date-parts":[[2022,10,26]]},"assertion":[{"value":"2021-12-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02483-21"}}