{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T21:45:34Z","timestamp":1768340734345,"version":"3.49.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T00:00:00Z","timestamp":1681344000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T00:00:00Z","timestamp":1681344000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001871","name":"MEC | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/50026\/2020"],"award-info":[{"award-number":["UIDB\/50026\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"MEC | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/50026\/2020"],"award-info":[{"award-number":["UIDP\/50026\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"MEC | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/03348\/2022"],"award-info":[{"award-number":["PTDC\/03348\/2022"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"MEC | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["CEECIND\/00185\/2020"],"award-info":[{"award-number":["CEECIND\/00185\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"MEC | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["CEECIND\/03070\/2020"],"award-info":[{"award-number":["CEECIND\/03070\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"MEC | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["CEECIND\/04058\/2018"],"award-info":[{"award-number":["CEECIND\/04058\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2023,4,13]]},"abstract":"<jats:p>Sporotrichosis is the most prevalent subcutaneous mycosis worldwide and has recently become a public health concern. Although immunocompetent hosts are also prone to sporotrichosis, immunodeficient hosts often develop a more severe and disseminated form of disease.<\/jats:p>","DOI":"10.1128\/spectrum.04564-22","type":"journal-article","created":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T14:01:58Z","timestamp":1677506518000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Development of a Versatile Toolbox for Genetic Manipulation of\n            <i>Sporothrix brasiliensis<\/i>"],"prefix":"10.1128","volume":"11","author":[{"given":"Matheus","family":"Tavares","sequence":"first","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B's-PT Government Associate Laboratory, Guimar\u00e3es\/Braga, Portugal"}]},{"given":"Jorge Carlos","family":"Sousa-Filho","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B's-PT Government Associate Laboratory, Guimar\u00e3es\/Braga, Portugal"}]},{"given":"Ian Alves","family":"Machado","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B's-PT Government Associate Laboratory, Guimar\u00e3es\/Braga, Portugal"}]},{"given":"Relber Aguiar","family":"Gon\u00e7ales","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B's-PT Government Associate Laboratory, Guimar\u00e3es\/Braga, Portugal"}]},{"given":"Daniela","family":"Antunes","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B's-PT Government Associate Laboratory, Guimar\u00e3es\/Braga, Portugal"}]},{"given":"Ana","family":"Mendes-Frias","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B's-PT Government Associate Laboratory, Guimar\u00e3es\/Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9270-2717","authenticated-orcid":true,"given":"Ricardo","family":"Silvestre","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B's-PT Government Associate Laboratory, Guimar\u00e3es\/Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8935-8030","authenticated-orcid":true,"given":"Agostinho","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B's-PT Government Associate Laboratory, Guimar\u00e3es\/Braga, Portugal"}]},{"given":"Eg\u00eddio","family":"Torrado","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B's-PT Government Associate Laboratory, Guimar\u00e3es\/Braga, Portugal"}]},{"given":"Cristina","family":"Cunha","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B's-PT Government Associate Laboratory, Guimar\u00e3es\/Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8436-9398","authenticated-orcid":true,"given":"Fernando","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B's-PT Government Associate Laboratory, Guimar\u00e3es\/Braga, Portugal"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-020-00425-0"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myx103"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1590\/abd1806-4841.2017279"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/fov065"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2007.04.004"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00412-18"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-539-8_4"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.funbio.2018.07.004"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2005.10.004"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2011.01.017"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-005-0578-0"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.4148\/1941-4765.1082"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00284"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiaa707"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-943"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-019-1167-0"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-020-01060-4"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-019-0667-6"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1080\/15459620500391650"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.REV119.009109"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.top64"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1590\/1678-4685-gmb-2021-0073"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.154"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.23.6852-6861.2001"},{"key":"e_1_3_3_26_2","volume-title":"From genes to genomes","author":"Dale JW","year":"2011","unstructured":"Dale JW, Von Schantz M, Plant ND. 2011. From genes to genomes, 3rd ed. Wiley-Blackwell, Hoboken, NJ.","edition":"3"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.565439"},{"key":"e_1_3_3_28_2","article-title":"Transcriptome-wide expression profiling of Sporothrix schenckii yeast and mycelial forms and the establishment of the Sporothrix Genome DataBase","volume":"6","author":"Giosa D","year":"2020","unstructured":"Giosa D, Felice MR, Giuffr\u00e8 L, Aiese Cigliano R, Paytuv\u00ed-Gallart A, Lo Passo C, Barresi C, D\u2019Alessandro E, Huang H, Criseo G, Mora-Montes HM, de Hoog S, Romeo O. 2020. Transcriptome-wide expression profiling of Sporothrix schenckii yeast and mycelial forms and the establishment of the Sporothrix Genome DataBase. Microb Genom 6.","journal-title":"Microb Genom"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.934227"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006169"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/ffunb.2022.833111"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2022.104555"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075656"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/lam.13286"},{"key":"e_1_3_3_35_2","volume-title":"Molecular cloning\u202f: a laboratory manual.","author":"Sambrook G","year":"2012","unstructured":"Sambrook G, Sambrook J, Joseph MR. 2012. Molecular cloning\u202f: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY."},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep27459"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-6-62"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1871-4_8"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2013.01.010"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.04564-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.04564-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T13:20:51Z","timestamp":1681392051000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.04564-22"}},"subtitle":[],"editor":[{"given":"Gustavo H.","family":"Goldman","sequence":"additional","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2023,4,13]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,4,13]]}},"alternative-id":["10.1128\/spectrum.04564-22"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.04564-22","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,13]]},"assertion":[{"value":"2022-11-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e04564-22"}}