{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:07:13Z","timestamp":1773659233030,"version":"3.50.1"},"reference-count":22,"publisher":"Pleiades Publishing Ltd","issue":"10","license":[{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Biochemistry Moscow"],"published-print":{"date-parts":[[2021,10]]},"DOI":"10.1134\/s0006297921100096","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T10:03:09Z","timestamp":1634724189000},"page":"1275-1287","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development of a Platform for Producing Recombinant Protein Components of Epitope Vaccines for the Prevention of COVID-19"],"prefix":"10.1134","volume":"86","author":[{"given":"Anna S.","family":"Karyagina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander V.","family":"Gromov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatyana M.","family":"Grunina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander M.","family":"Lyaschuk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander V.","family":"Grishin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia V.","family":"Strukova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria S.","family":"Generalova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoya M.","family":"Galushkina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lyubov\u2019 A.","family":"Soboleva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga Yu.","family":"Dobrinina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatyana N.","family":"Bolshakova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina E.","family":"Subbotina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekaterina A.","family":"Romanovskaya-Romanko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor V.","family":"Krasilnikov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikita B.","family":"Polyakov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrey I.","family":"Solovyev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniil A.","family":"Grumov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir G.","family":"Zhukhovitsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekaterina I.","family":"Ryabova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir V.","family":"Prokofiev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir G.","family":"Lunin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"137","published-online":{"date-parts":[[2021,10,20]]},"reference":[{"key":"2235_CR1","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1038\/nri891","volume":"9","author":"D. R. Burton","year":"2002","unstructured":"Burton, D. R. (2002) Antibodies, viruses and vaccines, Nat. Rev. Immunol., 9, 706-713, \nhttps:\/\/doi.org\/10.1038\/nri891.","journal-title":"Nat. Rev. Immunol."},{"key":"2235_CR2","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.coi.2016.05.012","volume":"42","author":"K. E. Neu","year":"2016","unstructured":"Neu, K. E., Henry Dunand, C. J., and Wilson, P. C. (2016) Heads, stalks and everything else: how can antibodies eradicate influenza as a human disease? Curr. Opin. Immunol., 42, 48-55, \nhttps:\/\/doi.org\/10.1016\/j.coi.2016.05.012.","journal-title":"Curr. Opin. Immunol."},{"key":"2235_CR3","doi-asserted-by":"publisher","first-page":"1179","DOI":"10.1038\/s41590-018-0235-7","volume":"19","author":"D. Sok","year":"2018","unstructured":"Sok, D., and Burton, D. R. (2018) Recent progress in broadly neutralizing antibodies to HIV, Nat. Immunol., 19, 1179-1188, \nhttps:\/\/doi.org\/10.1038\/s41590-018-0235-7.","journal-title":"Nat. Immunol."},{"key":"2235_CR4","doi-asserted-by":"publisher","first-page":"1102","DOI":"10.1038\/ni.3515","volume":"17","author":"W. Dejnirattisai","year":"2016","unstructured":"Dejnirattisai, W., Supasa, P., Wongwiwat, W., Rouvinski, A., Barba-Spaeth, G., et al. (2016) Dengue virus sero-cross-reactivity drives antibody-dependent enhancement of infection with zika virus, Nat. Immunol., 17, 1102-1108, \nhttps:\/\/doi.org\/10.1038\/ni.3515.","journal-title":"Nat. Immunol."},{"key":"2235_CR5","doi-asserted-by":"publisher","first-page":"1119","DOI":"10.1126\/science.abd2321","volume":"369","author":"M. Yuan","year":"2020","unstructured":"Yuan, M., Liu, H., Wu, N. C., Lee, C. D., Zhu, X., et al. (2020) Structural basis of a shared antibody response to SARS\u2011CoV\u20112, Science, 369, 1119-1123, \nhttps:\/\/doi.org\/10.1126\/science.abd2321.","journal-title":"Science"},{"key":"2235_CR6","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1038\/s41591-018-0042-6","volume":"24","author":"K. Xu","year":"2018","unstructured":"Xu, K., Acharya, P., Kong, R., Cheng, C., Chuang, G. Y., et al. (2018) Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1, Nat. Med., 24, 857-867, \nhttps:\/\/doi.org\/10.1038\/s41591-018-0042-6.","journal-title":"Nat. Med."},{"key":"2235_CR7","doi-asserted-by":"publisher","first-page":"108666","DOI":"10.1016\/j.celrep.2020.108666","volume":"34","author":"S. Lu","year":"2021","unstructured":"Lu, S., Xie, X. X., Zhao, L., Wang, B., Zhu, J., et al. (2021) The immunodominant and neutralization linear epitopes for SARS\u2011CoV\u20112, Cell Rep., 34, 108666, \nhttps:\/\/doi.org\/10.1016\/j.celrep.2020.108666.","journal-title":"Cell Rep."},{"key":"2235_CR8","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1093\/protein\/gzl005","volume":"19","author":"S. M. Juraja","year":"2006","unstructured":"Juraja, S. M., Mulhern, T. D., Hudson, P. J., Hattarki, M. K., Carmichael, J. A., and Nuttall, S. D. (2006) Engineering of the Escherichia coli Im7 immunity protein as a loop display scaffold, Protein Eng. Des. Sel., 19, 231-244, \nhttps:\/\/doi.org\/10.1093\/protein\/gzl005.","journal-title":"Protein Eng. Des. Sel."},{"key":"2235_CR9","doi-asserted-by":"publisher","first-page":"2856","DOI":"10.1038\/nprot.2006.468","volume":"1","author":"A. Shevchenko","year":"2006","unstructured":"Shevchenko, A., Tomas, H., Havlis, J., Olsen, J. V., and Mann, M. (2006) In-gel digestion for mass spectrometric characterization of proteins and proteomes, Nat. Protoc., 1, 2856-2860, \nhttps:\/\/doi.org\/10.1038\/nprot.2006.468.","journal-title":"Nat. Protoc."},{"key":"2235_CR10","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1134\/S0006297917110062","volume":"82","author":"T. M. Grunina","year":"2017","unstructured":"Grunina, T. M., Demidenko, A. V., Lyaschuk, A. M., Poponova, M. S., Galushkina, Z. M., et al. (2017) Recombinant human erythropoietin with additional processable protein domains: purification of protein synthesized in Escherichia coli heterologous expression system, Biochemistry (Moscow), 82, 1285-1294, \nhttps:\/\/doi.org\/10.1134\/S0006297917110062.","journal-title":"Biochemistry (Moscow)"},{"key":"2235_CR11","doi-asserted-by":"publisher","DOI":"10.21203\/rs.3.rs-59639\/v1","author":"S.-Y. Lee","year":"2020","unstructured":"Lee, S.-Y., Kim, C., Ryu, D.-K., Lee, J., Kim, Y.-I., et al. (2020) A novel neutralizing antibody targeting receptor binding domain of SARS\u2011CoV\u20112, Res. Square, preprint, \nhttps:\/\/doi.org\/10.21203\/rs.3.rs-59639\/v1.","journal-title":"Res. Square"},{"key":"2235_CR12","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1038\/s41541-021-00347-y","volume":"6","author":"A. Zuniga","year":"2021","unstructured":"Zuniga, A., Rassek, O., Vrohlings, M., Marrero-Nodarse, A., Moehle, K., et al. (2021) An epitope-specific chemically defined nanoparticle vaccine for respiratory syncytial virus, Vaccines, 6, 85, \nhttps:\/\/doi.org\/10.1038\/s41541-021-00347-y.","journal-title":"Vaccines"},{"key":"2235_CR13","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1126\/science.abf6840","volume":"371","author":"A. A. Cohen","year":"2021","unstructured":"Cohen, A. A., Gnanapragasam, P., Lee, Y. E., Hoffman, P. R., Ou, S., et al. (2021) Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice, Science, 371, 735-741, \nhttps:\/\/doi.org\/10.1126\/science.abf6840.","journal-title":"Science"},{"key":"2235_CR14","doi-asserted-by":"publisher","first-page":"2738","DOI":"10.1021\/acsnano.0c08379","volume":"15","author":"Y. F. Kang","year":"2021","unstructured":"Kang, Y. F., Sun, C., Zhuang, Z., Yuan, R. Y., Zheng, Q., et al. (2021) Rapid Development of SARS\u2011CoV\u20112 spike protein receptor-binding domain self-assembled nanoparticle vaccine candidates, ACS Nano, 15, 2738-2752, \nhttps:\/\/doi.org\/10.1021\/acsnano.0c08379.","journal-title":"ACS Nano"},{"key":"2235_CR15","doi-asserted-by":"publisher","first-page":"542","DOI":"10.1038\/s41467-020-20654-7","volume":"12","author":"T. K. Tan","year":"2021","unstructured":"Tan, T. K., Rijal, P., Rahikainen, R., Keeble, A. H., Schimanski, L., et al. (2021) A COVID\u201119 vaccine candidate using SpyCatcher multimerization of the SARS\u2011CoV\u20112 spike protein receptor-binding domain induces potent neutralising antibody responses, Nat. Commun., 12, 542, \nhttps:\/\/doi.org\/10.1038\/s41467-020-20654-7.","journal-title":"Nat. Commun."},{"key":"2235_CR16","doi-asserted-by":"publisher","first-page":"1934","DOI":"10.3390\/ijms22041934","volume":"22","author":"Q. D. Nguyen","year":"2021","unstructured":"Nguyen, Q. D., Kikuchi, K., Maity, B., and Ueno, T. (2021) The versatile manipulations of self-assembled proteins in vaccine design, Int. J. Mol. Sci., 22, 1934, \nhttps:\/\/doi.org\/10.3390\/ijms22041934.","journal-title":"Int. J. Mol. Sci."},{"key":"2235_CR17","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1089\/vim.2012.0076","volume":"26","author":"J. Li","year":"2013","unstructured":"Li, J., Ulitzky, L., Silberstein, E., Taylor, D. R., and Viscidi, R. (2013) Immunogenicity and protection efficacy of monomeric and trimeric recombinant SARS coronavirus spike protein subunit vaccine candidates, Viral Immunol., 26, 126-132, \nhttps:\/\/doi.org\/10.1089\/vim.2012.0076.","journal-title":"Viral Immunol."},{"key":"2235_CR18","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1016\/j.virol.2016.10.005","volume":"499","author":"W. Tai","year":"2016","unstructured":"Tai, W., Zhao, G., Sun, S., Guo, Y., Wang, Y., et al. (2016) A recombinant receptor-binding domain of MERS-CoV in trimeric form protects human dipeptidyl peptidase 4 (hDPP4) transgenic mice from MERS-CoV infection, Virology, 499, 375-382, \nhttps:\/\/doi.org\/10.1016\/j.virol.2016.10.005.","journal-title":"Virology"},{"key":"2235_CR19","doi-asserted-by":"publisher","first-page":"1014","DOI":"10.1126\/science.abd0831","volume":"369","author":"A. Baum","year":"2020","unstructured":"Baum, A., Fulton, B. O., Wloga, E., Copin, R., Pascal, K. E., et al. (2020) Antibody cocktail to SARS\u2011CoV\u20112 spike protein prevents rapid mutational escape seen with individual antibodies, Science, 369, 1014-1018, \nhttps:\/\/doi.org\/10.1126\/science.abd0831.","journal-title":"Science"},{"key":"2235_CR20","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1016\/j.chom.2020.11.007","volume":"29","author":"A. J. Greaney","year":"2021","unstructured":"Greaney, A. J., Starr, T. N., Gilchuk, P., Zost, S. J., Binshtein, E., et al. (2021) Complete mapping of mutations to the SARS\u2011CoV\u20112 spike receptor-binding domain that escape antibody recognition, Cell Host Microbe, 29, 44-57.e9, \nhttps:\/\/doi.org\/10.1016\/j.chom.2020.11.007.","journal-title":"Cell Host Microbe"},{"key":"2235_CR21","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1016\/j.chom.2021.02.003","volume":"29","author":"A. J. Greaney","year":"2021","unstructured":"Greaney, A. J., Loes, A. N., Crawford, K. H. D., Starr, T. N., Malone, K. D., et al. (2021) Comprehensive mapping of mutations in the SARS\u2011CoV\u20112 receptor-binding domain that affect recognition by polyclonal human plasma antibodies, Cell Host Microbe, 29, 463-476.e6, \nhttps:\/\/doi.org\/10.1016\/j.chom.2021.02.003.","journal-title":"Cell Host Microbe"},{"key":"2235_CR22","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1038\/s41591-021-01255-3","volume":"27","author":"H. Tegally","year":"2021","unstructured":"Tegally, H., Wilkinson, E., Lessells, R. J., Giandhari, J., Pillay, S., et al. (2021) Sixteen novel lineages of SARS\u2011CoV\u20112 in South Africa, Nat. Med., 27, 440-446, \nhttps:\/\/doi.org\/10.1038\/s41591-021-01255-3.","journal-title":"Nat. Med."}],"container-title":["Biochemistry (Moscow)"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1134\/S0006297921100096.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1134\/S0006297921100096","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1134\/S0006297921100096.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:11:44Z","timestamp":1773619904000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1134\/S0006297921100096"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10]]},"references-count":22,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["2235"],"URL":"https:\/\/doi.org\/10.1134\/s0006297921100096","relation":{},"ISSN":["0006-2979","1608-3040"],"issn-type":[{"value":"0006-2979","type":"print"},{"value":"1608-3040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10]]},"assertion":[{"value":"8 June 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 July 2021","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 July 2021","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 October 2021","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no conflict of interest in financial or any other sphere. All applicable international, national and\/or institutional guidelines for the care and use of animals have been followed.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics declarations"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}