{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T11:09:54Z","timestamp":1779880194225,"version":"3.53.1"},"reference-count":11,"publisher":"Pleiades Publishing Ltd","issue":"1","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Dokl Biochem Biophys"],"published-print":{"date-parts":[[2025,4]]},"DOI":"10.1134\/s1607672924601136","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T18:18:01Z","timestamp":1744395481000},"page":"157-159","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Optimization of A549 Cell Transfection Efficiency with a Plasmid Encoding the N-Protein of the SARS-CoV-2 Virus"],"prefix":"10.1134","volume":"521","author":[{"given":"Yu. V.","family":"Khramtsov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. N.","family":"Lupanova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. A.","family":"Rosenkranz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. P.","family":"Georgiev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. S.","family":"Sobolev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"137","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"key":"7665_CR1","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1016\/j.meegid.2007.07.004","volume":"8","author":"M. Surjit","year":"2008","unstructured":"Surjit, M. and Lal, S.K., Infect. Genet. Evol., 2008, vol.\u00a08, pp. 397\u2013405.","journal-title":"Infect. Genet. Evol"},{"key":"7665_CR2","unstructured":"Wu, C. and Zheng, M., Preprint 2020020247, 2020."},{"key":"7665_CR3","doi-asserted-by":"publisher","first-page":"56","DOI":"10.4103\/ijp.IJP_115_20","volume":"52","author":"M. Prajapat","year":"2020","unstructured":"Prajapat, M., Sarma, P., Shekhar, N., et al., Indian J. Pharmacol., 2020, vol. 52, p. 56.","journal-title":"Indian J. Pharmacol"},{"key":"7665_CR4","doi-asserted-by":"publisher","first-page":"1507","DOI":"10.3390\/v14071507","volume":"14","author":"E. Bestion","year":"2022","unstructured":"Bestion, E., Halfon, P., Mezouar, S., et al., Viruses, 2022, vol. 14, p. 1507.","journal-title":"Viruses"},{"key":"7665_CR5","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1038\/mt.2010.233","volume":"19","author":"A.V. Ulasov","year":"2011","unstructured":"Ulasov, A.V., Khramtsov, Y.V., Trusov, G.A., et al., Mol. Ther., 2011, vol. 19, pp. 103\u2013112.","journal-title":"Mol. Ther"},{"key":"7665_CR6","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1007\/s10529-018-2551-2","volume":"40","author":"S. Shahbazi","year":"2018","unstructured":"Shahbazi, S., Haghighipour, N., Soleymani, S., et al., Biotechnol. Lett., 2018, vol. 40, pp. 923\u2013931.","journal-title":"Biotechnol. Lett"},{"key":"7665_CR7","doi-asserted-by":"publisher","first-page":"1476","DOI":"10.1016\/j.ymthe.2017.01.024","volume":"25","author":"A. Hall","year":"2017","unstructured":"Hall, A., Lachelt, U., Bartek, J., et al., Mol. Ther., 2017, vol. 25, pp. 1476\u20131490.","journal-title":"Mol. Ther"},{"key":"7665_CR8","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1134\/S1607672911020062","volume":"437","author":"G.A. Trusov","year":"2011","unstructured":"Trusov, G.A., Ulasov, A.V., Beletskaia, E.A., et al., Dokl. Biochem. Biophys., 2011, vol. 437, pp. 77\u201379.","journal-title":"Dokl. Biochem. Biophys"},{"key":"7665_CR9","first-page":"1993","volume":"5","author":"T.D. Perrine","year":"1967","unstructured":"Perrine, T.D. and Landis, W.R., J. Polym. Sci., \n               Part A, 1967, vol. 5, pp. 1993\u20132003.","journal-title":"Part A"},{"key":"7665_CR10","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1023\/A:1016152831963","volume":"19","author":"K. Kunath","year":"2002","unstructured":"Kunath, K., von Harpe, A., Petersen, H., et al., Pharm. Res., 2002, vol. 19, pp. 810\u2013817.","journal-title":"Pharm. Res"},{"key":"7665_CR11","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1016\/j.talanta.2006.04.010","volume":"71","author":"X.W. Gong","year":"2007","unstructured":"Gong, X.W., Wei, D.Z., He, M.L., et al., Talanta, 2007, vol. 71, pp. 381\u2013384.","journal-title":"Talanta"}],"container-title":["Doklady Biochemistry and Biophysics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1134\/S1607672924601136.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1134\/S1607672924601136","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1134\/S1607672924601136.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T15:20:24Z","timestamp":1773760824000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1134\/S1607672924601136"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["7665"],"URL":"https:\/\/doi.org\/10.1134\/s1607672924601136","relation":{},"ISSN":["1607-6729","1608-3091"],"issn-type":[{"value":"1607-6729","type":"print"},{"value":"1608-3091","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4]]},"assertion":[{"value":"15 September 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 October 2024","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 October 2024","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 April 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"This work does not contain any studies involving human and animal subjects.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"ETHICS APPROVAL AND CONSENT TO PARTICIPATE"}},{"value":"The authors of this work declare that they have no conflicts of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"CONFLICT OF INTEREST"}}]}}