{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T03:56:18Z","timestamp":1774497378619,"version":"3.50.1"},"reference-count":18,"publisher":"Pleiades Publishing Ltd","issue":"1","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Dokl Biochem Biophys"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1134\/s1607672925600356","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T07:24:25Z","timestamp":1748935465000},"page":"302-308","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antitumor Effect of Helium Ions with Energy of 320 MeV\/Ion during Irradiation of Ehrlich Ascites Carcinoma Cells Ex Vivo"],"prefix":"10.1134","volume":"522","author":[{"given":"V. E.","family":"Balakin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. S.","family":"Strelnikova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O. M.","family":"Rozanova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. N.","family":"Smirnova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. A.","family":"Belyakova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. E.","family":"Shemyakov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"137","published-online":{"date-parts":[[2025,6,2]]},"reference":[{"key":"7699_CR1","doi-asserted-by":"publisher","first-page":"428","DOI":"10.14338\/ijpt-15-00027.1","volume":"2","author":"F. Tommasino","year":"2016","unstructured":"Tommasino, F., Scifoni, E., and Durante, M., New ions for therapy, Int. J. Particle Ther., 2016, vol. 2, no.\u00a03, pp. 428\u2013438. https:\/\/doi.org\/10.14338\/ijpt-15-00027.1","journal-title":"Int. J. Particle Ther."},{"key":"7699_CR2","doi-asserted-by":"publisher","first-page":"6784","DOI":"10.1088\/1361-6560\/aa7b12","volume":"62","author":"T. Tessonnier","year":"2017","unstructured":"Tessonnier, T., Mairani, A., Brons, S., Sala, P., Cerutti, F., Ferrari, A., Haberer, T., Debus, J., and Parodi, K., Helium ions at the heidelberg ion beam therapy center: comparisons between FLUKA Monte Carlo code predictions and dosimetric measurements, Phys. Med. Biol., 2017, vol. 62, no. 16, pp. 6784\u20136803. https:\/\/doi.org\/10.1088\/1361-6560\/aa7b12","journal-title":"Phys. Med. Biol."},{"key":"7699_CR3","doi-asserted-by":"publisher","unstructured":"Mairani, A., Mein, S., Blakely, E., Debus, J., Durante, M., Ferrari, A., Fuchs, H., Georg, D., Gyosshans, D.R., Guan, F., Haberer, T., Harrabi, S., Horst, F., Inaniwa, T., Karger, Ch.P., Mohan, R., Paganetti, H., Parodi, K., Sala, P., Schuy, Ch., Tessonnier, T., Titt, U., and Weber, U., Roadmap: helium ion therapy, Phys. Med. Biol., 2022, vol. 67, no. 15. https:\/\/doi.org\/10.1088\/1361-6560\/ac65d3","DOI":"10.1088\/1361-6560\/ac65d3"},{"key":"7699_CR4","doi-asserted-by":"publisher","first-page":"1995","DOI":"10.1118\/1.4944593","volume":"43","author":"M. Kr\u00e4mer","year":"2016","unstructured":"Kr\u00e4mer, M., Scifoni, E., Schuy, Ch., Rovituso, M., Tinganelli, W., Maier, A., Kaderka, R., Kraft-weyrather, W., Brons, S., Tessonnier, T., Parodi, K., and Durante, M., Helium ions for radiotherapy? Physical and biological verifications of a novel treatment modality, Med. Phys., 2016, vol. 43, no. 4, pp. 1995\u20132004. https:\/\/doi.org\/10.1118\/1.4944593","journal-title":"Med. Phys."},{"key":"7699_CR5","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1186\/s13014-017-0944-3","volume":"13","author":"T. Tessonnier","year":"2018","unstructured":"Tessonnier, T., Mairani, A., Chen, W., Sala, P., Cerutti, F., Ferrari, A., Haberer, T., Debus, J., and Parodi, K., Proton and helium ion radiotherapy for meningioma tumors: a Monte Carlo-based treatment planning comparison, Radiat. Oncol., 2018, vol. 13, no. 1, p. 2. https:\/\/doi.org\/10.1186\/s13014-017-0944-3","journal-title":"Radiat. Oncol."},{"key":"7699_CR6","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1159\/000099553","volume":"57","author":"R.P. Levy","year":"1991","unstructured":"Levy, R.P., Fabrikant, J.I., Frankel, K.A., Phillips, M.H., Lyman, J.T., Lawrence, J.H., and Tobias, C.A., Heavy-charged-particle radiosurgery of the pituitary gland: clinical results of 840 patients, Stereotactic Funct. Neurosurg., 1991, vol. 57, nos. 1\u20132, pp. 22\u201335. https:\/\/doi.org\/10.1159\/000099553","journal-title":"Stereotactic Funct. Neurosurg."},{"key":"7699_CR7","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1016\/j.ijrobp.2015.01.029","volume":"92","author":"K.K. Mishra","year":"2015","unstructured":"Mishra, K.K., Quivey, J.M., Daftari, I.K., Weinberg, V., Cole, T.B., Patel, K., Castro, J.R., Phillips, T.L., and Char, D.H., Long-term results of the UCSF-LBNL randomized trial: charged particle with helium ion versus iodine-125 plaque therapy for choroidal and ciliary body melanoma, Int. J. Radiat. Oncol., Biol., Phys., 2015, vol. 92, no. 2, pp. 376\u2013383. https:\/\/doi.org\/10.1016\/j.ijrobp.2015.01.029","journal-title":"Int. J. Radiat. Oncol., Biol., Phys."},{"key":"7699_CR8","doi-asserted-by":"publisher","first-page":"777","DOI":"10.1038\/s42254-021-00368-5","volume":"3","author":"M. Durante","year":"2021","unstructured":"Durante, M., Debus, J., and Loeffler, J.S., Physics and biomedical challenges of cancer therapy with accelerated heavy ions, Nat. Rev. Phys., 2021, vol. 3, no. 12, pp.\u00a0777\u2013790. https:\/\/doi.org\/10.1038\/s42254-021-00368-5","journal-title":"Nat. Rev. Phys."},{"key":"7699_CR9","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1134\/S0006350924700179","volume":"69","author":"O.M. Rozanova","year":"2024","unstructured":"Rozanova, O.M., Smirnova, E.N., Belyakova, T.A., Strelnikova, N.S., and Smirnov, A.V., Regularities of induction and growth of tumors in mice upon irradiation of ehrlich carcinoma cells ex vivo and in vivo with a pencil scanning beam of protons, Biophysics (Moscow), 2024, vol. 69, no. 1, pp. 156\u2013164. https:\/\/doi.org\/10.1134\/S0006350924700179","journal-title":"Biophysics (Moscow)"},{"key":"7699_CR10","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1134\/S1547477124702601","volume":"22","author":"T.A. Belyakova","year":"2025","unstructured":"Belyakova, T.A., Rozanova, O.M., and Smirnova, E.N., Modifying effect of 1-\u03b2-D-arabinofuranosylcytosine on the growth of the solid tumor Ehrlich carcinoma in mice under in vivo and ex vivo proton irradiation of cells, Phys. Part. Nucl. Lett., 2025, vol. 22, no. 2, pp.\u00a0477\u2013486. https:\/\/doi.org\/10.1134\/S1547477124702601","journal-title":"Phys. Part. Nucl. Lett."},{"key":"7699_CR11","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1258\/002367707780378212","volume":"41","author":"J.A. Smith","year":"2007","unstructured":"Smith, J.A., van den Broek, F.A.R., Martorell, J.C., Hackbarth, H., Ruksenas, O., and Zeller, W., Principles and practice in ethical review of animal experiments across Europe: summary of the report of a FELASA working group on ethical evaluation of animal experiments, Lab. Anim., 2007, vol. 41, no. 2, pp.\u00a0143\u2013160. https:\/\/doi.org\/10.1258\/002367707780378212","journal-title":"Lab. Anim."},{"key":"7699_CR12","first-page":"220","volume":"4","author":"N.I. Ryzhova","year":"2019","unstructured":"Ryzhova, N.I., Deryagina, V.P., and Savluchinskaya,\u00a0L.A., The value of the model of ehrlich adenocarcinoma in the study of the mechanisms of carcinogenesis, and antitumor activity of chemical and physical factors, Int. J. Appl. Fundam. Res., 2019, vol. 4, pp.\u00a0220\u2013227.","journal-title":"Int. J. Appl. Fundam. Res."},{"key":"7699_CR13","doi-asserted-by":"publisher","first-page":"s30","DOI":"10.1134\/s1607672924700765","volume":"513","author":"V.E. Balakin","year":"2023","unstructured":"Balakin, V.E., Belyakova, T.A., Rozanova, O.M., Smirnova, E.N., Strelnikova, N.S., and Kuznetsova, E.A., Anti-tumor effect of high doses of carbon ions and X-rays during irradiation of Ehrlich ascites carcinoma cells ex vivo, Dokl. Biochem. Biophys., 2023, vol. 513, no. S1, pp. s30\u2013s35. https:\/\/doi.org\/10.1134\/s1607672924700765","journal-title":"Dokl. Biochem. Biophys."},{"key":"7699_CR14","first-page":"1199","volume":"22","author":"B.S. Fedorenko","year":"1991","unstructured":"Fedorenko, B.S., Experimental studies of biological effectiveness of accelerated charged particles of relativistic energies, Phys. Part. Nucl., 1991, vol. 22, no. 5, pp.\u00a01199\u20131229.","journal-title":"Phys. Part. Nucl."},{"key":"7699_CR15","unstructured":"Rukovodstvo po provedeniyu doklinicheskikh issledovanii lekarstvennykh sredstv (Guidelines for Conducting Preclinical Studies of Medicines), Mironov, A.N., Bunatyan, A.S., and Vasil\u2019ev, A.N., Eds., Moscow: Gyif i K, 2012."},{"key":"7699_CR16","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1080\/09553009214551031","volume":"61","author":"I. Spadinger","year":"1992","unstructured":"Spadinger, I. and Palcic, B., The relative biological effectiveness of 60Co gamma-rays, 55 kVp X-rays, 250\u00a0kVp X-rays, and 11 MeV electrons at low doses, Int. J. Radiat. Biol., 1992, vol. 61, no. 3, pp. 345\u2013353. https:\/\/doi.org\/10.1080\/09553009214551031","journal-title":"Int. J. Radiat. Biol."},{"key":"7699_CR17","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1134\/s1607672923700229","volume":"511","author":"V.E. Balakin","year":"2023","unstructured":"Balakin, V.E., Rozanova, O.M., Smirnova, E.N., Belyakova, T.A., Strelnikova, N.S., Smirnov, A.V., and Shemyakov, A.E., Gyowth induction of solid Ehrlich ascitic carcinoma in mice after proton irradiation of tumor cells ex vivo, Dokl. Biochem. Biophys., 2023, vol.\u00a0511, no. 1, pp. 151\u2013155. https:\/\/doi.org\/10.1134\/s1607672923700229","journal-title":"Dokl. Biochem. Biophys."},{"key":"7699_CR18","first-page":"616","volume":"47","author":"A.N. Stukov","year":"2001","unstructured":"Stukov, A.N., Ivanova, M.A., and Nikitin, A.K., Tumor growth index as an integral criterion for the efficacy of antineoplastic therapy under experimental conditions, Vopr. Onkol., 2001, vol. 47, no. 5, pp. 616\u2013618.","journal-title":"Vopr. Onkol."}],"container-title":["Doklady Biochemistry and Biophysics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1134\/S1607672925600356.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1134\/S1607672925600356","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1134\/S1607672925600356.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T15:19:47Z","timestamp":1773760787000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1134\/S1607672925600356"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["7699"],"URL":"https:\/\/doi.org\/10.1134\/s1607672925600356","relation":{},"ISSN":["1607-6729","1608-3091"],"issn-type":[{"value":"1607-6729","type":"print"},{"value":"1608-3091","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"25 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 April 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 April 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 June 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Experiments with animals were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals (\n                      \n                      ). Protocols of experiments with animals were approved by the Commission on Biological Safety and Bioethics of the Theoretical and Experimental Biophysics of the Russian Academy of Sciences (protocol no. 11\/2023 dated February 8, 2023).","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"ETHICS APPROVAL AND CONSENT TO PARTICIPATE"}},{"value":"The authors of this work declare that they have no conflicts of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"CONFLICT OF INTEREST"}}]}}