{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T13:16:06Z","timestamp":1778591766648,"version":"3.51.4"},"reference-count":20,"publisher":"Pleiades Publishing Ltd","issue":"1","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Dokl Biochem Biophys"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1134\/s1607672925601684","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T19:19:17Z","timestamp":1774898357000},"page":"35-39","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Blockers of Acetylcholine Receptors Affect the Hypotensive Effect of the Central Loop Fragment of the WTX Toxin"],"prefix":"10.1134","volume":"526","author":[{"given":"M. S.","family":"Severyukhina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Ismailova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. R.","family":"Shaykhutdinova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. S.","family":"Egorova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. A.","family":"Dyachenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. I.","family":"Tsetlin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu. N.","family":"Utkin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"137","published-online":{"date-parts":[[2026,3,30]]},"reference":[{"key":"7800_CR1","doi-asserted-by":"publisher","first-page":"2778","DOI":"10.3390\/molecules24152778","volume":"24","author":"O. P\u00e9terfi","year":"2019","unstructured":"P\u00e9terfi, O., Boda, F., Szab\u00f3, Z., et al., Hypotensive snake venom components-a mini-review, Molecules, 2019, vol. 24, no. 15, p. 2778.","journal-title":"Molecules"},{"key":"7800_CR2","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.toxicon.2004.09.014","volume":"45","author":"A.Y. Ogay","year":"2005","unstructured":"Ogay, A.Y., Rzhevsky, D.I., Murashev, A.N., et al., Weak neurotoxin from Naja kaouthia cobra venom affects haemodynamic regulation by acting on acetylcholine receptors, Toxicon, 2005, vol. 45, no. 1, pp. 93\u201399.","journal-title":"Toxicon"},{"key":"7800_CR3","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1111\/jnc.13975","volume":"142","author":"P. Kessler","year":"2017","unstructured":"Kessler, P., Marchot, P., Silva, M., et al., The three finger toxin fold: a multifunctional structural scaffold able to modulate cholinergic functions, J. Neurochem., 2017, vol. 142, no. s2, pp. 7\u201318.","journal-title":"J. Neurochem."},{"key":"7800_CR4","doi-asserted-by":"crossref","unstructured":"Nirthanan, S., Snake three-finger \u03b1-neurotoxins and nicotinic acetylcholine receptors: molecules, mechanisms and medicine, Biochem. Pharmacol., 2020, vol.\u00a0181, p. 114168.","DOI":"10.1016\/j.bcp.2020.114168"},{"key":"7800_CR5","doi-asserted-by":"publisher","first-page":"15810","DOI":"10.1074\/jbc.M100788200","volume":"276","author":"Y.N. Utkin","year":"2001","unstructured":"Utkin, Y.N., Kukhtina, V.V., Kryukova, E.V., et al., Weak toxin\u201d from Naja kaouthia is a nontoxic antagonist of alpha 7 and muscle-type nicotinic acetylcholine receptors, J. Biol. Chem., 2001, vol. 276, no. 19, pp.\u00a015810\u201315815.","journal-title":"J. Biol. Chem."},{"key":"7800_CR6","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1016\/S0041-0101(02)00388-4","volume":"41","author":"S. Nirthanan","year":"2003","unstructured":"Nirthanan, S., Gopalakrishnakone, P., and Gwee, M.C., Non-conventional toxins from Elapid venoms, Toxicon, 2003, vol. 41, no. 4, pp. 397\u2013407.","journal-title":"Toxicon"},{"key":"7800_CR7","doi-asserted-by":"publisher","first-page":"5065","DOI":"10.1111\/j.1742-4658.2009.07203.x","volume":"276","author":"D.Y. Mordvintsev","year":"2009","unstructured":"Mordvintsev, D.Y., Polyak, Y.L., Rodionov, D.I., et\u00a0al., Weak toxin WTX from Naja kaouthia cobra venom interacts with both nicotinic and muscarinic acetylcholine receptors, FEBS J., 2009, vol. 276, no. 18, pp.\u00a05065\u20135075.","journal-title":"FEBS J."},{"key":"7800_CR8","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1111\/jnc.15821","volume":"168","author":"K. Targosova","year":"2024","unstructured":"Targosova, K., Kucera, M., Fazekas, T., Kilianova, Z., Stankovicova, T., and Hrabovska, A., \u03b17 nicotinic receptors play a role in regulation of cardiac hemodynamics, J. Neurochem., 2024, vol. 168, no. 4, pp. 414\u2013427. https:\/\/doi.org\/10.1111\/jnc.15821","journal-title":"J. Neurochem."},{"key":"7800_CR9","doi-asserted-by":"publisher","first-page":"3745","DOI":"10.1038\/s41598-025-87212-3","volume":"15","author":"H.C. Saternos","year":"2025","unstructured":"Saternos, H.C., Forero, K.V., Meqdad, M.A., et al., Muscarinic acetylcholine receptor 3 localized to primary endothelial cilia regulates blood pressure and cognition, Sci. Rep., 2025, vol. 15, no. 1, p. 3745. https:\/\/doi.org\/10.1038\/s41598-025-87212-3","journal-title":"Sci. Rep."},{"key":"7800_CR10","doi-asserted-by":"publisher","unstructured":"Severyukhina, M.S., Ojomoko, L.O., Shelukhina, I.V., et al., Non-conventional toxin WTX and its disulfide-fixed synthetic fragments: Interaction with nicotinic acetylcholine receptors and reduction of blood pressure, Int. J. Biol. Macromol., 2025, vol. 288, p. 138626. https:\/\/doi.org\/10.1016\/j.ijbiomac.2024.138626","DOI":"10.1016\/j.ijbiomac.2024.138626"},{"key":"7800_CR11","doi-asserted-by":"publisher","first-page":"23616","DOI":"10.1074\/jbc.m115.656595","volume":"290","author":"E.N. Lyukmanova","year":"2015","unstructured":"Lyukmanova, E.N., Shenkarev, Z.O., Shulepko, M.A., et al., Structural insight into specificity of interactions between nonconventional three-finger weak toxin from Naja kaouthia (WTX) and muscarinic acetylcholine receptors, J. Biol. Chem., 2015, vol. 290, no. 39, pp.\u00a023616\u201323630. https:\/\/doi.org\/10.1074\/jbc.m115.656595","journal-title":"J. Biol. Chem."},{"key":"7800_CR12","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.ejphar.2005.06.050","volume":"519","author":"S. Motoshima","year":"2005","unstructured":"Motoshima, S., Suemaru, K., Kawasaki, Yo., et al., Effects of \u03b14\u03b22 and \u03b17 nicotinic acetylcholine receptor antagonists on place aversion induced by naloxone in single-dose morphine-treated rats, Eur. J. Pharmacol., 2005, vol. 519, nos. 1\u20132, pp. 91\u201395. https:\/\/doi.org\/10.1016\/j.ejphar.2005.06.050","journal-title":"Eur. J. Pharmacol."},{"key":"7800_CR13","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.biochi.2023.10.011","volume":"216","author":"E.R. Shaykhutdinova","year":"2024","unstructured":"Shaykhutdinova, E.R., Severyukhina, M.S., Kholoshenko, I.V., et al., Anti-smoking drugs cytisine and varenicline reduce cardiac reperfusion injury in rat model of myocardial ischemia, Biochimie, 2024, vol. 216, pp.\u00a0108\u2013119. https:\/\/doi.org\/10.1016\/j.biochi.2023.10.011","journal-title":"Biochimie"},{"key":"7800_CR14","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1016\/j.drugalcdep.2012.12.021","volume":"131","author":"E.M. Jutkiewicz","year":"2013","unstructured":"Jutkiewicz, E.M., Rice, K.C., Carroll, F.I., et al., Patterns of nicotinic receptor antagonism II: Cardiovascular effects in rats, Drug Alcohol Dependence, 2013, vol. 131, no. 3, pp. 284\u2013297. https:\/\/doi.org\/10.1016\/j.drugalcdep.2012.12.021","journal-title":"Drug Alcohol Dependence"},{"key":"7800_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1134\/S1068162023010211","volume":"49","author":"A.V. Osipov","year":"2023","unstructured":"Osipov, A.V., Averin, A.S., Shaykhutdinova, E.R., et al., Muscarinic and nicotinic acetylcholine receptors in the regulation of the cardiovascular system, Russ. J. Bioorg. Chem., 2023, vol. 49, no. 1, pp. 1\u201318.","journal-title":"Russ. J. Bioorg. Chem."},{"key":"7800_CR16","doi-asserted-by":"publisher","first-page":"s389","DOI":"10.33549\/physiolres.935270","volume":"73","author":"E. Dolej\u0161\u00ed","year":"2024","unstructured":"Dolej\u0161\u00ed, E., Janou\u0161kov\u00e1, A., and Jakub\u00edk, J., Muscarinic receptors in cardioprotection and vascular tone regulation, Physiol. Res., 2024, vol. 73, no. S1, pp. s389\u2013s400. https:\/\/doi.org\/10.33549\/physiolres.935270","journal-title":"Physiol. Res."},{"key":"7800_CR17","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1111\/j.1476-5381.1997.tb06837.x","volume":"120","author":"A.M. Gurney","year":"1997","unstructured":"Gurney, A.M. and Rang, H.P., The channel-blocking action of methonium compounds on rat submandibular ganglion cells, Br. J. Pharmacol., 1997, vol. 120, no. s4, pp. 471\u2013490. https:\/\/doi.org\/10.1111\/j.1476-5381.1997.tb06837.x","journal-title":"Br. J. Pharmacol."},{"key":"7800_CR18","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.vascn.2012.05.003","volume":"66","author":"R. Nirogi","year":"2012","unstructured":"Nirogi, R., Kandikere, V., Bhyrapuneni, G., et al., Methyllycaconitine: a non-radiolabeled ligand for mapping \u03b17 neuronal nicotinic acetylcholine receptors\u2014in vivo target localization and biodistribution in rat brain, J. Pharmacol. Toxicol. Methods, 2012, vol. 66, no. 1, pp. 22\u201328. https:\/\/doi.org\/10.1016\/j.vascn.2012.05.003","journal-title":"J. Pharmacol. Toxicol. Methods"},{"key":"7800_CR19","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1007\/978-3-642-23274-9_13","volume":"208","author":"R.D. Harvey","year":"2012","unstructured":"Harvey, R.D., Muscarinic receptor agonists and antagonists: effects on cardiovascular function, Handb. Exp. Pharmacol., 2012, vol. 208, pp. 299\u2013316. https:\/\/doi.org\/10.1007\/978-3-642-23274-9_13","journal-title":"Handb. Exp. Pharmacol."},{"key":"7800_CR20","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.neulet.2009.06.081","volume":"462","author":"Y.-F. Li","year":"2009","unstructured":"Li, Y.-F., Lacroix, C., and Freeling, J., Specific subtypes of nicotinic cholinergic receptors involved in sympathetic and parasympathetic cardiovascular responses, Neurosci. Lett., 2009, vol. 462, no. 1, pp. 20\u201323. https:\/\/doi.org\/10.1016\/j.neulet.2009.06.081","journal-title":"Neurosci. Lett."}],"container-title":["Doklady Biochemistry and Biophysics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1134\/S1607672925601684.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1134\/S1607672925601684","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1134\/S1607672925601684.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T12:49:28Z","timestamp":1778590168000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1134\/S1607672925601684"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["7800"],"URL":"https:\/\/doi.org\/10.1134\/s1607672925601684","relation":{},"ISSN":["1607-6729","1608-3091"],"issn-type":[{"value":"1607-6729","type":"print"},{"value":"1608-3091","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"20 October 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 October 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 November 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 March 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All animal experiments were approved by the Commission of the Shemyakin\u2013Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences for the Control over the Care and Use of Laboratory Animals (protocol no. 1044\/25 dated August 26, 2025).","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"ETHICS APPROVAL AND CONSENT TO PARTICIPATE"}},{"value":"The authors of this work declare that they have no conflicts of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"CONFLICT OF INTEREST"}}]}}