{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:49:51Z","timestamp":1791298191986,"version":"4.1.0"},"reference-count":38,"publisher":"BMJ","issue":"2","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Arch Dis Child Fetal Neonatal Ed"],"accepted":{"date-parts":[[2017,5,22]]},"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>Preterm infants have relative adrenal and kidney immaturity. Recently, we linked their urine sodium loss to a hypoaldosteronism at variance with an appropriate stimulation of the renin-angiotensin system. To investigate this defective aldosterone secretion, we analyse the biosynthesis pathways of adrenal steroids in neonates according to gestational age (GA).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Design<\/jats:title>\n                  <jats:p>Multicentre study (Premaldo) including 152 neonates classified into three groups: group 1 (very preterm (VPT)): &lt;33\u2009 gestational weeks (GW); group 2 (preterm (PT)): 33\u201336 GW and group 3 (term (T)): \u2265GW.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Method<\/jats:title>\n                  <jats:p>Steroidomic profiles of mineralocorticoids, glucocorticoids and adrenal androgens were established from umbilical cord at birth (n=152) and peripheral blood at day 3 (n=70) using a recently developed liquid chromatography mass spectrometry method (LC-MS\/MS). The enzymatic activity of each biosynthesis step was estimated by the product-to-substrate ratio.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>At birth, VPT infants exhibit a global defect in adrenal steroid synthesis pathways leading to lower levels of aldosterone, cortisol and androstenedione than in term infants. This defect was strongly related to GA. On day 3, steroid precursors (progesterone, 11-deoxycorticosterone (DOC), 17-hydroxyprogesterone(17-OH-P) and 11-deoxycortisol (S)) were higher in VPT and negatively correlated with GA. Despite of precursors\u2019 accumulation, aldosterone and cortisol were similar in the three groups. At birth and day 3, a low cortisol\/11-deoxycortisol ratio was found in preterm infants, suggesting an 11-beta-hydroxylase activity (<jats:italic>CYP11B1<\/jats:italic>) deficiency.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>At birth, VPT infants exhibit a global deficit in mineralocorticoids, glucocorticoids and adrenal androgens that attenuates on day 3 of life. Steroid profiling using LC-MS\/MS provides evidence for a partial defect in 11-hydroxylase along with prematurity.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/archdischild-2016-312457","type":"journal-article","created":{"date-parts":[[2017,7,26]],"date-time":"2017-07-26T20:50:20Z","timestamp":1501102220000},"page":"F143-F151","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":33,"title":["Alterations of adrenal steroidomic profiles in preterm infants at birth"],"prefix":"10.1136","volume":"103","author":[{"given":"Simon","family":"Travers","sequence":"first","affiliation":[{"name":"Inserm, U1185, Le Kremlin-Bic\u00eatre, France"},{"name":"Fac Med Paris-Sud, Univ, Paris-Sud, Universit\u00e9 Paris Saclay, Le Kremlin-Bic\u00eatre, France"},{"name":"Service de G\u00e9n\u00e9tique Mol\u00e9culaire, Pharmacog\u00e9n\u00e9tique et Hormonologie, H\u00f4pital de Bic\u00eatre, H\u00f4pitaux Universitaires Paris Sud, Assistance Publique\u2019H\u00f4pitaux de Paris, Le Kremlin Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laetitia","family":"Martinerie","sequence":"additional","affiliation":[{"name":"Inserm, U1185, Le Kremlin-Bic\u00eatre, France"},{"name":"Service d\u2019Endocrinologie P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Assistance Publique H\u00f4pitaux de Paris, Paris, France"},{"name":"PremUp Foundation, Paris, France"},{"name":"Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Boileau","sequence":"additional","affiliation":[{"name":"PremUp Foundation, Paris, France"},{"name":"Service de R\u00e9animation N\u00e9onatale, CH Poissy St\u2019Germain en\u2019Laye, Poissy, France"},{"name":"EA 7285, UFR des Sciences de la Sant\u00e9, Simone Veil, Universit\u00e9 Versailles St-Quentin en Yvelines, Montigny le Bretonneux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Lomb\u00e8s","sequence":"additional","affiliation":[{"name":"Inserm, U1185, Le Kremlin-Bic\u00eatre, France"},{"name":"Fac Med Paris-Sud, Univ, Paris-Sud, Universit\u00e9 Paris Saclay, Le Kremlin-Bic\u00eatre, France"},{"name":"PremUp Foundation, Paris, France"},{"name":"Service d\u2019Endocrinologie et Maladies de la Reproduction, H\u00f4pital de Bic\u00eatre, H\u00f4pitaux Universitaires Paris Sud, Assistance Publique\u2019H\u00eapitaux de Paris, Le Kremlin Bic\u00eatre, France"},{"name":"Institut Biom\u00e9dical de Bic\u00eatre, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Pussard","sequence":"additional","affiliation":[{"name":"Inserm, U1185, Le Kremlin-Bic\u00eatre, France"},{"name":"Fac Med Paris-Sud, Univ, Paris-Sud, Universit\u00e9 Paris Saclay, Le Kremlin-Bic\u00eatre, France"},{"name":"Service de G\u00e9n\u00e9tique Mol\u00e9culaire, Pharmacog\u00e9n\u00e9tique et Hormonologie, H\u00f4pital de Bic\u00eatre, H\u00f4pitaux Universitaires Paris Sud, Assistance Publique\u2019H\u00f4pitaux de Paris, Le Kremlin Bic\u00eatre, France"},{"name":"Institut Biom\u00e9dical de Bic\u00eatre, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2017,7,26]]},"reference":[{"key":"2025090514344396000_103.2.F143.1","doi-asserted-by":"publisher","DOI":"10.1016\/S1084-2756(03)00042-3"},{"key":"2025090514344396000_103.2.F143.2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(79)80737-4"},{"key":"2025090514344396000_103.2.F143.3","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.jsbmb.2015.05.008","article-title":"Aldosterone biosynthesis in the human adrenal cortex and associated disorders","volume":"153","author":"Nakamura","year":"2015","journal-title":"J Steroid Biochem Mol Biol"},{"key":"2025090514344396000_103.2.F143.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2013.11.022"},{"key":"2025090514344396000_103.2.F143.5","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.mce.2014.02.010","article-title":"Development of adrenal cortical zonation and expression of key elements of adrenal androgen production in the chimpanzee (Pan troglodytes) from birth to adulthood","volume":"387","author":"Parker","year":"2014","journal-title":"Mol Cell Endocrinol"},{"key":"2025090514344396000_103.2.F143.6","doi-asserted-by":"publisher","DOI":"10.1210\/en.139.12.5144"},{"key":"2025090514344396000_103.2.F143.7","doi-asserted-by":"publisher","DOI":"10.1210\/jc.87.3.1194"},{"key":"2025090514344396000_103.2.F143.8","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1203\/00006450-199608000-00021","article-title":"Serum cortisol, dehydroepiandrosterone sulfate, and steroid-binding globulins in preterm neonates: effect of gestational age and dexamethasone therapy","volume":"40","author":"Kari","year":"1996","journal-title":"Pediatr Res"},{"key":"2025090514344396000_103.2.F143.9","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-69-6-1133"},{"key":"2025090514344396000_103.2.F143.10","doi-asserted-by":"publisher","DOI":"10.1210\/jc.78.2.266"},{"key":"2025090514344396000_103.2.F143.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2014.04.011"},{"key":"2025090514344396000_103.2.F143.12","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(96)70404-3"},{"key":"2025090514344396000_103.2.F143.13","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200108000-00005"},{"key":"2025090514344396000_103.2.F143.14","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e3181b1bbec"},{"key":"2025090514344396000_103.2.F143.15","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-1498"},{"key":"2025090514344396000_103.2.F143.16","first-page":"CD000503","article-title":"Restricted versus liberal water intake for preventing morbidity and mortality in preterm infants","volume":"12","author":"Bell","year":"2014","journal-title":"Cochrane Database Syst Rev"},{"key":"2025090514344396000_103.2.F143.17","doi-asserted-by":"crossref","first-page":"4074","DOI":"10.1210\/jc.2015-2272","article-title":"Aldosterone-Signaling defect exacerbates sodium wasting in very preterm neonates: the Premaldo Study","volume":"100","author":"Martinerie","year":"2015","journal-title":"J Clin Endocrinol Metab"},{"key":"2025090514344396000_103.2.F143.18","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.jsbmb.2016.06.005","article-title":"Multiplexed steroid profiling of gluco- and mineralocorticoids pathways using a liquid chromatography tandem mass spectrometry method","volume":"165","author":"Travers","year":"2017","journal-title":"J Steroid Biochem Mol Biol"},{"key":"2025090514344396000_103.2.F143.19","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-013-0558-1"},{"key":"2025090514344396000_103.2.F143.20","doi-asserted-by":"publisher","DOI":"10.1038\/jp.2009.24"},{"key":"2025090514344396000_103.2.F143.21","doi-asserted-by":"publisher","DOI":"10.1159\/000314970"},{"key":"2025090514344396000_103.2.F143.22","doi-asserted-by":"crossref","first-page":"69","DOI":"10.3109\/07435809509030422","article-title":"Immunohistochemical evaluation of the cellular localization and ontogeny of 3 beta-hydroxysteroid dehydrogenase\/delta 5-4 isomerase in the human fetal adrenal gland","volume":"21","author":"Parker","year":"1995","journal-title":"Endocr Res"},{"key":"2025090514344396000_103.2.F143.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2005.08.004"},{"key":"2025090514344396000_103.2.F143.24","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1111\/apha.12187","article-title":"Foetal and placental 11\u03b2-HSD2: a hub for developmental programming","volume":"210","author":"Cottrell","year":"2014","journal-title":"Acta Physiol"},{"key":"2025090514344396000_103.2.F143.25","doi-asserted-by":"publisher","DOI":"10.1203\/01.pdr.0000203106.59832.7a"},{"key":"2025090514344396000_103.2.F143.26","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2008-0516"},{"key":"2025090514344396000_103.2.F143.27","doi-asserted-by":"publisher","DOI":"10.1177\/1933719108324140"},{"key":"2025090514344396000_103.2.F143.28","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2012.06.012"},{"key":"2025090514344396000_103.2.F143.29","doi-asserted-by":"publisher","DOI":"10.1515\/JPEM.2009.22.11.1027"},{"key":"2025090514344396000_103.2.F143.30","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e3181a1f0e6"},{"key":"2025090514344396000_103.2.F143.31","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-3782(97)00037-6"},{"key":"2025090514344396000_103.2.F143.32","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2009.11.007"},{"key":"2025090514344396000_103.2.F143.33","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.mce.2016.06.015","article-title":"Temporal and spatial distribution of mast cells and steroidogenic enzymes in the human fetal adrenal","volume":"434","author":"Naccache","year":"2016","journal-title":"Mol Cell Endocrinol"},{"key":"2025090514344396000_103.2.F143.34","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200108000-00005"},{"key":"2025090514344396000_103.2.F143.35","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1203\/00006450-198805000-00018","article-title":"Plasma mineralocorticoids, glucocorticoids, and progestins in premature infants: longitudinal study during the first week of life","volume":"23","author":"Doerr","year":"1988","journal-title":"Pediatr Res"},{"key":"2025090514344396000_103.2.F143.36","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-004-1572-0"},{"key":"2025090514344396000_103.2.F143.37","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1159\/000244213","article-title":"Hepatic biotransformation capacity in low-birth-weight infants as measured with the [15N]methacetin urine test: influences of gestational age, postnatal age, and intrauterine growth retardation","volume":"68","author":"Boehm","year":"1995","journal-title":"Biol Neonate"},{"key":"2025090514344396000_103.2.F143.38","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-0473"}],"container-title":["Archives of Disease in Childhood - Fetal and Neonatal Edition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/archdischild-2016-312457","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T21:34:53Z","timestamp":1757108093000},"score":1,"resource":{"primary":{"URL":"https:\/\/fn.bmj.com\/lookup\/doi\/10.1136\/archdischild-2016-312457"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,26]]},"references-count":38,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,2,19]]},"published-print":{"date-parts":[[2018,3]]}},"alternative-id":["10.1136\/archdischild-2016-312457"],"URL":"https:\/\/doi.org\/10.1136\/archdischild-2016-312457","relation":{},"ISSN":["1359-2998","1468-2052"],"issn-type":[{"value":"1359-2998","type":"print"},{"value":"1468-2052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,26]]}}}