{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,27]],"date-time":"2025-04-27T15:10:04Z","timestamp":1745766604835,"version":"3.40.4"},"reference-count":8,"publisher":"BMJ","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BMJ Case Reports"],"abstract":"<jats:p><jats:italic>Plasmodium knowlesi<\/jats:italic>malaria is an uncommon, but highly prevalent parasitic infection in parts of Malaysia. This is the case of a 14-year-old Singaporean boy presenting to our emergency department with an 11-day history of fever following a school trip to Malaysia. Hepatosplenomegaly was the only clinical finding; laboratory tests showed thrombocytopaenia, lymphopaenia, mild anaemia and liver transaminitis. Specific malaria antigen tests were negative, but the peripheral blood film showed plasmodia with atypical features, with a parasite load of 0.5%. PCR confirmed the diagnosis of<jats:italic>P knowlesi<\/jats:italic>. The patient was successfully treated with chloroquine. The clinical course of<jats:italic>P knowlesi<\/jats:italic>malaria is indistinguishable from that of<jats:italic>Plasmodium falciparum<\/jats:italic>. This case highlights the importance of taking detailed travel history, careful examination of malaria blood films and judicious use of molecular techniques. Antigen tests alone may have missed a malaria diagnosis altogether, while blood film examination may wrongly identify the species as<jats:italic>Plasmodium malariae<\/jats:italic>or<jats:italic>P falciparum<\/jats:italic>. Third-generation PCR assays can be used to reliably identify<jats:italic>P knowlesi<\/jats:italic>.<\/jats:p>","DOI":"10.1136\/bcr-2013-009558","type":"journal-article","created":{"date-parts":[[2013,4,22]],"date-time":"2013-04-22T14:30:28Z","timestamp":1366641028000},"page":"bcr2013009558","source":"Crossref","is-referenced-by-count":8,"title":["<i>Plasmodium knowlesi<\/i>infection: a diagnostic challenge"],"prefix":"10.1136","volume":"2013","author":[{"given":"Lijia","family":"Fan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shir Ying","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evelyn","family":"Koay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Harkensee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2013,4,22]]},"reference":[{"key":"2025042707384572000_2013.apr22_1.bcr2013009558.1","first-page":"166","article-title":"Plasmodium knowlesi malaria in Malaysia","volume":"2013","author":"Singh","year":"2010","journal-title":"Med J Malaysia"},{"key":"2025042707384572000_2013.apr22_1.bcr2013009558.2","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1086\/524888","article-title":"Plasmodium knowlesi malaria in humans is widely distributed and potentially life threatening","volume":"2013","author":"Cox-Singh","year":"2008","journal-title":"Clin Infect Dis"},{"key":"2025042707384572000_2013.apr22_1.bcr2013009558.3","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.pt.2008.06.001","article-title":"Knowlesi malaria: newly emergent and of public health importance?","volume":"2013","author":"Cox-Singh","year":"2008","journal-title":"Trends Parasitol"},{"key":"2025042707384572000_2013.apr22_1.bcr2013009558.4","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1086\/524889","article-title":"Plasmodium knowlesi: the fifth human malaria parasite","volume":"2013","author":"White","year":"2008","journal-title":"Clin Infect Dis"},{"key":"2025042707384572000_2013.apr22_1.bcr2013009558.5","unstructured":"Malaria Atlas Project. http:\/\/www.map.ox.ac.uk\/ (accessed 17 Feb 2012)."},{"key":"2025042707384572000_2013.apr22_1.bcr2013009558.6","doi-asserted-by":"crossref","first-page":"814","DOI":"10.3201\/eid1405.070863","article-title":"Naturally acquired human Plasmodium knowlesi infection, Singapore","volume":"2013","author":"Ng","year":"2008","journal-title":"Emerg Infect Dis"},{"key":"2025042707384572000_2013.apr22_1.bcr2013009558.7","doi-asserted-by":"crossref","first-page":"927","DOI":"10.4269\/ajtmh.2009.80.927","article-title":"Monkey malaria in humans: a diagnostic dilemma with conflicting laboratory data","volume":"2013","author":"Ong","year":"2009","journal-title":"Am J Trop Med Hyg"},{"key":"2025042707384572000_2013.apr22_1.bcr2013009558.8","doi-asserted-by":"crossref","first-page":"814","DOI":"10.3201\/eid1705.101489","article-title":"Plasmodium knowlesi malaria in children","volume":"2013","author":"Barber","year":"2011","journal-title":"Emerg Infect Dis"}],"container-title":["BMJ Case Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/bcr-2013-009558","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,27]],"date-time":"2025-04-27T14:38:53Z","timestamp":1745764733000},"score":1,"resource":{"primary":{"URL":"https:\/\/casereports.bmj.com\/lookup\/doi\/10.1136\/bcr-2013-009558"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,22]]},"references-count":8,"alternative-id":["10.1136\/bcr-2013-009558"],"URL":"https:\/\/doi.org\/10.1136\/bcr-2013-009558","relation":{},"ISSN":["1757-790X"],"issn-type":[{"type":"electronic","value":"1757-790X"}],"subject":[],"published":{"date-parts":[[2013,4,22]]}}}